Anemoside B4 (B4), whose chemical structure is shown in Figure 1(a) (Batch No. 20161107, purity of 98% using HPLC determination, the chromatogram of anemoside B4 is shown in Figure 1(b) ) was presented by Professor Yu-Lin Feng from the Phytochemical Department of our university. Prednisolone (pred.) (11-beta,17-alpha; 11,17,21-trihydroxypregna-1,4-diene-3,20-dione; (11beta)-11,17-dihydroxy- 3,20- dioxopregna-1,4-dien-21-yl acetate) (Batch No. 161165) was purchased from Xianju Pharmaceutical Ltd. (Zhejiang, China). Adenine (Sigma-Aldrich, Lot#WXBB0585V) was purchased from Sigma (Shanghai, China). Creatinine (Crea), urea nitrogen (BUN), and total protein (TP) kits were purchased from Heguan Chemical Company (Beijing, China). The Bradford Protein Assay Kit (P0006) was purchased from Beyotime Biotechnology Company (Beijing, China). The qPCR detection kit (Lot# L20509) was purchased from Quanshijin BioChem Tech Ltd. (Beijing, China). Hematoxylin (MHS16-500ML, LOT#SLBK4909V) and Eosin Y (HT110116-500ML, LOT#J6425V) were purchased from Sigma (Shanghai, China). Masson's trichrome kits (Lot:0606A18) were purchased from Leagene Biotechnology Co., Ltd. (Beijing, China). A Bio-Rad electrophoresis unit and Bio-Rad ChemiDocXRS+ Gel Imaging System (Beijing, China), LEICA RM2235 paraffin slicer, and LEICA DM2500 Optical Microscope (Beijing, China) were used in this study.
Bradford protein assay kit p0006
Manufactured by Beyotime
Sourced in China
The Bradford Protein Assay Kit (P0006) is a colorimetric assay used for the quantitative determination of protein concentration. It is based on the principle of protein-dye binding, where the dye binds to the protein, resulting in a color change that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Dm2500 optical microscope, by Leica (1 mentions)
Rm2235 paraffin slicer, by Leica (1 mentions)
Tritonx 100 st795, by Beyotime (1 mentions)
Anti β actin aa128, by Beyotime (1 mentions)
Heparin sodium salt, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Trypsin, by GE Healthcare (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Chlorpromazine, by Merck Group (1 mentions)
Methyl β cyclodextrin mβcd, by Merck Group (1 mentions)
Hoechst 33258, by Beyotime (1 mentions)
Lyso tracker red dye, by Beyotime (1 mentions)
1 bromododecane, by Merck Group (1 mentions)
3 protocols using bradford protein assay kit p0006
1
Evaluation of Anemoside B4 Compound
2
Exosome Protein Characterization Protocol
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The primary antibodies anti‐CD63 (ab59479), anti‐MUC1 (ab70475), anti‐TRPC5 (ab63151) and anti‐Flotillin1 (ab133497) were purchased from Abcam Biotechnology (Cambridge, MA, USA), while the primary antibody anti‐β‐actin (AA128) was purchased from Beyotime Biotechnology (Nantong, Jiangsu Province, China). Proteinase K (ST532) and Triton X‐100 (ST795) were purchased from Beyotime Biotechnology. The secondary antibodies goat anti‐rabbit IgG (A0277) and goat anti‐mouse IgG (A0286) were purchased from Beyotime Biotechnology, while donkey anti‐mouse IgG (H+L) (Alexa Fluor 488, A‐21202) and goat anti‐rabbit IgG (H+L) (Alexa Fluor 568, A‐11011) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). RIPA (P0013B) and the Bradford Protein Assay Kit (P0006) were purchased from Beyotime Biotechnology. Fluorophore‐labeled donkey anti‐rabbit IgG antibody (A11374) and fluorophore‐labeled donkey anti‐mouse IgG antibody (A‐21202) and Total Exosome Isolation Kit (from plasma) (4484450) were purchased from Invitrogen (Camarillo, CA, USA). The syringe filter unit (0.22 μm) was purchased from Millipore (Darmstadt, Germany).
3
Cell Internalization Mechanisms of bPEI1800
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All reagents and solvents were used as received without further purification. bPEI1800 was purchased from Alfa Aesar (Ward Hill, MA, USA). 1-bromododecane, heparin sodium salt (150 USP [United States Pharmacopeia] units/mg), N-(2-hydroxyethyl) piperazine-N’-ethanesulfonic acid (HEPES), fluorescein isothiocyanate (FITC) isomer, ethidium bromide, chlorpromazine, methyl-β-cyclodextrin (M-β-CD), and sodium azide were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Trypsin, fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), and Roswell Park Memorial Institute (RPMI) 1640 medium were all provided by GE Healthcare UK Ltd (Little Chalfont, UK). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was from AMRESCO LLC (Solon, OH, USA). LysoTracker® Red dye, Hoechst 33258, apoptosis detection kit (C1062), and Bradford protein assay kit (P0006) were all from Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China). Luciferase assay kit (E1500) was from Promega Corporation (Fitchburg, WI, USA). Other chemicals and reagents were of analytical grade. Mouse mammary tumor cell line 4T1 was provided by the Department of Pathology in Institute of Medicinal Biotechnology in Peking Union Medical College (Beijing, People’s Republic of China) and cultured in RPMI 1640 containing 10% FBS.
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