Carbon 14 [14C]-labeled compounds are often used to determine drug release amounts. The release of metformin was assessed following our previous method [18 (link), 19 (link)]. Briefly, 10, 30, and 50 μg of metformin (containing 1/10 [14C]-metformin) were dissolved in 15% chitosan to form the metformin + chitosan liquid. The powder and liquid portions were mixed under sterile conditions by hand spatulation. [14C] metformin-loaded scaffold was placed in 24-well plates containing 1 mL of PBS in an incubator at 37°C. At each time period, the microsphere suspension was centrifuged, and the PBS without microspheres was collected for [14C]-metformin concentration analysis. 100 μL PBS was transferred to the scintillation tube containing 3 mL Biodegradable Counting Cocktail buffer (Fisher Scientific Inc., Pittsburgh, PA). Radioactivity was counted by a multipurpose scintillation counter (Beckman LS6500 Counter, Brea, CA).
Biodegradable counting cocktail buffer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Biodegradable Counting Cocktail buffer is a laboratory product designed to facilitate the counting of radioactive samples. It is a non-hazardous, biodegradable solution that can be used in conjunction with radioactive detection equipment.
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2 protocols using biodegradable counting cocktail buffer
1
Metformin Release Quantification via 14C Labeling
2
Quantifying Radiolabeled Drug Release
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Carbon14[ 14 C]-labelled materials are used to evaluate the drug release profiles. The release of SB431542 was assessed following a previous study 39) . Briefly, CPCSM disks were cultured in 24-well plates with 1 mL of cell culture medium in an incubator, and 100 μL cell culture medium was withdrawn at each time interval. CPCSM (SM containing 1/10 [ 14 C]-adenosine) scaffold were cultured in 24-well plates with 1 mL of cell culture medium in an incubator. The released solution was analyzed at various time points (1, 3, 7, 14, and 21 days). At every pre-determined time point, centrifugation was performed for the microsphere suspension. Then the PBS without microspheres was used for measuring the [ 14 C]-SB431542 concentration. Then, PBS (100 μL) was withdrawn at each time interval and transferred to a scintillation tube containing 3 mL Biodegradable Counting Cocktail buffer (Fisher Scientific, Pittsburgh, PA, USA). The amount of adenosine released from the CPC scaffold was quantified via counting the radioactivity using a scintillation method (Beckman LS6500 Counter, Brea, CA, USA).
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