Bax rabbit pab

Ouabain octahydrate (Cat #O3125) was purchased from Sigma Aldrich (USA) and was diluted to 10 mM in ultra-pure water and further used for cell treatment. Trypsin-EDTA, fetal bovine serum, penicillin-streptomycin, Hank’s Balanced Salt Solution, and trypan blue were purchased from Paneco (Russia). Neurobasal Medium, Supplement B-27, and 0.5 mM GlutaMax were purchased from Gibco (USA). MMLV RT Kit and TaqMan qPCR mix-HS were purchased from Evrogen (Russia). Primers were purchased from DNA-synthesis (Russia). GeneChip Rat Gene 2.0 ST Array; GeneChip WT PLUS Reagent Kit; GeneChipH WT Terminal Labeling Kit; and GeneChip Hybridization, Wash, and Stain Kit were purchased from Affymetrix (USA). Poly-L-ornithine, RIPA buffer, MgCl₂, trichloroacetic acid, KCl, reagents for SDS-PAGE, cocktails of protease and phosphatase inhibitors, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide were purchased from Sigma Aldrich. DC Protein Assay Kit was purchased from Bio-Rad (USA). Antibodies phospho-CREB (Ser133) (87G3) Rabbit mAb; β-Actin (D6A8) Rabbit mAb; Bcl-2 Rabbit pAb; Bax Rabbit pAb; and anti-rabbit IgG-HRP were purchased from Cell Signaling Technology (USA). SuperSignal West Pico Chemiluminescent Substrate, SuperSignal West Femto Chemiluminescent Substrate, DNase I, and PureLink RNA Mini Kit, SYTO13 were purchased from Thermo Fisher Scientific (USA).