Extracts of M. edulis (50 mg ml-1, in methanol for 24 h) were analyzed by LC-ESIMS using an LTQ Orbitrap XL, linear ion trap/orbitrap hybrid mass spectrometer (Thermo Scientific, San Jose, CA, United States) with an electrospray ionization source (Ion Max, Thermo Scientific) coupled to an Acella 1250 uPLC system (Thermo Scientific). Samples were injected onto a Phenomenex Luna C18(2) column (150 × 3 mm i.d., 3 μm particle size) and eluted at 400 μL min-1 using a linear gradient as described previously (Green et al., 2017 (link)) and compounds eluted using MeOH (A), H2O (B), and formic Acid (C) with A = 0%, B = 90% at T = 0 min; A = 90%, B = 0% at T = 20 min and held for 10 min with C at 10% throughout the analyses. Column temperature was 30°C with flow rate = 0.5 ml min-1. Samples were scanned, using FTMS, from m/z 125–1250 in both positive and negative modes.
Acella 1250 uplc system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Acella 1250 uPLC system is an ultra-high-performance liquid chromatography (uPLC) instrument designed for analytical applications. The system features a high-pressure pump, a low-volume sample injection system, and a sensitive UV-Vis detector. It is capable of delivering precise and accurate results for a variety of analytical tasks.
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2 protocols using acella 1250 uplc system
1
Comprehensive LC-ESIMS Analysis of M. edulis
2
Compound Identification in Plant Extracts
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Accurate mass measurements of compounds detected in the extracts were obtained using an LTQ Orbitrap XL, linear ion trap/orbitrap hybrid mass spectrometer (Thermo Scientific, San Jose, California USA) with an electrospray ionisation source (Ion Max, Thermo Scientific) coupled to an "Acella 1250" UPLCsystem (Thermo Scientific). Samples were injected onto a Phenomenex Luna C18(2) column (150 × 3mm i.d., 3µm particle size) at 400µL min -1 and eluted using a linear gradient of 90:0: 10 (t=0min) to 0:90:10 (t=20-25min), returning to 90:0:10 (t=27-30min). Solvents were water, methanol and 1% formic acid in acetonitrile, respectively. The column was maintained at 30ºC. Samples were scanned, using FTMS, from m/z 250-2000 in both positive and negative modes. Samples were first matched with those reported from T. diversifolia and V. amygdalina in the Combined Chemical Dictionary(CCD, 2017), by using the m/z to calculate putative molecular formulae. Where compounds could not be matched to compounds known from these two species the search was extended first to the generic level, then to the Asteraceae and finally to other sources.
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