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Hel 92

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The HEL 92.1.7 is a cell line derived from human embryonic lung fibroblasts. It is a standardized and characterized cell line that can be used for various in vitro applications.

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Lab products found in correlation

Mv4 11, by American Type Culture Collection (2 mentions) Thp 1, by American Type Culture Collection (2 mentions) Hl 60, by American Type Culture Collection (2 mentions) Meg 01, by American Type Culture Collection (1 mentions) Rituximab, by Genentech (1 mentions) Coelenterazine, by Nanolight Technology (1 mentions) Digitonin, by Merck Group (1 mentions) Nk 92mi, by American Type Culture Collection (1 mentions) Blinatumomab, by Amgen (1 mentions) Cd3 magnetic microbeads, by Miltenyi Biotec (1 mentions) 293ft cell, by Thermo Fisher Scientific (1 mentions) Polybrene, by Merck Group (1 mentions) X vivo 15, by Lonza (1 mentions) Calcein am fluorescent dye, by BD (1 mentions) Glycine, by Thermo Fisher Scientific (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Pen strep neo solution, by Thermo Fisher Scientific (1 mentions) Phenol red free culture medium, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions)

12 protocols using hel 92

1

Culturing Diverse Cell Lines

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Bone marrow derived cell lines, MEG-01 (ATCC, CRL-2021) and HEL92.1.7 (ATCC, TIB-180) were grown in RPMI 1640 media and kept at 37 °C in 5% carbon dioxide (CO2) incubator. Chinese hamster ovary (CHO)-K1 cells (ATCC, CCL-61) and human embryonic kidney (HEK293) cells (ATCC, CRL-1573) were cultured in DMEM media and kept in a 5% CO2 incubator at 37 °C. ADRA2B-HEK293 cells were stably transfected with pcDNA3.1(+)-ADRA2B-N-DYK and G418-resistant cell pool expanded after being selected with G418 (1.5 mg/ml). Unless otherwise stated, all culture media were supplemented with fetal bovine serum (FBS; 10%), penicillin (100 U/mL) and streptomycin (100 μg/mL). β2-HEK293 were cultured as previously described (Watson et al., 2016 (link)). Before use, all cell lines used were tested to confirm negative status for mycoplasma (CC cell culture facility).
Nemet I., Saha P.P., Gupta N., Zhu W., Romano K.A., Skye S.M., Cajka T., Mohan M.L., Li L., Wu Y., Funabashi M., Ramer-Tait A.E., Prasad S.V., Fiehn O., Rey F.E., Tang W.H., Fischbach M.A., DiDonato J.A, & Hazen S.L. (2020). A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic Receptors. Cell, 180(5), 862-877.e22.
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2

Cell Lines Used for Experiments

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OCI-AML5, OCI-AML2, Mono-Mac-1, MOLM13, SKM-1, and SET-2 cells cells were obtained from the DSMZ. MV4-11, HEL92.1.7, THP1, and HS5 cells were obtained from the ATCC (Manassas, VA). HEK-293T cells were obtained from the Characterized Cell Line Core (CCLC) at M.D. Anderson Cancer Center, Houston TX. Experiments with cell lines were performed within 6 months after thawing or obtaining from ATCC or DSMZ. Cell lines were authenticated in the CCLC at M.D. Anderson Cancer Center. Logarithmically growing, mycoplasma-negative cells were utilized for all experiments.
Fiskus W., Mill C.P., Nabet B., Perera D., Birdwell C., Manshouri T., Lara B., Kadia T.M., DiNardo C., Takahashi K., Daver N., Bose P., Masarova L., Pemmaraju N., Kornblau S., Borthakur G., Montalban-Bravo G., Manero G.G., Sharma S., Stubbs M., Su X., Green M.R., Coarfa C., Verstovsek S., Khoury J.D., Vakoc C.R, & Bhalla K.N. (2021). Superior efficacy of co-targeting GFI1/KDM1A and BRD4 against AML and post-MPN secondary AML cells. Blood Cancer Journal, 11(5), 98.
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3

Cultivation and Characterization of Diverse Cell Lines

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K562 (Chronic Myelogenous Leukemia), Raji (Burkitt’s lymphoma), HEL 92.1.7 (Erythroleukemia) and NK92MI cell lines were obtained from ATCC and maintained as per the instructions provided. Nalm6 (Acute Lymphoblastic Leukemia), BC-1 (Primary effusion lymphoma), and KG-1 (Acute myelogenous leukemia) cell lines were kindly provided by Drs. Markus Muschen, Jae Jung, and Alan Epstein, respectively. 293FT cells were obtained from Invitrogen (ThermoFisher Scientific) and cultured as recommended. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient method from platelet depleted donor cells obtained from a Blood Bank. PBMCs were subsequently used to isolate T cells using CD3 magnetic microbeads (Miltenyi Biotech) following the manufacturer’s instructions. T cells were cultured in XVIVO-15 (Lonza) medium supplemented with 100 IU/ml recombinant human IL2 and 30 ng/ml soluble antibodies to human CD3 and CD28. All the cells were cultured at 37 °C, in a 5% CO2 humidified incubator. Blinatumomab was obtained from Amgen, Rituximab was obtained from Genentech. Digitonin, and Polybrene were from Sigma. Coelenterazine was purchased from Nanolight technology. Calcein AM fluorescent dye was obtained from BD biosciences.
Matta H., Gopalakrishnan R., Choi S., Prakash R., Natarajan V., Prins R., Gong S., Chitnis S.D., Kahn M., Han X., Chaudhary V., Soni A., Sernas J., Khan P., Wang D, & Chaudhary P.M. (2018). Development and characterization of a novel luciferase based cytotoxicity assay. Scientific Reports, 8, 199.
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4

Culturing K562 and HEL 92.1.7 Leukemia Cells

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The human chronic myelogenous leukemia cell line K562 (Cat No. CCL-243; ATCC) and human erythroleukemia cell line HEL 92.1.7 (Cat No. TIB-180; ATCC) were obtained from the American Type Culture Collection. Both the cells were grown in RPMI 1640 medium (Gibco) supplemented with 10% FBS (Sigma-Aldrich), gentamicin (1,000x, Cat No. 15-710-072; Thermo Fisher Scientific), Pen-Strep-Neo solution (100x, Cat No. 15640-055; Thermo Fisher Scientific), and 2 mM L-glutamine at 37°C in a humidified 5% CO2 atmosphere. 5-Aminolevulinic acid hydrochloride (ALA), purchased from Alfa Aesar (Cat No. A16942ME), was dissolved in distilled water to yield a stock concentration of 1.0 M, and stored at −20°C. Glycine, purchased from Thermo Fisher Scientific (Cat No. BP381-500), was dissolved in phenol red–free culture medium purchased from Gibco (Cat No. 11835055) to give a stock concentration of 1 M.
Adapa S.R., Hunter G.A., Amin N.E., Marinescu C., Borsky A., Sagatys E.M., Sebti S.M., Reuther G.W., Ferreira G.C, & Jiang R.H. (2024). Porphyrin overdrive rewires cancer cell metabolism. Life Science Alliance, 7(7), e202302547.
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5

Endothelial Cell Response to Inflammatory Stimuli

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We obtained the following: human umbilical vein endothelial cells (HUVECs), human coronary artery endothelial cells (HCAECs), normal human cardiac fibroblasts-ventricular (NHCF-V) cells, human erythroleukemia (HEL 92.1.7), and human neuroblastoma cells (SK-N-FI) from the American Type Culture Collection (ATCC). The human embryonic stem cell-derived cardiomyocytes (hESC-CMs) were differentiated from the Wicell H7 line. The hiPSC-derived neurons were provided by Dr. Thomas Sudhof (Department of Molecular and Cellular Physiology, Stanford University). TNFα and genistein treatment of hiPSC-ECs were performed on hiPSCs from healthy control line 1 or CRISPRi knockdown of CNR1. The hiPSCs were differentiated into hiPSC-ECs and treated with vehicle, 10 ng/ml TNFα, or 10 μM genistein. Gene expression was quantified using quantitative real-time PCR (qRT-PCR).
Wei T.T., Chandy M., Nishiga M., Zhang A., Kumar K.K., Thomas D., Manhas A., Rhee S., Justesen J.M., Chen I.Y., Wo H.T., Khanamiri S., Yang J.Y., Seidl F.J., Burns N.Z., Liu C., Sayed N., Shie J.J., Yeh C.F., Yang K.C., Lau E., Lynch K.L., Rivas M., Kobilka B.K, & Wu J.C. (2022). Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. Cell, 185(10), 1676-1693.e23.
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6

Cell Line Authentication and Culture

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All cell lines were grown under 5% CO2 at 37°C. Cell lines were obtained as follows: HL-60, U937, HEL 92.1.7 and THP.1 from American Type Culture Collection (Manassas, VA); ML-2, Molm13, and SET2 from Raoul Tibes (New York University); ML-1, K562, Jurkat and Z181 from Michael Kastan (Duke University), Robert Abraham (Pfizer), Paul Leibson (Mayo Clinic Rochester) and Joya Chandra (M.D. Anderson Cancer Center), respectively. All lines were authenticated by short tandem repeat profiling in the Mayo Clinic Cytogenetics Core, verified to be mycoplasma free, maintained at <1 × 106 cells/ml in RPMI 1640 containing 20% (SET2) or 10% (other lines) heat-inactivated fetal bovine serum (FBS), and passaged <3 months before use.
Ding H., Vincelette N.D., McGehee C.D., Kohorst M.A., Koh B.D., Venkatachalam A., Wei Meng X., Schneider P.A., Flatten K.S., Peterson K.L., Correia C., Lee S.H., Patnaik M., Webster J.A., Ghiaur G., Douglas Smith B., Karp J.E., Pratz K.W., Li H., Karnitz L.M, & Kaufmann S.H. (2021). CDK2-mediated upregulation of TNFα as a mechanism of selective cytotoxicity in acute leukemia. Cancer research, 81(10), 2666-2678.
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Cultivation of AML Cell Lines

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The AML cell lines HEL 92.1.7, HL-60, MV4-11, and THP-1 were purchased from the American Type Culture Collection and grown in IMDM or RPMI medium supplemented with 10% FBS and 1% penicillin/streptomycin. The MOLM-14 cell line was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ) and grown in RPMI supplemented with 10% FBS and 1% penicillin/streptomycin. The SET-2 and UKE-1 cell lines were gifts from R. Levine (Memorial Sloan-Kettering Cancer Center, New York, NY). SET-2 cells were grown in RPMI medium supplemented with 10% FBS and 1% penicillin/streptomycin. UKE-1 cells were grown in IMDM supplemented with 10% FBS, 10% Horse Serum, 1 µM hydrocortisone, and 1% penicillin/streptomycin.
Stanley R.F., Piszczatowski R.T., Bartholdy B., Mitchell K., McKimpson W.M., Narayanagari S., Walter D., Todorova T.I., Hirsch C., Makishima H., Will B., McMahon C., Gritsman K., Maciejewski J.P., Kitsis R.N, & Steidl U. (2017). A myeloid tumor suppressor role for NOL3. The Journal of Experimental Medicine, 214(3), 753-771.
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8

Cell Culture Conditions for K562, HEL92.1.7, and TF-I

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K562, HEL92.1.7, and TF-I cell lines were purchased from American Type Culture Collection (ATCC). Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone), 2 mM l-glutamine (Sigma-Aldrich) 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma-Aldrich) at 37 °C in a humidified atmosphere of 5% CO2 in the air. Additionally, TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
Grzywa T.M., Sosnowska A., Rydzynska Z., Lazniewski M., Plewczynski D., Klicka K., Malecka-Gieldowska M., Rodziewicz-Lurzynska A., Ciepiela O., Justyniarska M., Pomper P., Grzybowski M.M., Blaszczyk R., Wegrzynowicz M., Tomaszewska A., Basak G., Golab J, & Nowis D. (2021). Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells. Communications Biology, 4, 1384.
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Cell Line Cryopreservation Protocol

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CCRF-CEM (human T lymphoblast cell line) and K562 (human myelogenous leukemia cell line) were obtained from the Japanese Collection of Research Bioresearch Cell Bank. HEL92.1.7 (human erythroblast cell line) was obtained from the American Type Culture Collection. The cells were stored under liquid nitrogen before use.
Yamamoto R., Yoden E., Tanaka N., Kinoshita M., Imakiire A., Hirato T, & Minami K. (2021). Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II. Molecular Genetics and Metabolism Reports, 27, 100758.
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Diverse Cell Lines for Biological Investigations

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Human embryonal kidney (HEK293) cells were purchased from the German Collection of Microorganism and Cell Cultures (DSMZ, Braunschweig, Germany). Cancer cell lines were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA): SK-MEL-3 (malignant melanoma; HTB-69), Capan-1 (pancreatic adenocarcinoma; HTB-79), HT-29 (colorectal adenocarcinoma; HTB-38), SK-ES-1 (Ewing´s sarcoma; HTB-86), HEL92.1.7 (erythroleukemia; TIB-180), Jurkat (acute T-cell leukemia; TIB-152), U-937 (histiocytic lymphoma; CRL-1593.2), LNCaP (prostate cancer; CRL-1740) and NCI-H460 (large cell lung cancer; HTB-177), and MG-63 (osteosarcoma; CRL-1427) cells. HaCaT human skin keratinocyte cells (cryovial, 300493) were from Cell Line Service (CLS, Eppelheim, Germany).
Huber S., Huettner J.P., Hacker K., Bernhardt G., König J, & Buschauer A. (2015). Esters of Bendamustine Are by Far More Potent Cytotoxic Agents than the Parent Compound against Human Sarcoma and Carcinoma Cells. PLoS ONE, 10(7), e0133743.
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