Lapatinib

All cell culture media and supplements were purchased from Sigma-Aldrich (UK). Monensin, resazurin and MG132 were from Sigma-Aldrich, and plasticware was from Sarstedt. Primaquine, lapatinib, enzalutamide and MTT were purchased from Carbosynth, UK. BafA1 was obtained from Merck.
Antibodies specific for ER-a (#8644), AR (#5153) and HER3 (#12708) were from Cell Signalling Technology.
Mouse monoclonal anti-alpha tubulin (T5168) is from Sigma-Aldrich. 680RD-conjugated goat anti-Mouse (C80619-5) and 800CW-conjugated goat anti-Rabbit (C80426-08) secondary antibodies were from LI-COR Biosciences.

Furosemide (≥ 98% (HPLC), (±)-metoprolol (+)-tartrate salt (≥ 98% (titration)), 1,1dimethylbiguanide hydrochloride (metformin hydrochloride, 97%), danazol (≥ 98%), itraconazole (≥ 98% (TLC)), propafenone hydrochloride (≥ 98% (HPLC)), celecoxib (≥ 98% (HPLC)), azithromycin (≥ 95% (NT)) and atovaquone (≥ 98% (HPLC)) were all purchased from Sigma-Aldrich, UK. Nifedipine (98 to 102% (on dried substance)), paracetamol (97.5% min. (HPLC)), atorvastatin calcium (pharmaceutical secondary standard; traceable to USP, PhEur), atenolol (≥ 98% (TLC)) and ketoconazole (inclusive between 98%) were all purchased from Fisher Scientific, UK. Pravastatin sodium (≥ 98%) and lapatinib (≥ 99% (HPLC)) were purchased from Carbosynth, UK. MK-C1, MK-C2, MK-C3 and MK-C4 were provided by Merck & Co, INC, US. Sodium chloride, sodium hydroxide, sodium dodecyl sulphate, sodium acetate trihydrate, dipotassium hydrogen orthophosphate, potassium dihydrogen phosphate, ammonium acetate, hydrochloric acid 37% glacial acetic acid ≥ 99% and trichloroacetic acid 10% w/v were all purchased from Fisher Scientific, UK. HPLC grade methanol, ethanol, acetonitrile, acetone, trifluoroacetic acid (≥ 99%) were all purchased from Sigma-Aldrich, UK.
3.6% fat UHT milk was commercially purchased (Sainsbury's, UK).