Andbovine serum albumin bsa

All of the
reagents and solvents used in this work were of analytical grade and
allyl 4-hydroxybenzoate, m-chloroperoxybenzoic acid
(m-CPBA, 85%), Na₂HPO₄, NaH₂PO₄, dimethylformamide (DMF), dichloromethane,
and ethanol were purchased from Tokyo Chemical Industry (Shanghai).
Poly(vinylbenzyl chloride) (PVBC, a 60/40 mixture of 3- and 4-isomers
with average molecular weight M_n ca. 55 000
and M_w ca. 100 000), human immunoglobulin
G (IgG), goat anti-human immunoglobulin G antibody (anti-IgG), and
bovine serum albumin (BSA) were obtained from Sigma-Aldrich. Amino-modified
multiwalled carbon nanotubes (−NH₂ content: 0.45
wt %, length: ∼50 um, outer diameter: 8–15 nm) were
purchased from Nanjing XFNANO Materials Tech Co., Ltd. Double distilled
water was used in this work. Phosphate-buffered saline (PBS, 0.1 M,
pH 7.2) was prepared using Na₂HPO₄ and NaH₂PO₄. Three human serum samples were donated by
the Brain Hospital of Hunan Province, China.

Lactoferrin was purchased from Monojo (Amman, Jordan), colloidal
gold nanoparticles (30 nm) and anti-norovirus specific antibodies
GI Mab (clone NG28) and anti-norovirus GII Mab (clone NP8) were purchased
from Atlas Medical Company (Amman, Jordan). Sodium periodate (NalO₄), sulfuric acid, phosphate buffer saline (PBS) buffer, and
bovine serum albumin (BSA) were purchased from Sigma-Aldrich. Cotton
buds were purchased from the local market.