Chondrocytes cultured with 0, 25 or 50 µg/ml of 7α,25-DHC for 48 h were resuspended in binding buffer, followed by the addition of annexin V-fluorescein isothiocyanate and propidium iodide (PI; Cell Signaling Technology). Apoptotic proportions were assessed by BD Cell Quest version 3.3 (Becton Dickinson, USA).
Annexin 5 fluorescein isothiocyanate
Manufactured by Cell Signaling Technology
Annexin V-fluorescein isothiocyanate is a recombinant protein that binds to phosphatidylserine, a phospholipid that becomes exposed on the surface of cells undergoing apoptosis. This product is used to detect and quantify apoptotic cells in various applications, such as flow cytometry and fluorescence microscopy.
Automatically generated - may contain errors
2 protocols using annexin 5 fluorescein isothiocyanate
1
Apoptotic Chondrocyte Assessment
2
Annexin V–FITC Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure the rate of cell apoptosis, we stained H9c2 cells with Annexin V–fluorescein isothiocyanate and propidium iodide (Cell Signaling Technology). The stained cells were washed and then investigated using a Cytomics FC500 flow cytometry analyzer (Beckman Coulter, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!