Arabidopsis leaves were fixed in glutaraldehyde (2.5%, v/v) for overnight. Post fixation was done for 1–2 h by soaking the samples in 1% (v/v) osmium tetroxide. Then, the samples were dehydrated in series acetone and subsequently embedded in Epon 812. Thin sections were cut using the ultramicrotome (Ultracut F-701704, Reichert-Jung, Reichert, Austria) and negatively stained with uranyl acetate (2%) on glow discharged carbon-coated copper grids. The ultrastructures of chloroplast were observed with the TEM H-9500 electron microscope (Itachi, Tokyo, Japan) at 75 kV. A quantitative analysis of the thylakoid membrane complexes was performed using the Quantity One software (Bio-Rad Comp. Hercules, CA, USA). Analysis for the area of chloroplast and starch granule was performed using ImageJ [66 (link)].
Ultracut f 701704
Manufactured by Reichert Technologies
Sourced in Austria
The Ultracut F-701704 is a precision instrument designed for microtome sectioning. It features a high-performance cutting mechanism and advanced ergonomic controls for accurate and efficient sample preparation.
Automatically generated - may contain errors
6 protocols using ultracut f 701704
1
Ultrastructural Analysis of Arabidopsis Chloroplasts
2
Thylakoid Ultrastructure Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thylakoid ultrastructure was analyzed according to Liu et al. (2009 (link)). Transverse section of the leaf samples were fixed with 3% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 6.9) overnight at 4°C, then fixed with 1% osmium tetroxide, dehydrated with acetone, and embedded in Epon 812. Thin sections were cut with an ultramicrotome (Ultracut F-701704, Reichert-Jung, Reichert, Austria) and were negatively stained with 2% uranyl acetate on glow-discharged carbon-coated copper grids. Electron microscopy was performed using a TEM H600 electron microscope (Hitachi, Midland, ON, Canada) operating at 100 kV.
3
Ultrastructural Analysis of Leaf Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) analysis of leaves was carried out following a previous method [74 (link)]. Leaf tissue was fixed with 3% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 6.9) at 4 °C overnight, after being washed with phosphate buffer three times. Samples were post-fixed with 2.5% osmium tetroxide, then dehydrated in a gradient solution of alcohol–acetone mixture and embedded in Epon Ultrathin cross sections were cut with an ultramicrotome (Ultracut F-701704, Reichert-Jung, Reichert, Austria), which was then stained with uranyl acetate and observed using a transmission electron microscope (TEM H-9500, Itachi, Tokyo, Japan) operating at 75 kV.
4
Ultrastructural Analysis of Wheat Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Structure of wheat roots and leaves after two-week exposure at 5 g nTiO2 L−1 was observed using a transmission electron microscope (TEM H-9500, Hitachi, Tokyo, Japan) operating at 75 kV. The root tips and leaves were prefixed with 0.1 M sodium cacodylate buffer (pH 6.9) containing 3% glutaraldehyde at 4 °C overnight, and then fixed in 1% osmium tetroxide for 1 h. The specimens were subsequently dehydrated with acetone and embedded in Epon 812. Thin sections were cut using an ultra-thin microtome (Ultracut F-701704, Reichert Jung, Reichert, Austria) and then were stained with 2% uranyl acetate on a copper-coated copper grid with glow discharge.
5
Transmission Electron Microscopy of Plant Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pieces of leaf tissue from control and infected plants were fixed immediately with 3% glutaraldehyde in 0.1 sodium cacodylate buffer (pH 6.9) at 4°C overnight, post-fixed with 1% osmium tetroxide, dehydrated in series acetone and embedded in Epon 812, as described previously (Liu et al., 2009 (link)). Thin sections cut with an ultramicrotome (Ultracut F-701704, Reichert-Jung, Austria) were stained with uranyl acetate and observed in a Transmission Electron Microscope (TEM H600, Hitachi) operating at 100 kV.
6
Ultrastructure Analysis of Wheat Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thylakoid ultrastructure was analyzed according to Liu et al.29 (link). Wheat leaf samples from drought-resistant and drought-susceptible wheat were fixed with 3% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 6.9) overnight at 4 °C, then fixed with 1% osmium tetroxide, dehydrated with acetone and embedded in Epon 812. Thin sections were cut with an ultramicrotome (Ultracut F-701704, Reichert-Jung, Reichert, Austria) were negatively stained with 2% uranyl acetate on glow discharged carbon-coated copper grids. Electron microscopy was performed using a TEM H600 electron microscope Hitachi, Midland, ON, Canada) operating at 100 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!