Kinetex evo c18 100a

The HPLC analyses of the phenolic extracts were conducted according to Selvaggini et al. [28 (link)], with a reversed-phase column using a HPLC-DAD, Varian ProStar-Diode Array Detector 330. The machinery was composed of a vacuum degasser, a quaternary pump, an autosampler, a thermostatic column compartment, and a DAD, using a 250 × 4.6 mm column 5 µm Kinetex EVO C18 100A (Phenomenex, Torrance, CA, USA). Eluent “A” was made with water and phosphoric acid 0.2% (Carlo Erba, Milano, Italy) and eluent “B” was methanol/acetonitrile (Carlo Erba) 50:50 (v/v). The elution gradient started from 4% eluent B and reached 100% B after 55 min for 15 min at a flow rate of 1.2 mL min⁻¹. Phenolic compounds were quantified at three wavelengths: 280, 310, and 360 nm using an authentic external standard. Secoiridoid derivatives, oleacein (3,4-DHPEA-EDA), and oleocanthal (p-HPEA-EDA) with 95% purity were provided by Prof. P. Magiatis (University of Athens, Greece). All other identified phenols were purchased from Sigma-Aldrich (St. Louis, MO, USA).