Hmw adiponectin

Manufactured by Merck Group

Sourced in United States

HMW adiponectin is a lab equipment product manufactured by Merck Group. It is a protein that exists in various oligomeric forms in the bloodstream. The high molecular weight (HMW) form of adiponectin is the most biologically active and is involved in various metabolic processes.

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Lab products found in correlation

Adiponectin, by Merck Group (2 mentions) Leptin, by Merck Group (2 mentions) Resistin, by Merck Group (1 mentions) Hs crp, by Calbiotech (1 mentions) Tnf α, by Merck Group (1 mentions) Sob r, by R&D Systems (1 mentions) Milliplex map mag human cytokine kit, by Merck Group (1 mentions) Immulite 1000 immunoassay system, by Siemens (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Fabp4, by BioVendor (1 mentions)

Spelling variants of this product

Adiponectin (84 citations)

5 protocols using hmw adiponectin

Adipokine Measurements in SWAN Cohort

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Assays were run on stored serum samples collected from the SWAN follow-up visit 6, corresponding to the MPS follow-up (2002–2003). At collection, a 12-hour fasting blood draw was performed within days 2–5 of the menstrual cycle if a woman was still menstruating. For women not regularly menstruating or if a blood sample was not obtainable in the day 2–5 window, a random fasting blood draw was obtained. For women who were postmenopausal, a blood sample was drawn at their clinic visit, scheduled to be on the anniversary of their previous visit. leptin, sOB-R, adiponectin and HMW adiponectin were determined at the University of Michigan in duplicate, using commercially available colorimetric enzyme immunoassay kits according to the manufacturer’s instructions (adiponectin, HMW adiponectin, resistin, and leptin, Millipore, St. Charles, MO and soluble leptin receptor and MCP-1, R& D systems, Minneapolis, MN). The mean coefficient of variation percent for duplicate samples for each subject and lower limit of detection, respectively, were: adiponectin: 4%, 0.78 ng/mL; HMW adiponectin: 8.1%, 0.5 ng/ml; resistin: 5%, 0.16 ng/mL; leptin: 4%, 0.5 ng/mL; MCP-1: 1.7%, 31.2 pg/mL, and soluble leptin receptor: 3.7%, 0.31 ng/ml.

Ding N., Karvonen-Gutierrez C.A., Herman W.H., Calafat A.M., Mukherjee B, & Park S.K. (2021). Associations of Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS) and PFAS Mixtures with Adipokines in Midlife Women. International journal of hygiene and environmental health, 235, 113777.

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Adipokine and Metabolic Biomarker Assays

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Fetuin-A (Quantikine; R&D Systems, Minneapolis, MN), leptin, HMW adiponectin, TNF-α (Millipore, Billerica, MA), and hs-CRP (Calbiotech, Spring Valley, CA) were assessed by enzyme-linked immunosorbent assay. Glucose kinetics, triglycerides, cholesterol, glucose, insulin, and NEFA were assessed by standard assays, as previously described (10 (link),13 (link)).

Malin S.K., Rincon J.P., Huang H, & Kirwan J.P. (2014). Exercise-Induced Lowering of Fetuin-A May Increase Hepatic Insulin Sensitivity. Medicine and science in sports and exercise, 46(11), 2085-2090.

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Adiponectin, Leptin, and sOB-R Assay Protocol

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HMW adiponectin, leptin, and sOB-R were assayed in duplicate at the University of Michigan using 12-hour fasted serum samples collected at the sixth SWAN follow-up visit (2002–2003). The assays used commercially available colorimetric enzyme immunoassay kits (leptin, adiponectin, and HMW adiponectin, Millipore, St. Charles, MO and sOB-R, R&D systems, Minneapolis, MN). The mean coefficient of variation for duplicate samples for each participant and lower limit of detection, respectively, were 4.0%, 0.78 ng/mL for adiponectin; 8.1%, 0.5 ng/ml for HMW adiponectin; 4.0%, 0.5 ng/mL for leptin; and 3.7%, 0.31 ng/ml for sOB-R.

Wang X., Karvonen-Gutierrez C.A., Mukherjee B., Herman W.H, & Kyun Park S. (2020). Urinary Metals and Adipokines in Midlife Women: The Study of Women’s Health Across the Nation (SWAN). Environmental research, 196, 110426.

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Cytokine and Antioxidant Biomarker Assay

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Inflammatory cytokines, including interleukin (IL)6, IL1R, IL8, IL10, monocyte chemotactic protein (MCP)1, IL15, interferon (IFN) gamma, and tumor necrosis factor (TNF) alpha, were measured using Milliplex MAP MAG Human Cytokine kit (Merck, Johannesburg, South Africa) and xMAP technology (Luminex, Austin, Texas) according to the manufacturer’s instruction. Serum concentrations of leptin and high molecular weight (HMW) adiponectin (EMD Millipore Corporation, St Charles, Missouri, USA) were analyzed using commercially available ELISA kits according to the manufacturer’s protocols. High-sensitive C-reactive protein (CRP) was measured by an immunochemiluminometric assay (IMMULITE 1000 immunoassay system, Siemens Healthcare, Midrand, South Africa). Lipid peroxidation was assessed by measuring the concentration of thiobarbituric acid reactive substance (TBARS); antioxidant capacity was assessed by measuring oxygen radical capacity absorbance (ORAC), as well as catalase and superoxide dismutase (SOD) activities as described previously [60 (link)].

Goedecke J.H., Mendham A.E., Clamp L., Nono Nankam P.A., Fortuin-de Smidt M.C., Phiri L., Micklesfield L.K., Keswell D., Woudberg N.J., Lecour S., Alhamud A., Kaba M., Lutomia F.M., van Jaarsveld P.J., de Villiers A., Kahn S.E., Chorell E., Hauksson J, & Olsson T. (2018). An Exercise Intervention to Unravel the Mechanisms Underlying Insulin Resistance in a Cohort of Black South African Women: Protocol for a Randomized Controlled Trial and Baseline Characteristics of Participants. JMIR Research Protocols, 7(4), e75.

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Comprehensive Metabolic and Inflammatory Profile

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A complete anthropometrical, biochemical, and physical examination was carried out on each patient. Body height and weight were measured with the patient standing in light clothes and shoeless. Body mass index was calculated as body weight divided by height squared (kg/m²). The subjects’ waist circumference was measured with a soft tape midway between the lowest rib and the iliac crest. Laboratory studies included glucose, insulin, glycated haemoglobin, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglycerides and transaminases, all of which were analysed using a conventional automated analyser. Insulin resistance was estimated using homeostasis model assessment of IR (HOMA2-IR) [33 (link)].
We determined the circulating levels of several molecules related to inflammation including adipokines (HMW adiponectin), acute phase proteins (CRP) and proinflammatory cytokines (IL6, TNFRI and TNFRII). Circulating levels of HMW adiponectin (EMD Millipore, St. Charles, MO, USA), CRP (Dade Behring, Marburg, Germany), IL6 (Quantikine, R&D Systems, Minneapolis, MN, USA), FABP4 (Biovendor, Modrice, Czech Republic), TNFRI and TNFRII (AssayPro, St. Charles, MO, USA) were measured in duplicate using enzyme-linked immunosorbent assays (ELISA) following the manufacturer’s instructions.

Auguet T., Berlanga A., Guiu-Jurado E., Martinez S., Porras J.A., Aragonès G., Sabench F., Hernandez M., Aguilar C., Sirvent J.J., Del Castillo D, & Richart C. (2014). Altered Fatty Acid Metabolism-Related Gene Expression in Liver from Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease. International Journal of Molecular Sciences, 15(12), 22173-22187.

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