The human triple negative breast cancer cell line, HCC1937, and the mammary gland cell line, MCF-10A, were donated by Professor Ceshi Chen from the Kunming Institute of Zoology (Kunming, China), CAS (Chinese Academy of Sciences). was recovered from cryopreservation in liquid nitrogen (−196°C) and used at an early passage. 293 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HCC1937 cells were maintained in RPMI-1640 medium (HyClone; GE Healthcare Life Sciences, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; HyClone; GE Healthcare Life Sciences), 10 U/ml penicillin and 0.1 mg/ml streptomycin (HyClone; GE Healthcare Life Sciences). MCF-10A cells were maintained in Dulbecco's Modified Eagle's medium (DMEM)-F12 medium (HyClone; GE Healthcare Life Sciences) supplemented with 10% FBS, 10 U/ml penicillin and 0.1 mg/ml streptomycin. 293 cells were maintained in Dulbecco's Modified Eagle's medium (DMEM; HyClone; GE Healthcare Life Sciences) supplemented with 10% FBS, 10 U/ml penicillin and 0.1 mg/ml streptomycin. HCC1937, MCF-10A and 293 cells were then incubated at 37°C in 5% CO2.
Streptomycin
Manufactured by GE Healthcare
Sourced in United States, Germany, Austria, United Kingdom, France, Canada, Belgium, Italy, Slovakia, China, Sweden, Switzerland
Streptomycin is a type of antibiotic laboratory equipment used for the detection and identification of streptococcal bacteria. It serves as a diagnostic tool in microbiology laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by GE Healthcare (342 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (58 mentions)
Fetal bovine serum (fbs), by GE Healthcare (45 mentions)
L glutamine, by GE Healthcare (45 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (39 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (35 mentions)
24 flat bottom well culture plates, by Thermo Fisher Scientific (29 mentions)
Facscanto 2 flow cytometer, by BD (26 mentions)
Dulbecco modified eagle medium (dmem), by GE Healthcare (24 mentions)
Mouse inflammation cytometric bead assay cba, by BD (23 mentions)
Hyclone, by GE Healthcare (20 mentions)
Rpmi 1640, by GE Healthcare (19 mentions)
Rpmi 1640 medium, by GE Healthcare (16 mentions)
Penicillin, by Thermo Fisher Scientific (16 mentions)
Streptomycin, by Thermo Fisher Scientific (16 mentions)
Fetal calf serum (fcs), by GE Healthcare (15 mentions)
Fetal bovine serum (fbs), by Merck Group (15 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (14 mentions)
L glutamine, by Thermo Fisher Scientific (12 mentions)
Penicillin g, by GE Healthcare (11 mentions)
383 protocols using streptomycin
1
Cell Line Maintenance Protocol
2
Cultivation and Autophagy Induction in Cell Lines
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hTERT-RPE1 cells were obtained from the American Type Culture Collection (ATCC CRL-4000) and grown in Dulbecco's modified Eagle's medium (DMEM)/F-12, GlutaMAX medium (Gibco) supplemented with 10% (v/v) fetal bovine serum (Gibco), 200 μg/ml Hygromycin B, 50 U/ml penicillin, and 50 μg/ml streptomycin (GE Healthcare) at 37 °C and 5% CO2. U2OS, 293, and 293T cells were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ no. ACC 785, ACC 305, and ACC 635, respectively) and grown in DMEM, GlutaMAX medium (Gibco) supplemented with 10% (v/v) fetal bovine serum (Gibco), and 50 U/ml penicillin and 50 μg/ml streptomycin (GE Healthcare) at 37 °C and 5 % CO2. PCR-based Mycoplasma contamination tests were regularly performed using the VenorGeM Classic kit (Minerva Biolabs). Autophagy was induced by amino acid starvation using EBSS medium (Gibco).
3
Gastric Cancer Tissue and Cell Line Protocols
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GC tissue samples and paired normal tissue samples were obtained from 47 patients who underwent surgery between March 2017 and March 2018 at Chengyang People's Hospital (Qingdao, Shandong, China) with written informed consent. All samples were frozen in liquid nitrogen and stored at −80°C until use. The present study was approved by the Ethics Committee of Chengyang People's Hospital. GC tissues were fixed in 4% buffered paraformaldehyde for 24 h at 4°C, embedded in paraffin and then sectioned to 5 µm. These sections were stained with hematoxylin for 15 min and eosin for 5 min at 25°C. Samples were examined under a light microscope at a magnification of ×400. The human gastric epithelial mucosa cell line GES-1 and GC cell lines HGC-27, AGS, MKN45 and NCI-N87 were purchased from Nanjing Keygen Biotech Co., Ltd. HGC-27, AGS, MKN45 and NCI-N87 cells were maintained in RPMI-1640 with 10% foetal bovine serum (FBS), 100 U/ml penicillin and 100 µg/ml streptomycin (HyClone; GE Healthcare Life Sciences). GES-1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with FBS, 100 U/ml penicillin and 100 µg/ml streptomycin (HyClone; GE Healthcare Life Sciences). All cells were cultured in a humidified chamber with 5% CO2 at 37°C.
4
Evaluating TP53 Mutant CRC Cells
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Human HCT116 (WT TP53), HT-29 (mutant TP53), and DLD-1 (mutant TP53) CRC cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in RPMI-1640 (GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences), 100 units/mL penicillin, and 100 μg/mL streptomycin (GE Healthcare Life Sciences) at 37 °C in a humidified atmosphere with 5% CO2. The NCM460D cells were provided by Professor Eunok Im (Pusan National University, Busan, Korea). This cell line was incubated in DMEM (GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (GE Healthcare Life Sciences), 100 units/mL penicillin, and 100 μg/mL streptomycin (GE Healthcare Life Sciences) and cultured with 5% CO2 at 37 °C. Cell viability was measured using the MTT assay. Cells were seeded in 24-well culture plates and cultured for 24 h or 48 h before treatment with or without various reagents at the indicated concentrations. Cells were incubated in the dark with MTT (0.5 mg/mL) at 37 °C for 2 h. The formazan granules generated by the live cells were dissolved in DMSO, and the absorbance was measured at 540 nm using a multi-well plate reader (Thermo Fisher Scientific, Waltham, MA, USA).
5
Culturing Cell Lines for Cancer Research
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Saos-2 osteosarcoma cells (Cat. No. HTB-85) and Daoy medulloblastoma cells (Cat. No. HTB-186™) were purchased from the American Type Culture Collection (Manassas, VA, USA). SH-SY5Y neuroblastoma cells (Cat. No. 94030304) were purchased from The European Collection of Authenticated Cell Cultures (Salisbury, UK).
The Daoy and Saos-2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, and the antibiotics, penicillin (100 IU/mL) and streptomycin (100 μg/mL) (all from GE Healthcare Europe GmbH, Freiburg, Germany). The medium used for Daoy cells was supplemented with 1% non-essential amino acids (Biosera, Nuaille, France).
SH-SY5Y cells were cultured in a mixture of DMEM/F12 (1:1) supplemented with 20% FCS, 2 mM glutamine and the antibiotics, penicillin (100 IU/mL) and streptomycin (100 μg/mL) (all from GE Healthcare), and 1% nonessential amino acids (Biosera).
All cells were maintained under standard cell culture conditions at 37 °C in an atmosphere of 95% air, 5% CO2.
The Daoy and Saos-2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, and the antibiotics, penicillin (100 IU/mL) and streptomycin (100 μg/mL) (all from GE Healthcare Europe GmbH, Freiburg, Germany). The medium used for Daoy cells was supplemented with 1% non-essential amino acids (Biosera, Nuaille, France).
SH-SY5Y cells were cultured in a mixture of DMEM/F12 (1:1) supplemented with 20% FCS, 2 mM glutamine and the antibiotics, penicillin (100 IU/mL) and streptomycin (100 μg/mL) (all from GE Healthcare), and 1% nonessential amino acids (Biosera).
All cells were maintained under standard cell culture conditions at 37 °C in an atmosphere of 95% air, 5% CO2.
6
Herpes Simplex Virus Stock Production and Reactivation
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All viruses were derived from HSV-1 strain SC16 [43 (link)]. Baby hamster kidney (BHK) cells (American Type Culture Collection CCL-10) were used for virus stock production and plaque assays and were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal calf serum (FCS), 10% tryptose phosphate broth, 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml) (PAA laboratories). MRC5 cells (American Type Culture Collection CCL-171) were used for reactivation experiments and were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal calf serum (FCS), 2 mM L-glutamine, penicillin (100 U/ml), streptomycin (100 μg/ml) (PAA laboratories) and supplemented with non-essential amino acids (Gibco). All cells were incubated at 37°C and 5% CO2.
7
Culturing Immortal Cell Lines for Research
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Macaca mulatta kidney epithelial cell line (LLC-MK2) (ATCC: CCL-7 cell line) was maintained in minimal essential medium (MEM) containing 2 parts of Hank's MEM and 1 part of Earle's MEM (PAA Laboratories) supplemented with 3% heat-inactivated fetal bovine serum (PAA Laboratories), penicillin (100 U/ml), and streptomycin (100 μg/ml) (3% H&E). Murine L cells stably transfected with the N-Ceacam receptor (LR7) were maintained in Dulbecco-modified Eagle's medium (DMEM) (PAA Laboratories) supplemented with 3% heat-inactivated fetal bovine serum (PAA Laboratories), penicillin (100 U/ml), streptomycin (100 μg/ml), and G418 (50 μg/ml, Sigma-Aldrich) (3% DMEM). Human colon carcinoma cell line (HCT-8) (ATCC: CCL-244) was maintained in RPMI-1640 medium supplemented with 3% heat-inactivated fetal bovine serum (PAA Laboratories), penicillin (100 U/ml), and streptomycin (100 μg/ml) (i.e., 3% RPMI). Cells were cultured on T25 flasks (TPP) at 37 °C with 5% CO2 and 95% humidity. For cell maintenance and storage cell culture media were additionally supplemented with ciprofloxacin (5 μg/ml).
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Macaca mulatta kidney epithelial cell line (LLC-MK2) (ATCC: CCL-7 cell line) was maintained in minimal essential medium (MEM) containing 2 parts of Hank's MEM and 1 part of Earle's MEM (PAA Laboratories) supplemented with 3% heat-inactivated fetal bovine serum (PAA Laboratories), penicillin (100 U/ml), and streptomycin (100 μg/ml) (3% H&E). Murine L cells stably transfected with the N-Ceacam receptor (LR7) were maintained in Dulbecco-modified Eagle's medium (DMEM) (PAA Laboratories) supplemented with 3% heat-inactivated fetal bovine serum (PAA Laboratories), penicillin (100 U/ml), streptomycin (100 μg/ml), and G418 (50 μg/ml, Sigma-Aldrich) (3% DMEM). Human colon carcinoma cell line (HCT-8) (ATCC: CCL-244) was maintained in RPMI-1640 medium supplemented with 3% heat-inactivated fetal bovine serum (PAA Laboratories), penicillin (100 U/ml), and streptomycin (100 μg/ml) (i.e., 3% RPMI). Cells were cultured on T25 flasks (TPP) at 37 °C with 5% CO2 and 95% humidity. For cell maintenance and storage cell culture media were additionally supplemented with ciprofloxacin (5 μg/ml).
8
Isolation and Culture of Murine Synovial Fibroblasts and Cartilage
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SFs were isolated from the hind paws of WT and hTNFtg mice. The skin and surrounding tissue of the hind paw were removed, and the synovial tissue was exposed to enzymatic digestion as previously described [18 ]. Cells were then cultured in DMEM (Sigma-Aldrich, Steinheim, Germany) at 37 °C in a 5% CO2 atmosphere. The medium was supplemented with 10% fetal calf serum (FCS; Biochrom, Berlin, Germany), 100 U/ml penicillin, and 10 μg/ml streptomycin (PAA Laboratories, Pasching, Austria). Cells at passages 3–5 were used for all experiments.
Isolation of murine femoral hip cartilage was performed as previously described [13 (link)]. In short, WT mice were killed at an age of 4–5 weeks, hip joints were dissected, and cartilage caps were isolated aseptically. Cartilage tissue was then cultivated in high-glucose DMEM containing 10% FCS, 100 U/ml penicillin, and 10 μg/ml streptomycin (PAA Laboratories, Pasching, Austria), 10 mM HEPES, and 50 μg/500 ml ascorbic acid for 24 h at 37 °C in a 5% CO2 atmosphere.
Isolation of murine femoral hip cartilage was performed as previously described [13 (link)]. In short, WT mice were killed at an age of 4–5 weeks, hip joints were dissected, and cartilage caps were isolated aseptically. Cartilage tissue was then cultivated in high-glucose DMEM containing 10% FCS, 100 U/ml penicillin, and 10 μg/ml streptomycin (PAA Laboratories, Pasching, Austria), 10 mM HEPES, and 50 μg/500 ml ascorbic acid for 24 h at 37 °C in a 5% CO2 atmosphere.
9
Culturing Human Gingival Fibroblasts and SCC-25 Cells
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Human gingival fibroblasts (FIBs) were purchased from the German Cell Line Service (CLS, Eppelheim, Germany) and routinely cultured in Dulbecco’s modified Eagle’s medium (DMEM; with 1 g/L glucose; Biowhittaker®, Verviers, Belgium) supplemented with 10% fetal bovine serum (FBS; Gibco®, Auckland, New Zealand), 2 mM l-glutamine (Gibco®, Paisley, UK), 100 units/mL penicillin, and 100 μg/mL streptomycin (PAA Laboratories, Pasching, Austria). SCC-25 cells were acquired from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany, DSMZ no.: ACC 617). As described above, FIB cells were routinely cultured in serum-containing DMEM medium with low glucose and SCC-25 cells in serum-containing DMEM/F12 medium with high glucose, whereas for experimental purposes both the FIB cells and SCC-25 cells were cultured in an albumin-containing medium (1:1 ratio of DMEM-low glucose and DMEM-high glucose/Ham’s F12 supplemented with 4.4 g/L BSA from PAA Laboratories, Pasching, Austria, 2 mM l -glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin) to replace the whole protein content of the full culture medium.
Research using human material including the used cell lines was approved by the Ethic Commission of the Medical University of Innsbruck (ref. number: UN3678).
Research using human material including the used cell lines was approved by the Ethic Commission of the Medical University of Innsbruck (ref. number: UN3678).
10
Murine Skeletal Myoblast Differentiation
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The murine skeletal myoblast cell line PMI28 [18 ] was cultured in a growth medium composed of Ham’s F10 (PAA Laboratories GmbH, Pasching, Austria), supplemented with 20% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), 2 mM L-glutamine (PAA Laboratories), and Penicillin (100 I.U./ml) / Streptomycin (100 μg/ml, PAA Laboratories). 24 hours after seeding of the cells the growth medium was replaced by a differentiation medium containing DMEM medium with 2% horse serum (Gibco, Life Technologies GmbH, Darmstadt, Germany), 2 mM L-glutamine (PAA Laboratories), and Penicillin (100 I.U./ml) / Streptomycin (100 μg/ml) (PAA Laboratories). The differentiation medium of the treatment groups additionally contained 2 x 103 U/ml murine recombinant TNF-α (Roche Diagnostics, Rotkreuz, Switzerland) or 5 ng/ml murine recombinant IGF1 (Sigma-Aldrich). The control and treatment media were replenished twice a day to ensure cytokine and growth factor activity. Murine PMI28 cells were harvested 24 h after the induction of fusion by serum withdrawal for RNA analyses. Cells were propagated and differentiated at 37°C in 80% relative humidity and 5% CO2.
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