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Minute total protein extraction kit for adipose tissues

Manufactured by Invent Biotechnologies
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Sourced in United Kingdom

The Minute Total Protein Extraction Kit for Adipose Tissues is a laboratory product designed for the rapid and efficient extraction of total protein from adipose tissue samples. The kit provides a streamlined protocol to isolate protein samples suitable for further analysis, such as Western blotting or enzymatic assays.

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Lab products found in correlation

Anti tubulin, by Cell Signaling Technology (4 mentions) Ripa buffer, by Cell Signaling Technology (4 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Anti sirt1, by Cell Signaling Technology (2 mentions) Anti translin, by Thermo Fisher Scientific (2 mentions) Anti trax, by Thermo Fisher Scientific (2 mentions) Hsc70, by Thermo Fisher Scientific (2 mentions) Protease and phosphatase inhibitor, by Merck Group (2 mentions) Ecl system, by Thermo Fisher Scientific (2 mentions) Anti actin, by Merck Group (2 mentions) Of protease and phosphatase inhibitors, by Merck Group (2 mentions) Anti hsc70, by Merck Group (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions)

4 protocols using minute total protein extraction kit for adipose tissues

1

Liver Protein Extraction and Immunoblotting

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Liver samples were rinsed with PBS and homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN). Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane and immunoblotted with antibodies: Anti-SIRT1, anti-tubulin (#9475T and #2148, Cell Signaling Technology), anti-translin and anti-trax (generated in our laboratory, [13 (link)]) and HSC70 (#PA529221, Thermo Fisher Scientific). Blots were developed with the ECL system. Band intensities were quantified from digital images, by densitometry, using ImageJ.
Shah A.P., Johnson M.D., Fu X., Boersma G.J., Shah M., Wolfgang M.J., Tamashiro K.L, & Baraban J.M. (2019). Deletion of translin (Tsn) induces robust adiposity and hepatic steatosis without impairing glucose tolerance. International journal of obesity (2005), 44(1), 254-266.
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2

Liver Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver samples were rinsed with PBS and homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN). Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA). Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane and immunoblotted with antibodies: Anti-SIRT1, anti-tubulin (#9475T and #2148, Cell Signaling Technology), anti-translin and anti-trax (generated in our laboratory, [13 (link)]) and HSC70 (#PA529221, Thermo Fisher Scientific). Blots were developed with the ECL system. Band intensities were quantified from digital images, by densitometry, using ImageJ.
Shah A.P., Johnson M.D., Fu X., Boersma G.J., Shah M., Wolfgang M.J., Tamashiro K.L, & Baraban J.M. (2019). Deletion of translin (Tsn) induces robust adiposity and hepatic steatosis without impairing glucose tolerance. International journal of obesity (2005), 44(1), 254-266.
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3

Protein Extraction and Analysis from Tissues

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Forebrain and liver tissue samples were homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing a cocktail of protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN) following the manufacturer's instructions. Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane (Bio-Rad) and immunoblotted with antibodies: anti-HSC70 (MilliporeSigma, Burlington, MA), anti-actin (MilliporeSigma) and anti-tubulin (Cell Signaling Technology), anti-TN and anti-TX (generated in our lab). Blots were developed with the ECL system (Thermo Fisher Scientific). Band intensities were quantified from digital images by densitometry using ImageJ.
Fu X., Shah A.P., Li Z., Li M., Tamashiro K.L, & Baraban J.M. (2020). Genetic inactivation of the translin/trax microRNA-degrading enzyme phenocopies the robust adiposity induced by Translin (Tsn) deletion. Molecular Metabolism, 40, 101013.
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4

Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Forebrain and liver tissue samples were homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing a cocktail of protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN) following the manufacturer's instructions. Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane (Bio-Rad) and immunoblotted with antibodies: anti-HSC70 (MilliporeSigma, Burlington, MA), anti-actin (MilliporeSigma) and anti-tubulin (Cell Signaling Technology), anti-TN and anti-TX (generated in our lab). Blots were developed with the ECL system (Thermo Fisher Scientific). Band intensities were quantified from digital images by densitometry using ImageJ.
Fu X., Shah A., Li Z., Li M., Tamashiro K.L., & Baraban J.M. (2020). Genetic inactivation of the translin/trax microRNA-degrading enzyme phenocopies the robust adiposity induced by Translin (Tsn) deletion.
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