Gas003

Manufactured by Thermo Fisher Scientific

The GAS003 is a laboratory gas analyzer designed to measure the concentration of various gases. It utilizes advanced sensor technology to accurately detect and quantify the levels of specific gases within a sample. The core function of the GAS003 is to provide precise and reliable gas analysis for a variety of applications.

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Lab products found in correlation

Facsverse, by BD (1 mentions) Sca 1, by BD (1 mentions) Ki67 apc, by BD (1 mentions) Cd150, by BD (1 mentions) Facsaria, by BD (1 mentions) Streptavidin percp cy5, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

2 protocols using gas003

Vascular Smooth Muscle Cell Characterization

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VSMCs were harvested by centrifugation, washed, and resuspended in PBS. Cells were then fixed with fixation medium (GAS003, Invitrogen Life Technologies) for 15 min at room temperature before adding primary antibody. After washing with PBS, cells were incubated with permeabilization medium (GAS003, Invitrogen Life Technologies) and primary antibody or the corresponding isotype-matched IgG control (BD Biosciences) for 30 min in the dark at room temperature. Cells stained for BMP2, Runx2 or osteopontin were washed twice and incubated with FITC-conjugated anti-mouse secondary antibody (554001, BD Biosciences) or FITC-conjugated anti-rabbit secondary antibody (sc-2090, Santa Cruz). The cells were subjected to flow cytometric analysis using FACSVerse (BD Biosciences), and data were analyzed with FlowJo software.

Feng W., Zhang K., Liu Y., Chen J., Cai Q., Zhang Y., Wang M., Wang J, & Huang H. (2016). Apocynin attenuates angiotensin II-induced vascular smooth muscle cells osteogenic switching via suppressing extracellular signal-regulated kinase 1/2. Oncotarget, 7(50), 83588-83600.

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Multiparametric Flow Cytometry

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CD117 (c-kit; APC-Cy7 or APC-eFluor780), sca1 (PE-Cy7), CD150 (APC), CD48 (PE), Lineage (CD3, Ter119, B220, Gr1; Biotin), streptavidin-PerCP-Cy5.5, CD4 (PE-Cy7), CD8a (PE), CD45.1 (APC-Cy7) and CD45.2 (FITC) from antibodies from BD. DAPI (D1306, Invitrogen) was used for viability. Cell-cycle was analyzed with Ki67-APC (558615, BD Pharmingen) in permeabilizing buffer (GAS-003, Invitrogen) and DAPI. Apoptosis was analyzed with AnnexinV/DAPI-kit (BD) FACS was performed on LSRII (BD), sorting on FACSAria (BD) and data analyzed with FlowJo v.10 (TreeStar, Inc).

Gekas C., D'Altri T., Aligué R., González J., Espinosa L, & Bigas A. (2016). β-Catenin is required for T-cell leukemia initiation and MYC transcription downstream of Notch1. Leukemia, 30(10).

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