Precicontrol clinchem multi 1

Glucose, lactate and total protein concentrations were measured on Cobas 6000 c501 biochemistry analyser (Roche Diagnostics GmbH, Mannheim, Germany) with standard methods, using reagents GLUC3 (enzymatic reference method with hexokinase), LACT2 (enzymatic method with lactate oxidase) and TPUC3 (precipitating method with benzethonium chloride), respectively (Roche Diagnostics GmbH, Mannheim, Germany). For each analyte, quality control was performed daily in two levels using PreciControl ClinChem Multi 1 and PreciControl ClinChem Multi 2 (Roche Diagnostics GmbH, Mannheim, Germany), and Precinorm PUC and Precipath PUC controls (Roche Diagnostics GmbH, Mannheim, Germany). Coefficients of variations in the tested time period were within analytical requirements for imprecision.

Internal quality control (IQC) results from October 2019 to December 2019 were used for our laboratory’s coefficient of variation (CV%) calculation. PreciControl Clin Chem Multi 1 and Multi 2 (level 1 lot no:250280, level 2 lot no: 324196, Roche Diagnostic GmbH, Mannheim, Germany) IQC materials were carried out two times per day. Bias estimation of all analytes were determined by twelve months of data obtained during a cycle from the external quality control program (KBUDEK, Turkey) between January 2019 and December 2019. Bias calculation was based on peer-group values using the same device and method in the external quality control (EQC) program. Among the evaluated EQC results, for all analytes, only 8 results were found outside the acceptable limits during one cycle, and were excluded from the calculation of bias, and the overall EQC success was above 95%. Regulatory-preventive action was initiated for each excluded result, and the sequent EQC result was found within acceptable limits.