Hs crp assay kit

At baseline, 2 weeks of dyslipidemia production and 2 weeks of medical therapy, fasting venous blood was drawn for laboratory examination. Lipid profiles including triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and LDL-C, fasting blood glucose (FBG), liver enzymes including alanine aminotransferase (ALT) and aspartase aminotransferase (AST) were detected by Automatic Biochemistry Analyzer (Beckman coulter UniCel DxC 800 Synchron). Parameters of endothelial function including nitric oxide (NO) production (ELISA kit, Nanjing Jiancheng Bioengineering Institute) and serum level of asymmetric dimethylarginine (ADMA, ELISA kit, Shanghai Ying-gong Industrial Company) were assessed in accordance to the manufacture’s introduction. Serum levels of high sensitive C-reactive protein (Hs-CRP Assay Kit, Immune-turbidimetry method, Nanjing Jiancheng Bioengineering Institute) and malondialdehyde (MDA Assay Kit, TBA method, Nanjing Jiancheng Bioengineering Institute) were measured to evaluate the changes of systemic inflammation and oxidation over time. Serum ROCK activity was detected by enzyme-linking immune-absorbent assay (ELISA kit, Yuping BioMedical Company, Shanghai, China).

Patients in each group were extracted vein blood 5 mL at 4 times: before surgery (T0), during surgery (T1), 2 hours after surgery (T2), and 24 hours after surgery (T3). Serum levels of high sensitive C-reactive protein was detected by enzyme-linking immune-absorbent assay (Hs-CRP Assay Kit, Nanjing Jiancheng Bioengineering Institute, China). The levels of blood glucose and leukocyte count were measured respectively by blood glucose meter and automatic blood cell counting equipment.