Mouse monoclonal anti α tubulin igg

Manufactured by Merck Group

Sourced in United States

Mouse monoclonal anti-α-tubulin IgG is a laboratory reagent used for the detection and identification of the α-tubulin protein, a major component of the cytoskeleton in eukaryotic cells.

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Lab products found in correlation

Sulforhodamine b, by Merck Group (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Mouse monoclonal anti βactin igg, by Merck Group (2 mentions) Rabbit monoclonalanti γ h2ax igg, by Cell Signaling Technology (2 mentions) Hoechst 33258, by Merck Group (2 mentions) Propidium iodide, by Merck Group (2 mentions) Colchicine, by Merck Group (2 mentions) Phosphate buffered saline (pbs), by HiMedia (2 mentions) Taxol, by Merck Group (2 mentions) Alkaline phosphatase conjugated anti mouse igg, by Merck Group (2 mentions) Vinblastine sulfate, by Merck Group (1 mentions) Fluorescein isothiocyanate fitc conjugated anti mouse igg, by Merck Group (1 mentions) Horseradish peroxidase conjugated goat anti rabbit igg, by Bio-Rad (1 mentions) Horseradish peroxidase conjugated horse anti mouse igg, by Cell Signaling Technology (1 mentions) Fbs fetal bovine serum, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Hydroxylamine hydrochloride, by Merck Group (1 mentions) Vinblastine sulphate, by Merck Group (1 mentions) Podophyllotoxin, by Merck Group (1 mentions) Fitc conjugated anti mouse igg, by Merck Group (1 mentions)

Close spelling variants of this product

Mouse monoclonal anti-α-tubulin Monoclonal anti-α-tubulin Mouse monoclonal anti-tubulin Mouse anti-α-tubulin Mouse α-tubulin Anti-α-tubulin Mouse monoclonal IgG Mouse anti-tubulin α-tubulin Anti-IgG mouse

6 protocols using mouse monoclonal anti α tubulin igg

Cytotoxicity Assays and Molecular Markers

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Vinblastine sulfate, propidium iodide, sulforhodamine
B (SRB),
mouse monoclonal anti-α tubulin IgG, mouse monoclonal anti-β
actin IgG, fluorescein isothiocyanate (FITC)-conjugated antimouse
IgG, Hoechst 33258, and bovine serum albumin (BSA) were purchased
from Sigma (St. Louis, MO, USA). Alexa flour 555 conjugated goat antirabbit,
alexa flour 594 conjugated goat antirabbit IgG, and fetal bovine serum
(FBS) were purchased from Molecular probes, Invitrogen (Eugene,
OR, USA). Rabbit polyclonal anti-PARP-1 IgG was purchased
from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Horseradish peroxidase conjugated horse antimouse IgG and rabbit monoclonal
anti-γ-H2AX IgG were purchased from Cell Signaling Technologies.
Horseradish peroxidase conjugated goat antirabbit IgG was purchased
from Biorad (USA). Supersignal
west picochemiluminescent substrate was purchased from
Thermofisher Scientific. All other reagents were of analytical grade
and were purchased from HiMedia (Mumbai, India) and Sigma.

Hura N., Naaz A., Prassanawar S.S., Guchhait S.K, & Panda D. (2018). Drug-Clinical Agent Molecular Hybrid: Synthesis of Diaryl(trifluoromethyl)pyrazoles as Tubulin Targeting Anticancer Agents. ACS Omega, 3(2), 1955-1969.

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Cytoskeleton Disruption Assay with Small Molecules

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Paclitaxel, vinblastine sulphate, podophyllotoxin, colchicine, 5,5′‐dithiobis‐2‐nitrobenzoic acid (DTNB), sulforhodamine B (SRB), Hoechst 33342, guanosine 5′‐triphosphate (GTP), propidium iodide, EGTA, MgCl₂, piperazine‐N,N′‐bis (2‐ethanesulphonic acid) (PIPES), mouse monoclonal anti‐α‐tubulin IgG and FITC‐conjugated anti‐mouse IgG, fluorescein isothiocyanate isomer (FITC) and dimethylformamide were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Foetal bovine serum (FBS) and Alexa Fluor 568‐conjugated anti‐mouse IgG were purchased from Invitrogen (Thermo Scientific, Massachusetts, USA). Acridine orange (AO), hydroxysuccinimide, minimal essential medium (MEM), cell culture tested antibiotic solution, and phosphate‐buffered saline (PBS) were purchased from HiMedia (Mumbai, India). Dichloromethane, n‐hexane, hydroxylamine hydrochloride and triethylamine were purchased from Merck, India. 1‐Ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride was purchased from SRL chemicals. All other reagents used in the study were of analytical grade.

Ashraf S.M., Sebastian J, & Rathinasamy K. (2018). Zerumbone, a cyclic sesquiterpene, exerts antimitotic activity in HeLa cells through tubulin binding and exhibits synergistic activity with vinblastine and paclitaxel. Cell Proliferation, 52(2), e12558.

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Western Blot Analysis of Myotubes

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Western blot analysis of treated vs. untreated myotubes (from both normal and DM1 diseased cell lines), was carried out using the following primary antibodies: mouse monoclonal anti-human hnRNP L IgG clone 4D11 (1:8000, Sigma-Aldrich, Cat# R4903); mouse monoclonal anti-human MBNL1 IgG (1:100, Developmental Studies Hybridoma Bank, Iowa University, Cat#MB1a(4A8)); as well as rabbit polyclonal anti-human GAPDH IgG (1:1000, Santa Cruz Biotechnology, Cat#sc-25778), and mouse monoclonal anti-α-Tubulin IgG (1:500, Sigma-Aldrich,Cat#T9026); the last two detect corresponding housekeeping (control) proteins.

Alexander M.S., Hightower R.M., Reid A.L., Bennett A.H., Iyer L., Slonim D.K., Saha M., Kawahara G., Kunkel L.M., Kopin A.S., Gupta V.A., Kang P.B, & Draper I. (2021). hnRNP L is essential for myogenic differentiation and modulates myotonic dystrophy pathologies. Muscle & nerve.

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Cytotoxicity and Oxidative Stress Assays

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Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone),
2′, 7′–dichlorofluorescein diacetate (DCFDA),
PI, NAC, mouse monoclonal anti-α-tubulin IgG, GTP, taxol, colchicine,
sulforhodamine B, Hoechst 33258, and rhodamine 123 dye were purchased
from Sigma-Aldrich (USA). Copper chloride was procured from Merck
(Germany). Anti-mouse Alexa 594 and fetal bovine serum (FBS) were
purchased from Thermo Fisher Scientific (USA). Rabbit monoclonal anti-γ-H2AX
IgG was purchased from Cell Signaling Technologies (USA). Dulbecco’s
Modified Eagle’s medium (DMEM), antibiotic-antimycotic solution,
and phosphate-buffered saline (PBS) were purchased from HiMedia (India).
Sodium methoxide, methanol, and DMSO were obtained from Merck. All
other reagents were procured either from Merck or HiMedia.

Mukherjee S., Sawant A.V., Prassanawar S.S, & Panda D. (2023). Copper-Plumbagin Complex Produces Potent Anticancer Effects by Depolymerizing Microtubules and Inducing Reactive Oxygen Species and DNA Damage. ACS Omega, 8(3), 3221-3235.

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Purification of Recombinant Proteins

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Taxol, bovine thrombin, GTP (guanosine triphosphate), mouse monoclonal anti-α-tubulin IgG, FITC (fluorescein isothiocyanate) conjugated anti-rabbit IgG, Alexa-568 conjugated anti-mouse IgG, alkaline phosphatase conjugated anti-mouse IgG and anti-rabbit IgG were procured from Sigma-Aldrich. Rabbit polyclonal anti-MAP7D3 IgG was obtained from Abcam. Isopropyl β-D-1-thiogalactopyranoside (IPTG) was obtained from Calbiochem and Ni-NTA agarose resin was obtained from Qiagen. All the other reagents used were of analytical grade and were from Sigma, Merck Millipore and Himedia (Mumbai, India) brands.

Yadav S., Verma P.J, & Panda D. (2014). C-Terminal Region of MAP7 Domain Containing Protein 3 (MAP7D3) Promotes Microtubule Polymerization by Binding at the C-Terminal Tail of Tubulin. PLoS ONE, 9(6), e99539.

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Apoptosis Assay Using Molecular Probes

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Sulforhodamine B (SRB), mouse monoclonal anti-α-tubulin IgG, mouse monoclonal anti-β-actin IgG, alkaline phosphatase conjugated anti-mouse IgG, rabbit monoclonal anti-Bax IgG, alkaline phosphatase conjugated anti-rabbit IgG, Hoechst 33258 dyes were purchased from Sigma. Annexin V FITC and propidium iodide apoptosis detection kit was purchased from BD Pharmigen. Alexa flour 568 anti-mouse IgG and FBS were purchased from Molecular probes, Invitrogen. Mouse monoclonal anti-p53 IgG, rabbit polyclonal anti-Bcl2 IgG, rabbit polyclonal anti-PARP (poly ADP ribose polymerase) IgG and mouse monoclonal anti-p21 IgG were purchased from Santa Cruz Biotechnology. Rabbit polyclonal anti-murine double minute 2 (Mdm2; S166) was purchased from Abcam. ¹H NMR was recorded on a Buker 300 Hz instrument and mass on Applied Biosystem 4700. Other reagents used in the study were of analytical grade and were obtained from Sigma or HiMedia.

Srivastava S., Mishra S., Surolia A, & Panda D. (2016). C1, a highly potent novel curcumin derivative, binds to tubulin, disrupts microtubule network and induces apoptosis. Bioscience Reports, 36(2), e00323.

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