All cells were maintained in 5% CO2 at 37°C. THP-1 Blue cells (human monocytic leukemia line with an NF-κB–inducible reporter (Invivogen) and RAW264.7 cells carrying the NF-κB luciferase plasmid (IMGENEX, USA) were maintained in RPMI 1640 medium (Invitrogen) and Iscove's modified Dulbecco's medium (IMDM, Invitrogen), respectively. The media were supplemented with 10% heat-inactivated FBS (endotoxin-free Certified FBS; Invitrogen), 2% penicillin/streptomycin, 2 mM L-glutamine, and 10 mM HEPES (all from Gibco). The cells were seeded in T-75 tissue culture flasks (Falcon, USA) and used between passages 2 and 3.The THP-1 and RAW264.7 cell lines were extensively validated by the Invivogen and IMAGENEX respectively. For quality control, we have tested the cells for mycoplasma with PlasmoTest™ kit (Invivogen).
Nf κb inducible reporter
Manufactured by InvivoGen
Sourced in United States
The NF-κB–inducible reporter is a laboratory equipment product designed to monitor the activity of the NF-κB transcription factor. It functions by expressing a reporter gene, such as luciferase or fluorescent protein, under the control of an NF-κB-responsive promoter. This allows for the quantification of NF-κB activation in response to various stimuli in cell-based assays.
Automatically generated - may contain errors
Lab products found in correlation
Thp1 blue cells, by InvivoGen (2 mentions)
Endotoxin free certified fbs, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Hepes, by Thermo Fisher Scientific (2 mentions)
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (2 mentions)
Plasmotest kit, by InvivoGen (2 mentions)
T 75 tissue culture flasks, by BD (2 mentions)
2 protocols using nf κb inducible reporter
1
NF-κB Activation in Monocytic Cell Lines
2
NF-κB Activation in Monocytic Cell Lines
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