1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was acquired from Sigma-Aldrich (St Louis, MO, product #P2663). 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was acquired from Sigma-Aldrich (St Louis, MO, product #P0763). Lactated Ringer’s solution (LRS) was acquired from Patterson Veterinary (Greeley, CO, catalog #078696319). Dulbecco’s phosphate-buffered saline (PBS) without calcium chloride or magnesium chloride was from Sigma-Aldrich (St Louis, MO, catalog #D8537). TrophAmine 10% amino acid solution was acquired from Medline Industries (Northfield, IL, catalog #BMGS9341H). Ethanol was acquired from Sigma-Aldrich (St Louis, MO, catalog #E7023–500ml). Mouse albumin was acquired from Innovative Research (Novi, MI, catalog #IRMSA) and tested free of common murine pathogens (IDEXX, IMPACT 1 PCR Panel). Hexagonal boron nitride (HBN) was acquired from Sky Spring Nanomaterials, Inc (Houston, TX). Tetramethylrhodamine anionic lysine-fixable dextran (rhodamine dextran, 3000 MW, catalog #D3308) and Alexa Fluor 680 anionic dextran (3000 MW, catalog #D34681) were acquired from ThermoFisher (Waltham, MA).
1 2 dimyristoyl sn glycero 3 phosphocholine
Manufactured by Merck Group
Sourced in United States
1,2-dimyristoyl-sn-glycero-3-phosphocholine is a phospholipid compound commonly used in laboratory settings. It is a synthetic lipid molecule that can be used for various applications in research and development.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Merck Group (3 mentions)
1 2 distearoyl sn glycero 3 phosphocholine, by Merck Group (3 mentions)
Milli q system, by Merck Group (2 mentions)
1 2 dimyristoyl sn glycero 3 phospho 1 rac glycerol, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Dulbecco s phosphate buffered saline, by Merck Group (1 mentions)
Mouse albumin, by Innovative Research (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Merck Group (1 mentions)
Emparta acs, by Merck Group (1 mentions)
Lichrosolv water, by Merck Group (1 mentions)
Trichloromethane, by Merck Group (1 mentions)
Lincomycin, by Merck Group (1 mentions)
Oxytetracycline, by Merck Group (1 mentions)
Cannabidiol, by Merck Group (1 mentions)
Chloramphenicol, by Merck Group (1 mentions)
Tetracycline, by Merck Group (1 mentions)
Doxycycline, by Merck Group (1 mentions)
Baicalin, by Merck Group (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Berberine, by Merck Group (1 mentions)
10 protocols using 1 2 dimyristoyl sn glycero 3 phosphocholine
1
Lipid Nanoparticle Formulation and Characterization
2
Fabrication of Lipid Bilayer Substrates
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1,2-dipalmitoyl-sn-glycero-3-phosphocoline semisynthetic (≥99%, DPPC), 1,2-distearoyl-sn-glycero-3-phosphocholine (≥99%, DSPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (≥99%, DMPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lyophilized powder were acquired from Sigma-Aldrich Company (St. Louise, MO, USA) and used without further purification. Chloroform (99.0–99.4%), hydrogen peroxide (30–32%), sulfuric acid (95–97%, Emparta®ACS) and water LiChrosolv® for chromatography were obtained from Merck KGaA (Darmstadt, Germany).
Immersion oil for microscopy (UN 3082-9, PG III) was purchased from Merck Millipore (Billerica, MA, USA). Uncoated N-BK7 right angle prism (15 mm) was acquired from Edmund Optics (Barrington, NY, USA). Gold targets of 99.99% purity (Ø 57 mm × 0.2 mm) for argon sputter deposition was obtained from Ted Pella products (Redding, CA, USA).
A p-type <100> orientation silicon wafer was acquired from Siegert Wafer GmbH (Aachen, Germany). Substrate characteristics are: resistivity 10–20 Ω cm, thickness 525 ± 20 µm, diameter 100 ± 0.3 mm, prime grade, CZ growth (Czochralski process), boron doping (P/B type), Single Side Polishing (SSP) with 2 Semi-standard flats, Total Thickness Variation (TTV) <5 µm, bow <30 µm, warp <30 µm, particles < 10 of 0.3 µm.
Immersion oil for microscopy (UN 3082-9, PG III) was purchased from Merck Millipore (Billerica, MA, USA). Uncoated N-BK7 right angle prism (15 mm) was acquired from Edmund Optics (Barrington, NY, USA). Gold targets of 99.99% purity (Ø 57 mm × 0.2 mm) for argon sputter deposition was obtained from Ted Pella products (Redding, CA, USA).
A p-type <100> orientation silicon wafer was acquired from Siegert Wafer GmbH (Aachen, Germany). Substrate characteristics are: resistivity 10–20 Ω cm, thickness 525 ± 20 µm, diameter 100 ± 0.3 mm, prime grade, CZ growth (Czochralski process), boron doping (P/B type), Single Side Polishing (SSP) with 2 Semi-standard flats, Total Thickness Variation (TTV) <5 µm, bow <30 µm, warp <30 µm, particles < 10 of 0.3 µm.
3
Lipid-based Nanoparticle Characterization Protocol
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Tetracycline (TET), berberine (BB), kanamycin, ampicillin, doxycycline, oxyTetracycline, gentamicin, chloramphenicol, lincomycin, baicalin, capsicine, betaine, cannabidiol, glycine, gluutamine, tyrosine, ascorbic, Dulbecco's PBS (DPBS), thioflavin T (ThT), acetonitrile, trichloromethane, 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and cholesterol were purchased from Sigma-Aldrich (St. Louis, MO). All chemical reagents were analytical grade and were used as received without further purification. The ultrapure water (UP) used throughout all experiments was purified by a Milli-Q system (Millipore, Bedford, MA, USA). The raw liquid milk was purchased from the local pasture. 4% (w/v) paraformaldehyde, Hoechst 33342 and DiD dye were purchased from Beyotime Biotechnology (Shanghai, China).
High-performance liquid chromatography purified oligonucleotides (ODNs) were purchased from Shanghai Sangon Biological Engineering Technology & Services (Shanghai, China), and used without additional purification (SeeSupplementary Table S1 for details of DNA sequences used). Each sequence was diluted in UP into 100 μmol l−1. For better formation of secondary conformation, sequences were heated to 95 °C for 5 min and colded to room temperature overnight, and was stored at −20 °C before use.
High-performance liquid chromatography purified oligonucleotides (ODNs) were purchased from Shanghai Sangon Biological Engineering Technology & Services (Shanghai, China), and used without additional purification (See
4
Characterization of Cholesterol Analogs
5
Lipid Oxidation and Ferroptosis Assay
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Lipofectamine RNAiMAX reagent and Opti-MEM medium were obtained from Invitrogen (Carlsbad, CA, U.S.A.). RSL3 was purchased from Selleckchem (Houston, TX, U.S.A.). The mouse monoclonal antibody against β-actin, the rabbit polyclonal antibody against ACSL6 (SAB4500971), NaAsO2, heptadecanoyl CoA (17:0-CoA), and 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (14:0/14:0 phosphatidylcholine (PC)), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (14:0/14:0 PE), and 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (14:0/14:0 phosphatidylglycerol), were obtained from Sigma–Aldrich (St. Louis, MO, U.S.A.). The rabbit monoclonal antibody against ACSL4 (FACL4: ab155282) and the rabbit polyclonal antibody against ACSL5 (ab104892) were acquired from Abcam (Cambridge, MA, U.S.A.). Ferrostatin-1 was obtained from Cayman Chemicals (Ann Arbor, MI, U.S.A.). Necrostatin-1 was acquired from ChemScene LLC (Monmouth Junction, NJ, U.S.A.). CdCl2 was purchased from Wako Chemicals (Osaka, Japan). The rabbit polyclonal antibody against ACSL1 (H-65), the mouse monoclonal antibody against ACSL3 (H-9), and DPQ were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.). Z-VAD-FMK (z-VAD) was obtained from the Peptide Institute, Inc. (Osaka, Japan).
6
Lipid Membrane Composition Analysis
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Lipids, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), 1,2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1,2dimyristoyl-sn-glycero-3-phosphocholine (DMPC), were purchased from Sigma (St. Louis, MO, USA) and used without further purification.
7
Liposome Preparation with Phospholipids
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1,2-dimyristoyl-sn-glycero-3-phos-phocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3phosphocholine (DPPC) and 1,2-dis-tearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, bovine serum albumin (BSA) (MW ~ 66 kDa, Water Solubility 40 mg/mL), tablets of phosphate-buffered saline (PBS, pH 7.4) and ethanol ≥99.8% were purchased from Sigma-Aldrich. 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (DOPC) was purchased from Tokyo chemical industries. The chemical structures can be seen in Figure 1. (hydrocarbon tail length n=18).
8
Lipid Membrane Composition Analysis
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The phospholipids 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phospho-1′-rac-glycerol (DMPG), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and the cationic lipids 1,2-dimyristoyl-3-trimethylammonium-propane (DMTAP) and 1,2-stearoyl-3-trimethylammonium-propane (DSTAP) were purchased from Sigma-Aldrich (Merck KGaA, Germany). The fluorinated amphiphile 1H,1H-perfluorooctadecan-1-ol (PFOL) was purchased from Proactive Molecular Research (USA) and the perfluorooctadecanoic acid (PFOA) from Santa Cruz Biotechnology, Inc. (USA). High purity (99.5-99.9%) NaCl was purchased from Sigma-Aldrich. Hexane, methanol, and chloroform were of the highest commercial purity available and were purchased from Sigma-Aldrich (Merck KGaA, Germany).
9
Lipid Bilayer Formation and Characterization
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2.1. Materials: Lipid layers were prepared with 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), 1,2dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) purchased from Sigma-Aldrich and used as received without further purification. Hexane, LC-MS grade (Sigma-Aldrich), Isopropanol (Sigma-Aldrich) and Methanol, HPLC grade (Sigma-Aldrich), were used as solvent in the experiments. Hi-grade freshly cleaved mica substrates (Ted Pella,Inc) were used as support.
10
Bilirubin Antioxidant Assay Protocol
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All chemicals were of analytical grade: BR (>98% purity), CuCl 2 , D-penicillamine, catalase (CAT; C1345), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), buffer components (Sigma-Aldrich, St.
Louis, MO, USA), CuCl (Merck, Kenilworth, NJ, USA), FeCl 3 (Analytika Ltd., Prague, Czech Republic).
All experiments were performed using bidistilled deionized ultrapure (18 MΩ) water. Stock solutions of BR (1 mM) in 5 mM NaOH, PA, and CuCl (prepared in 1 M HCl; an aliquot of 1 M NaOH was used to neutralize the acid), as well as phosphate buffer (50 mM KH 2 PO 4 ; pH 7.4), and phosphate buffer saline (PBS: 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , 137 mM NaCl, 2.7 mM KCl; pH 7.4) were prepared each day, and kept light-protected on ice if needed. Incubation and measurements were conducted in the dark at 293 K, except for experiments with erythrocytes (310 K).
Louis, MO, USA), CuCl (Merck, Kenilworth, NJ, USA), FeCl 3 (Analytika Ltd., Prague, Czech Republic).
All experiments were performed using bidistilled deionized ultrapure (18 MΩ) water. Stock solutions of BR (1 mM) in 5 mM NaOH, PA, and CuCl (prepared in 1 M HCl; an aliquot of 1 M NaOH was used to neutralize the acid), as well as phosphate buffer (50 mM KH 2 PO 4 ; pH 7.4), and phosphate buffer saline (PBS: 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , 137 mM NaCl, 2.7 mM KCl; pH 7.4) were prepared each day, and kept light-protected on ice if needed. Incubation and measurements were conducted in the dark at 293 K, except for experiments with erythrocytes (310 K).
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