Human paraffin-embedded tissue array (Tissue Microarray, TMA) contained multiple benign tumor (nevus) and metastatic melanoma specimens. Immunohistochemical staining was performed at the University of Michigan Rogel Cancer Center Histology core on the DAKO Autostainer (Agilent, Carpinteria, CA) using Envision Flex+ and diaminobenzadine (DAB) as the chromogen. De-paraffinized sections were labeled with anti-TRPML1 specific antibody at 1:250 for 30 mins at room temperature. Heat induced epitope retrieval using Dako Envision Flex TRS, Low pH was used prior to staining. Appropriate negative (no primary antibody) and positive controls (Titer TMA 4A) were stained in parallel with each set of slides studied. Images were obtained by Nikon’s Eclipse E800 microscope.
Envision flex trs
Manufactured by Agilent Technologies
The Envision Flex TRS is a liquid chromatography system designed for routine analysis and quantification of small molecules. It features a modular architecture, allowing for customization to meet specific analytical requirements. The system provides reliable and reproducible results through precise fluidic control and advanced detection capabilities.
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2 protocols using envision flex trs
1
Immunohistochemical Analysis of TRPML1 in Melanoma
2
Immunohistochemical Analysis of TRPML1 in Melanoma
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Human paraffin-embedded tissue array (Tissue Microarray, TMA) contained multiple benign tumor (nevus) and metastatic melanoma specimens. Immunohistochemical staining was performed at the University of Michigan Rogel Cancer Center Histology core on the DAKO Autostainer (Agilent, Carpinteria, CA) using Envision Flex+ and diaminobenzadine (DAB) as the chromogen. De-paraffinized sections were labeled with anti-TRPML1 specific antibody at 1:250 for 30 mins at room temperature. Heat induced epitope retrieval using Dako Envision Flex TRS, Low pH was used prior to staining. Appropriate negative (no primary antibody) and positive controls (Titer TMA 4A) were stained in parallel with each set of slides studied. Images were obtained by Nikon’s Eclipse E800 microscope.
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