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Eaac1

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EAAC1 is a transporters protein that is responsible for the uptake of the neurotransmitter glutamate in neurons and glial cells. It plays a crucial role in regulating the levels of glutamate in the synaptic cleft, which is essential for proper neuronal function and signaling.

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Lab products found in correlation

Hrp conjugated anti mouse igg, by Cell Signaling Technology (3 mentions) Hrp conjugated anti rabbit igg, by Cell Signaling Technology (3 mentions) β actin, by Novus Biologicals (2 mentions) Recombinant β type nrg1, by ProSpec (2 mentions) Phospho tau ser422, by Cell Signaling Technology (2 mentions) Cocl2 c8661, by Merck Group (2 mentions) Myc tag, by Cell Signaling Technology (2 mentions) β type nrg1, by ProSpec (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Wortmannin, by Merck Group (1 mentions) Glt 1, by Cell Signaling Technology (1 mentions) Horseradish peroxidase linked anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions) Tgn 020, by Bio-Techne (1 mentions) Horseradish peroxidase linked anti mouse secondary antibody, by Cell Signaling Technology (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Cleaved caspase 3, by Santa Cruz Biotechnology (1 mentions)

4 protocols using eaac1

1

Recombinant NRG1 and Antibody Sources

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The NRG1 used in this study was a recombinant polypeptide containing the entire EGF domain of the β-type NRG1 from PROSPEC (East Brunswick, NJ, USA). Antibodies were supplied by Millipore Corporation (Chemicon, MA, USA) (EAAT3 (EAAC1), MAB1587), Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA) (β-actin, sc-47778; HRP-conjugated anti-rabbit IgG, sc-2004; and HRP-conjugated anti-mouse IgG, sc-2005), and Cell Signaling Technology (CST, MA, USA) (EAAC1, #12179). H2O2 (216763) and Rhodamine 123 (Rh123, 83702) were purchased from Sigma-Aldrich (St. Louis, MO, CA, USA).
Lee J.H., Yoo J.Y., Kim H.B., Yoo H.I., Song D.Y., Min S.S., Baik T.K, & Woo R.S. (2018). Neuregulin1 Attenuates H2O2-Induced Reductions in EAAC1 Protein Levels and Reduces H2O2-Induced Oxidative Stress. Neurotoxicity Research, 35(2), 401-409.
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2

Neuregulin-1 Signaling Pathway Analysis

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Recombinant β-type NRG1 was purchased from ProSpec (East Brunswick, NJ, USA). Antibodies were obtained from Millipore Corporation (Chemicon, MA, USA) (EAAT3 (EAAC1), MAB1587), Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA) (Bcl-XL, sc-8392; p53, sc-126; β-actin, sc-47778), Novus Biologicals (Centennial, CO, USA) (HIF-1α, NB100-131; Tau, NBP-25613), ThermoFisher scientific (Waltham, MA, USA) (Phospho-Tau (AT8), #MN1020), Mybiosoure (San Diego, CA, USA) (Phospho-Tau (ser422), #A11008), and Cell Signaling Technology (CST, MA, USA) (Caspase 3, #9662 s; cleaved caspase 3, # 9661 s; EAAC1, #12,179; Myc-tag, #2276; HRP-conjugated anti-rabbit IgG, #7076 s; HRP-conjugated anti-mouse IgG, #7074 s). CoCl2 (C8661) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Kim H.B., Yoo J.Y., Yoo S.Y., Lee J.H., Chang W., Kim H.S., Baik T.K, & Woo R.S. (2020). Neuregulin-1 inhibits CoCl2-induced upregulation of excitatory amino acid carrier 1 expression and oxidative stress in SH-SY5Y cells and the hippocampus of mice. Molecular Brain, 13, 153.
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3

Alzheimer's Disease Cell Experiments

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MTT (3-(4,5-dimethylthiazol-2-yl)2-,5-diphenyltetrazolium bromide), insulin, wortmannin were purchased from Sigma-Aldrich (St. Louis, MO). Antibody against AQP4 (sc-32739) was purchased from Santa Cruz Biotechnology (California, USA), cleaved caspase-3 (#9664), GLT-1 (#3838), GLAST (#5684), EAAC1 (#14501), GFAP (#80788), and horseradish peroxidase-linked anti-mouse secondary antibody (#7076), horseradish peroxidase-linked anti-rabbit secondary antibody (#7074) were purchased from Cell Signaling Technology (Beverly, MA, USA). The β-amyloid25-35 (Aβ25-35) and β-amyloid1-42 (Aβ1-42) were purchased from Abcam Biotechnology (Cambridge, UK). TGN-020 was purchased from Tocris Bioscience (Bristol, United Kingdom). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM) were purchased from the American Type Culture Collection (Manassas, VA, USA).
Park S.H., Lee J.Y., Jhee K.H, & Yang S.A. (2020). Amyloid-ß peptides inhibit the expression of AQP4 and glutamate transporter EAAC1 in insulin-treated C6 glioma cells. Toxicology Reports, 7, 1083-1089.
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4

Recombinant NRG1 Signaling Pathway

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Recombinant β-type NRG1 was purchased from ProSpec (East Brunswick, NJ, USA). Antibodies were obtained from Millipore Corporation (Chemicon, MA, USA) (EAAT3 (EAAC1), MAB1587), Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA) (Bcl-X L , sc-8392; p53, sc-126; β-actin, sc-47778), Novus Biologicals (Centennial, CO, USA) (HIF-1α, NB100-131; Tau, NBP-25613), ThermoFisher scienti c (Waltham, MA, USA) (Phospho-Tau (AT8), #MN1020), Mybiosoure (San Diego, CA, USA) (Phospho-Tau (ser422), #A11008), and Cell Signaling Technology (CST, MA, USA) (Caspase 3, #9662s; cleaved caspase 3, # 9661s; EAAC1, #12179; Myc-tag, #2276; HRP-conjugated anti-rabbit IgG, #7076s; HRP-conjugated anti-mouse IgG, #7074s). CoCl 2 (C8661) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Kim H., Yoo J., Yoo S., Lee J., Chang W.S., Kim H., Baik T., & Woo R. (2020). Neuregulin-1 inhibits CoCl2-induced upregulation of excitatory amino acid carrier 1 expression and oxidative stress in SH-SY5Y cells and the hippocampus of mice.
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