45u xbal

Manufactured by Takara Bio

The 45U Xbal is a laboratory equipment designed for use in DNA manipulation and analysis. It functions as a restriction enzyme that specifically recognizes and cleaves the DNA sequence 5'-TCTAGA-3'.

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Lab products found in correlation

Chef dr 3 apparatus, by Bio-Rad (2 mentions)

2 protocols using 45u xbal

Genetic Relatedness of K. pneumoniae Isolates

Cited 1 time

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We performed PFGE to check whether there is the presence of any clonal transmission within the hospital and MLST was applied to assess the genetic relatedness of the identified isolates. Post extraction, DNA was digested with 45U Xbal (Takara Biotech) for 2 h at 37 °C. We used CHEFDRIII apparatus (Bio-Rad Laboratories, Hercules, CA, USA) to perform PFGE for K. pneumoniae isolates as per earlier described [13 (link)]. Amplified fragments of seven housekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) were sequenced to MLST analysis and MLST website MLST website-http://www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae was referred to assign the STs.

Patil S., Chen X, & Wen F. (2019). Exploring the phenotype and genotype of multi-drug resistant Klebsiella pneumoniae harbouring blaCTX-M group extended-spectrum β-lactamases recovered from paediatric clinical cases in Shenzhen, China. Annals of Clinical Microbiology and Antimicrobials, 18, 32.

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PFGE and MLST of K. pneumoniae

Cited 2 times

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Post-extraction DNA was digested with 45U Xbal (Takara Biotech) for 2 hours at 37°C. We used CHEFDRIII apparatus (Bio-Rad Laboratories, Hercules, CA, USA) to perform PFGE for K. pneumoniae isolates as previously described.23 (link) PCR assay was performed to amplify internal portions of the seven housekeeping genes of K. pneumoniae (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) with specific primers.24 (link) Amplified products were sequenced from private enterprise (Sangon Biotech-Shanghai, China

http://www.pasteur.fr/recherche/genopole/PF8/mlst/Kpneumoniae

) and were referred to assign sequence types (STs).

Patil S., Chen H., Guo C., Zhang X., Ren P.G., Francisco N.M, & Wen F. (2021). Emergence of Klebsiella pneumoniae ST307 Co-Producing CTX-M with SHV and KPC from Paediatric Patients at Shenzhen Children’s Hospital, China. Infection and Drug Resistance, 14, 3581-3588.

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