Mouse crg 2 cxcl10 elisa kit

The protein expression of IFN-γ-inducible chemokines in treated mouse skin biopsies were quantified by using mouse CXCL9/MIG DuoSet ELISA kit (R&D System, #DY492-05) combined with DuoSet Ancillary Reagent Kit 2 (R&D System, #DY008), and mouse CRG-2 (CXCL10) ELISA kit (Invitrogen, #EMCXCL10) according to the manufacturer’s instructions. Optical densities were measured using a Tecan M1000 microplate reader, and the absorbance at 540 nm was used to calculate CXCL9 levels using a standard curve derived from known concentrations of the standards. For CXCL10, optical densities at 450 nm were measured on a SpectraMax M5 microplate reader.

Cell studies: Human IFN-γ R1 ELISA kit (Invitrogen, #EH249RB) and mouse IFN-γ R1 ELISA kit (Invitrogen, #EM40RB) were used according to manufacturer’s instructions. Human HeLa, SH-SH5Y, and mouse N2a cells were seeded in a 24-well plate and treated with 500 μL siRNA at 1.5 μM for 72 h. Cells were lysed with 700 μL/well RIPA buffer with proteinase inhibitor cocktail (Roche, #11836153001). Total protein levels were quantified by Bradford assay kit (ThermoFisher Scientific, #23236).
Animal studies: Mouse CXCL9 DuoSet ELISA kit (R&D System, #DY492-05) and DuoSet Ancillary Reagent Kit 2 (R&D System, #DY008) were used according to manufacturer’s instructions. Optical densities were measured with a Tecan M1000 (Tecan) microplate reader, and the absorbance values at 540 nm were subtracted from 450 nm to calculate CXCL9 level using a standard curve derived from the known concentration of the recombinant proteins. Mouse CRG-2 (CXCL10) ELISA kit (Invitrogen, #EMCXCL10) was used according to manufacturer’s instructions. Optical densities at 450 nm were measured using a SpectraMax M5 microplate reader.