The human normal liver cell line (LO2), human embryonic kidney 293 T cell line, and HepG2 cell line were purchased from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM), with 10% FBS, 100 units/mL penicillin, 100 μg/mL streptomycin. Fetal bovine serum (FBS), DMEM, penicillin, and streptomycin were purchased from Gibco BRL (Gaithersburg, MD, USA).
Human embryonic kidney 293 t cell line
Manufactured by American Type Culture Collection
Sourced in United States
The Human embryonic kidney 293 T cell line is a widely used immortalized cell line derived from human embryonic kidney cells. It is a common tool used in cell biology and biotechnology research. The 293 T cell line retains the key properties of kidney cells and is known for its high transfection efficiency, making it a valuable resource for various experimental applications.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hepg2 cell line, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Sk n sh, by American Type Culture Collection (1 mentions)
Imr 32, by American Type Culture Collection (1 mentions)
Sk n be 2, by American Type Culture Collection (1 mentions)
Tpck trypsin, by Merck Group (1 mentions)
Lenti x 293t cell line, by Takara Bio (1 mentions)
Bovine albumin, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)
4 protocols using human embryonic kidney 293 t cell line
1
Cell Culture Conditions for Liver and Kidney Cells
2
Culturing Human Cancer Cell Lines
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Human CC cell lines and a HCC cell line, SH-J1, were kindly provided by Dr. Dae-Ghon Kim (Chonbuk National University Medical School, Korea) and grown in DMEM (WelGENE). Other HCC cell lines were generously provided by Dr. Kwan Yong Choi (POSTECH, Korea) and grown in RPMI1640 (WelGENE). Human embryonic kidney 293T cell line was purchased from American Type Culture Collection and maintained in recommended medium. All medium was supplemented with 10% FBS (Thermo Scientific) and antibiotic-antimycotic (Invitrogen).
3
Cell Culture of Neuroblastoma and Kidney Lines
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The human NB cell lines IMR-32, NB1, SK-N-SH, and SK-N-BE2 and the human embryonic kidney293T cell line were purchased from the American Type Culture Collection (United States). All cell lines were maintained in high glucose Dulbecco’s modified Eagle medium (Hyclone, United States) supplemented with 10% fetal bovine serum (Gibco Corporation, United States) and 1% penicillin–streptomycin (Solarbio Science & Technology, China). All cells were cultured in a humidified atmosphere containing 5% carbon dioxide at 37°C.
4
Cultivation of MDCK and HEK293T Cell Lines
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Madin-Darby Canine Kidney (MDCK-ATL) Cells, FR-926, and Madin-Darby Canine Kidney (MDCK) Cells, London Line, FR-58, were obtained through the Influenza Reagent Resource, Influenza Division, WHO Collaborating Center for Surveillance, Epidemiology and Control of Influenza, Centers for Disease Control and Prevention, Atlanta, GA. The human embryonic kidney 293T cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA), and the Lenti-X 293T cell line was obtained from Clontech (Mountain View, CA). Cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM; Lonza, Allendale, NJ) containing 10% fetal bovine serum (FBS; Thermo Scientific, Waltham, MA), 100 IU/ml penicillin, and 100 µg/ml streptomycin. All cells were incubated at 37°C and 5% CO2. Influenza virus-infected cells were grown in DMEM containing 0.5% bovine albumin and TPCK trypsin (Sigma-Aldrich, St. Louis, MO).
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