Serum samples were assayed for HHIP (Meimian Biotechnology, Yancheng, Jiangsu, China) and adipoq (sk00010-01, Aviscerabio science Inc., USA) using the ELISA kits. Kits for HHIP had intra- and inter-assay coefficients of variation (CV) less than 10% and 8%, respectively. The minimum detection level for human HHIP is 0.1 ng/mL, with a detection range of 0.625–20 ng/mL. The method had high sensitivity and good specificity for human HHIP without obvious cross-reactivity or interference. Intra- and inter-assay CV for adipoq were < 9% and < 11%, respectively. Serum Adipoq was determined as previously reported [31 (link)].
Sk00010 01
Manufactured by Aviscera Bioscience
Sourced in United States, China
The Sk00010-01 is a laboratory instrument designed for basic spectroscopic analysis. It provides a reliable means of measuring the absorbance or transmittance of light through a sample across a range of wavelengths. This device is capable of performing essential spectroscopic measurements required in various scientific applications.
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Lab products found in correlation
5 protocols using sk00010 01
1
Quantifying Circulating HHIP and Adipoq
2
Measurement of Circulating GPHB5 Levels
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We used an ELISA kit to measure circulating GPHB5 levels according to the manufacturer’s instructions (Kete Biotechnology Co., Ltd, Jiangshu, China). This kit has high sensitivity and specificity for the measurement of GPHB5 without obvious cross-reactivity. The intra- and interassay coefficients of variation (CVs) were< 10% and< 8%, respectively. As previously published (23 (link)), serum adiponectin (Adipoq) concentrations were measured by an ELISA (sk00010-01, Aviscerabioscience Inc., USA), with intra- and inter-assay CVs of 4% - 8% and 8% - 12%, respectively.
3
Serum CTRP7, CTRP15, and APN Evaluation
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The serum concentrations of CTRP7 and CTRP15 were determined using ELISA kits (sk00396-09 and sk00393-19, Aviscera Bioscience Inc., USA) according to the manufacturer's instructions. In CTRP7 assessment, both intra- and inter-assay variations were meticulously contained, demonstrating coefficients of variation (CV) less than 5 % and 10 %, respectively. For CTRP15 evaluation, the corresponding intra- and inter-assay CVs were below 8 % and 12 %, respectively. APN serum concentrations were determined using an ELISA kit (sk00010-01, Aviscera Bioscience Inc., USA) with intra- and inter-assay CVs of 4–8% and 8–12 %, respectively. The composite index, log10 (CTRP7+CTRP15), was calculated based on the serum concentrations of CTRP7 and CTRP15.
4
Serum HHIP and Adipoq Quantification
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Serum HHIP and Adipoq concentrations were measured using ELISA kits according to the manufacturer’s instructions (Kete Biotechnology Co., Ltd, Jiangsu, China and sk00010-01, Aviscerabioscience Inc., USA). These two kits assessed HHIP and Adipoq with high sensitivity and specificity, without obvious cross-reactivity. The intra- and interassay coefficients of variation (CVs) for HHIP were <10% and <8%, whereas they were <8% and <12% for Adipoq, respectively.
5
Quantifying Serum CTRP7 and ADIPOQ
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Serum CTRP7 concentration was determined by an ELISA Kit according to the manufacturer's procedure (sk00396-09, Aviscerabio science Inc., USA). This ELISA Kit has high sensitivity and specificity for the detection of human CTRP7 without obvious cross-reaction and interference. The intra- and interassay coefficients of variation (CV) were 4-6% and 8-10%, respectively. As previously reported [17 (link)], serum ADIPOQ concentrations were measured by an ELISA (sk00010-01, Aviscerabio science Inc., USA), with intra- and interassay CV of 4-8% and 8-12%, respectively.
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