Rota count 8

Manufactured by Columbus Instruments

Sourced in United States

The Rota-Count 8 is a rotational activity monitoring system designed for laboratory use. It tracks and records the rotational activity of up to 8 individual subjects simultaneously. The device provides objective data on the activity levels of the subjects over time.

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Lab products found in correlation

Apomorphine, by Bio-Techne (2 mentions) D amphetamine, by Merck Group (1 mentions) Normal saline, by Merck Group (1 mentions)

6 protocols using rota count 8

Amphetamine-Induced Rotational Behavior

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Rotational behavior induced by amphetamine was tested 4 weeks after the 6-OHDA injection to assess the extent of the dopaminergic lesion. Rotations were measured using an automated rotometer (Rota-count 8, Columbus Instruments, Columbus, OH, USA). Left and right full body turns induced by 2.5 mg/kg (in saline) amphetamine intraperitoneal injection were counted over a 90 min period. Rats showing rotations higher than 6 net full turns per minute towards the lesioned side were identified as maximally lesioned rats (i.e., showing more than 90% depletion of striatal dopaminergic terminals) [40 (link)] and were included in the study.

Lopez-Lopez A., Valenzuela R., Rodriguez-Perez A.I., Guerra M.J., Labandeira-Garcia J.L, & Muñoz A. (2023). Interactions between Angiotensin Type-1 Antagonists, Statins, and ROCK Inhibitors in a Rat Model of L-DOPA-Induced Dyskinesia. Antioxidants, 12(7), 1454.

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Apomorphine-Induced Rotation Assay for Parkinson's Model

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The apomorphine-induced rotation test was applied to validate the PD model, since the number of asymmetric rotations correlates with nigral degeneration, as previously demonstrated [17 (link)]. Rotational asymmetric behavior was evaluated using an automatic rotometer system (Rota-Count 8, Columbus Instruments, Columbus, OH, USA) 7 days after striatal saline or 6-OHDA injection. Animals were injected with the dopamine agonist apomorphine (1 mg/kg, s.c., Tocris Bioscience, BZ, Bristol, UK) dissolved in 0.9% saline and were evaluated over 30 min, as previously described [57 (link)]. The criterion for rotation was a 180° turn toward the side contralateral to the lesion. To reduce stress, the rats were habituated for 30 min one day before the rotational test. The saline rats were also evaluated.

Campos A.C., Berzuíno M.B., Barbosa G.R., Freire H.M., Lopes P.S., Assis D.V., Fonoff E.T, & Pagano R.L. (2021). Motor Cortex Stimulation Reversed Hypernociception, Increased Serotonin in Raphe Neurons, and Caused Inhibition of Spinal Astrocytes in a Parkinson’s Disease Rat Model. Cells, 10(5), 1158.

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Assessing Amphetamine-Induced Rotational Behavior

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Amphetamine-induced rotational behavior, a measure of the loss of DA after a unilateral lesion (Przedborski et al., 1995 (link)), was assessed four weeks after 6-OHDA infusion. Animals were jacketed and tethered to a sensor attached to the top of the stainless steel rotameter bowl (Rota Count 8; Columbus Instruments, Columbus, OH). Once securely in the bowls, animals received D-amphetamine-sulfate (3 mg/kg free base, i.p.) and turning was monitored for 1 h.

Castro S.L., Tapias V., Gathagan R., Emes A., Brandon T.E, & Smith A.D. (2022). Blueberry juice augments exercise-induced neuroprotection in a Parkinson’s disease model through modulation of GDNF levels. IBRO Neuroscience Reports, 12, 217-227.

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Evaluating Lesion and Graft Survival in Parkinson's Rat Model

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The efficacy of the lesion and graft survival were evaluated one month after the lesion and 1, 2, or 3 months after grafting in the rats included within the long-term survival experiments (i.e., 3 months p.g.) by the rotometer test [46 (link)]. The efficacy of the lesion and graft survival were also confirmed by subsequent TH (as a dopaminergic marker) IHC of the SN and striatum at the end of the experiments (see below). Drug-induced rotation was tested in a bank of eight automated rotometer bowls (Rota-count 8, Columbus Instruments, Columbus, OH, USA). Turning behavior was monitored for 90 min after injection of D-amphetamine (5 mg/kg in saline; intraperitoneally; Sigma-Aldrich). Rats showing at least 7 net full turns towards the lesioned side were identified as maximally lesioned animals and were included in the study.

Rodríguez-Pérez A.I., Garrido-Gil P., García-Garrote M., Muñoz A., Parga J.A., Labandeira-García J.L, & Rodríguez-Pallares J. (2024). Non-HLA angiotensin-type-1 receptor autoantibodies mediate the long-term loss of grafted neurons in Parkinson’s disease models. Stem Cell Research & Therapy, 15, 138.

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Methamphetamine-Induced Rotation in Mice

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Mice were assessed for unilateral loss of dopaminergic neurons that could induce rotation behavior at 14- and 28-days post-injection using an automated rotation monitoring system (Rota-Count 8; Columbus Instruments, Columbus, OH). The system records full and partial turning motion of small laboratory animals wearing a harness to which a light and flexible tether has been affixed at one end and to a rotary encoder at the opposite end to monitor the angular direction of the subject. The system monitors both clockwise and counter-clockwise turning behavior, which can be measured using the accompanying software. After harness placement, mice were injected with 2.5 mg/kg of methamphetamine (575 μg/mL in the normal saline; Sigma–Aldrich, St. Louis, MO, USA) intraperitoneally to induce rotation and the number of complete clockwise (ipsiversive) and counterclockwise (contraversive) rotations were measured over a 60-minute period.

Gupta R.V., Santiago-Lopez A.J., Berglund K., Gross R.E, & Gutekunst C.A. (2020). In vivo assessment of cell death and nigrostriatal pathway integrity following continuous expression of C3 transferase. Neuroscience, 442, 183-192.

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Apomorphine-Induced Rotation Model for PD

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To validate the PD model, animals were subjected to apomorphine-induced rotation seven days after the surgical procedure, as previously described [47 (link)]. Briefly, animals were injected with a dopaminergic agonist (apomorphine, 1 mg/kg, subcutaneous (s.c.), Tocris Bioscience, Ellisville, MI, USA) dissolved in 0.9% saline, and the number of rotations was recorded over 30 min using an automatic rotometer system (Rota-Count 8, Columbus Instruments, Columbus, OH, USA). No animal injected with 6-OHDA showed asymmetric rotational behavior.

Pinheiro Campos A.C., Martinez R.C., Auada A.V., Lebrun I., Fonoff E.T., Hamani C, & Pagano R.L. (2022). Effect of Subthalamic Stimulation and Electrode Implantation in the Striatal Microenvironment in a Parkinson’s Disease Rat Model. International Journal of Molecular Sciences, 23(20), 12116.

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