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2 protocols using mn 18

1

Liver Microsome and Hepatocyte Metabolic Assay

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NNEI and MN-18 were obtained from Cayman Chemical (Ann Arbor, MI, USA). Rat and human liver microsomes and hepatocytes were obtained from XenoTech (Kansas City, KS, USA). Acetonitrile and formic acid were purchased from Fisher Scientific (Raleigh, NC, USA). Polysorbate 80 was purchased from Fisher (Pittsburgh, PA, USA), and saline from Patterson Vet Supply (Devens, MA, USA). All chemicals and solvents were at least ACS or high-performance liquid chromatography grade, respectively.
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2

Analytical Characterization of Synthetic Cannabinoids

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Synthetic cannabinoids CUMYL-PICA, 5F-CUMYL-PICA, AMB-FUBINACA, MDMB-FUBINACA, NNEI, and MN-18 were obtained from Cayman Chemical (Ann Arbor, MI, USA); acetonitrile and methanol from Fisher (Pittsburgh, PA, USA); helium carrier gas from Airgas (Durham, NC, USA); boric acid, 2,3-naphthalenedicarboxaldehyde (NDA), potassium cyanide (and K13C15N internal standard), taurine (2-aminoethanesulfonic acid), and sodium hydroxide were obtained from Sigma-Aldrich (Castle Hill, NSW, Australia). All chemicals and solvents were at least ACS or high-performance liquid chromatography grade, respectively.
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