Hepatic levels of malondialdehyde (MDA), a marker of lipid peroxidation, were determined by a thiobarbituric acid method using MDA kit (Biodiagnostic, Egypt). The formed colored thiobarbituric acid reactive product was measured spectrophotometrically at 534 nm (Gavino et al., 1981 (link)). Hepatic glutathione (GSH) levels were measured by GSH kit (Biodiagnostic, Egypt) based on the reduction of 5,5‘dithiobis (2-nitrobenzoic acid) by GSH. The absorbance of colored product was measured at 405 nm (Beutler, 1963 (link)). Aliquots of liver homogenate were used for determination of nuclear Nrf-2, GSK-3β and PI3K levels using rat-specific ELISA kits provided by Wuhan Fine Biological Technology Co., China (catalog no.: ER911, ER0124 and ER1531, respectively). Hepatic levels of HO-1 were estimated by rat-specific ELISA kit supplied by Elabscience Biotechnology Inc., USA (catalog no.: E-EL-R0864) while hepatic rat pAkt concentrations were measured by Cusabio® ELISA kit (China). The Pierce™ bicinchoninic acid (BCA) assay kit supplied by ThermoFisher Scientific, USA (catalog no.: 23227) was used to determine total protein content (Smith et al., 1985 (link)). Tissue parameters were expressed per mg of protein.
Mda kit
Manufactured by Biodiagnostic
Sourced in Egypt
The MDA kit is a laboratory equipment used for the detection and quantification of malondialdehyde (MDA), a biomarker for oxidative stress. The kit provides a colorimetric assay for the measurement of MDA levels in various biological samples.
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Lab products found in correlation
4 protocols using mda kit
1
Hepatic Oxidative Stress Biomarkers Quantification
2
Oxidative Stress Biomarkers in Testis
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Total antioxidant capacities (TAC) together with the level of peroxide, that reflect total oxidative stress status (TOS), were measured in serum using a colorimetric assay by (Biodiagnostics, Tahreer St, Dokki, Giza, Egypt) with TAC kit (Cat. No. TA 25) Peroxide kit (Cat. No. HP25).
Levels of oxidative stress markers; malondialdehyde (MDA) (marker for lipid per-oxidation) and activities of antioxidant enzymes catalase, glutathione peroxidase in testicular tissue homogenate were measured according to the manufacturer’s instructions using a colorimetric assay by (Biodiagnostics, Tahreer St, Dokki, Giza, Egypt) with the following kits: MDA kit (Cat. No. MD 2529), catalase (Cat. No. CA 25 17), glutathione peroxidase (Cat. No. GP 2524).
Levels of oxidative stress markers; malondialdehyde (MDA) (marker for lipid per-oxidation) and activities of antioxidant enzymes catalase, glutathione peroxidase in testicular tissue homogenate were measured according to the manufacturer’s instructions using a colorimetric assay by (Biodiagnostics, Tahreer St, Dokki, Giza, Egypt) with the following kits: MDA kit (Cat. No. MD 2529), catalase (Cat. No. CA 25 17), glutathione peroxidase (Cat. No. GP 2524).
3
Lipid Peroxide Colorimetric Quantification
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The lipid peroxide content was measured colorimetrically at a wavelength of 534 nm using an MDA kit (Bio-diagnostic, Cairo, Egypt; Cat #: MD2529) and processed according to the manufacturer’s instructions.
4
Quantification of Lipid Peroxidation Biomarker MDA
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Considered as a biomarker of lipid peroxidation, malondialdehyde (MDA) was quantified using the MDA kit (Cat. no: MD 25 29) from Bio-diagnostic Co.,Ltd (12) following the manufacture instructions. The activity was measured in cell lysate and the absorbance was assessed at 534 nm.
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