Human rat beta amyloid 42 elisa kit

The secretion of Aβ into the cell culture medium was analyzed with a Human/Rat Beta Amyloid (40) ELISA Kit and Human/Rat Beta Amyloid (42) ELISA Kit (294-64701, 290-62601, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) as previously described [11 (link)]. The results are presented as percentages in comparison with control culture values.

Levels of Aβ42 in conditioned medium of H4‐APPswe were determined with the Human/Rat beta‐Amyloid (42) ELISA Kit, High Sensitivity from Wako Chemicals USA, Inc. Medium‐containing human recombinant Aβ42, collected at 6 and 20 h incubation, was diluted with the provided sample buffer immediately prior to the assay. Levels of BDNF in neuronal culture supernatants were measured with Rat BDNF ELISA kit Thermofisher.

The cerebral cortex and hippocampus tissues of PAP mice were homogenized in radio immunoprecipitation assay lysis buffer (P0013B, Beyotime, China) containing protease inhibitors (ST506, Beyotime, China), sonicated briefly, lysed on ice for 30 min with gentle agitation, and then centrifuged at 14,800 × g for 20 min at 4°C to remove debris. Aliquots of the lysates were tested for Aβ_1-42 monomers and Aβ oligomers, using the Human/Rat Beta Amyloid (42) ELISA kit (290–62601, Wako, Japan) and Mouse Aβ Oligomer ELISA kit (AB6094, Abmart, China), respectively, according to the manufacturer’s protocols. Plasma and brain tissue IL–1β and TNF–α were determined using the Mouse IL-1β (EMC001b, NeoBioscience, China) and TNF-α (EMC102a, NeoBioscience, China) ELISA kits in accordance with the manufacturer’s protocols. In addition, feces of PAP mice were homogenized at 50 mg/mL in sterilized PBS, and large debris were removed by brief pulsed centrifugation. The fecal suspension was centrifuged at 14,800 × g for 15 min at 4°C to remove sediments, and the levels of fecal LCN2 and albumin were assayed using the Mouse LCN-2/neutrophil gelatinase-associated lipocalin (NGAL) Quantikine ELISA kit (MLCN20, R&D Systems, USA) and Mouse Albumin ELISA kit (E13878m, Cusabio, China).