Cell apoptosis assays were performed using the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences) by flow cytometry analysis (FCA). Cell cycle analysis was detected by the Cell cycle staining solution kit (MultiSciences, China). The percentages of the cells in G0–G1, S, and G2/M phases were counted and compared.
Cell cycle staining solution kit
Manufactured by MultiSciences Biotech
Sourced in China
The Cell Cycle Staining Solution Kit is a laboratory product developed by MultiSciences Biotech. The kit provides the necessary reagents and protocols to stain and analyze cell cycle stages using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cell cycle staining solution kit
1
Apoptosis and Cell Cycle Analysis
2
Cell Cycle and Apoptosis Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cycle was analyzed with a flow cytometry assay.
After transfection for 48 h, approximately 1 × 10 6 cells were collected and prepared using a cell cycle staining solution kit (MultiSciences Biotech, Hangzhou, China) according to the product's instructions. For the cell apoptosis assay, cells were resuspended in 300 µL of 1 × binding buffer, and HepG2 and SMMS-7721 cells were stained with Annexin V-APC and 7-Aminoactinomycin D (7-AAD) according to the manufacturer's instructions (MultiSciences Biotech), and incubated at room temperature for 15 min in the dark. Next, the cell suspension was load on a Fluorescence Activated Cell Sorting (FACS) Calibur flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA). Finally, data analysis was performed using FCS Express version 3 software (DeNovo Software, Los Angeles, CA, USA), and the percentages of cells in G0/G1, S, G2/M phase and apoptosis rate were counted and compared.
After transfection for 48 h, approximately 1 × 10 6 cells were collected and prepared using a cell cycle staining solution kit (MultiSciences Biotech, Hangzhou, China) according to the product's instructions. For the cell apoptosis assay, cells were resuspended in 300 µL of 1 × binding buffer, and HepG2 and SMMS-7721 cells were stained with Annexin V-APC and 7-Aminoactinomycin D (7-AAD) according to the manufacturer's instructions (MultiSciences Biotech), and incubated at room temperature for 15 min in the dark. Next, the cell suspension was load on a Fluorescence Activated Cell Sorting (FACS) Calibur flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA). Finally, data analysis was performed using FCS Express version 3 software (DeNovo Software, Los Angeles, CA, USA), and the percentages of cells in G0/G1, S, G2/M phase and apoptosis rate were counted and compared.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!