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Amicon ultra centrifugal filter units amicon ultra 15 10 000 mwco

Manufactured by Merck Group
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The Amicon Ultra Centrifugal Filter Units are a type of lab equipment designed for sample concentration and buffer exchange. The units feature a regenerated cellulose membrane with a molecular weight cutoff (MWCO) of 10,000 Daltons. The core function of these filters is to separate and concentrate macromolecules such as proteins, peptides, or nucleic acids from sample solutions by means of centrifugation.

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Lab products found in correlation

Ni nta affinity resin, by Qiagen (2 mentions) Nabh3t solution, by American Radiolabeled Chemicals (2 mentions) Isopropylthio d galactopyranoside iptg, by Thermo Fisher Scientific (2 mentions)

2 protocols using amicon ultra centrifugal filter units amicon ultra 15 10 000 mwco

1

Purification and Radiolabeling of SCO7700

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents and solvents were purchased from Sigma-Aldrich or Fisher Scientific, were of the highest quality available, and were used without further purification. NaBH3T solution (6 μmol, 80 Ci/mmol specific activity, 500 mCi total in 1 mL of 0.01 N NaOH) was purchased from American Radiolabeled Chemicals. Isopropylthio-D-galactopyranoside (IPTG) was purchased from Invitrogen. Ni-NTA affinity resin was from Qiagen. Amicon Ultra Centrifugal Filter Units (Amicon Ultra-15 10,000 MWCO) were purchased from Millipore. Purified SCO7700 protein was overexpressed and purified as previously described.7 (link)
Chou W.K., Gould C.A, & Cane D.E. (2017). Incubation of 2-Methylisoborneol Synthase with the Intermediate Analogue 2-Methylneryl Diphosphate. The Journal of antibiotics, 70(5), 625-631.
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2

Purification and Radiolabeling of SCO7700

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents and solvents were purchased from Sigma-Aldrich or Fisher Scientific, were of the highest quality available, and were used without further purification. NaBH3T solution (6 μmol, 80 Ci/mmol specific activity, 500 mCi total in 1 mL of 0.01 N NaOH) was purchased from American Radiolabeled Chemicals. Isopropylthio-D-galactopyranoside (IPTG) was purchased from Invitrogen. Ni-NTA affinity resin was from Qiagen. Amicon Ultra Centrifugal Filter Units (Amicon Ultra-15 10,000 MWCO) were purchased from Millipore. Purified SCO7700 protein was overexpressed and purified as previously described.7 (link)
Chou W.K., Gould C.A, & Cane D.E. (2017). Incubation of 2-Methylisoborneol Synthase with the Intermediate Analogue 2-Methylneryl Diphosphate. The Journal of antibiotics, 70(5), 625-631.
+ Open protocol
+ Expand

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