75 cm2 flasks

Three human colorectal carcinoma cell lines were used for the studies, viz. SW480 and an isogenic pair of HCT116 cell lines (the parental or wild type line and a subline with acquired resistance to oxaliplatin, resistance factor >20-fold). All cells lines were kindly provided by the Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Austria, authenticated and confirmed to be mycoplasma and cross-contamination free (Multiplexion, Germany, 2014). All cell lines were grown in RPMI 1640 medium (Sigma Aldrich) supplemented with 10% fetal bovine serum (Biowest) and 4 mM l-glutamine (Sigma Aldrich), referred to as complete RPMI 1640 medium below. Monolayer cell cultures were grown in 75 cm² flasks (Starlab) at 37 °C in a moist atmosphere containing 5% CO₂ in air.

The human colon carcinoma cell line HCT116 was kindly provided by Brigitte Marian, Institute of Cancer Research, Department of Medicine I, Medical University of Vienna. HCT116 cells were cultured as adherent monolayers in 75 cm² flasks (StarLab), McCoy’s 5a medium (Sigma-Aldrich) was used supplemented with 4 mM l-glutamine and 10% fetal calf serum (FCS) (BioWest) without antibiotics at 37 °C under a humidified atmosphere containing 5% CO₂. Cell culture media and reagents were obtained from Sigma-Aldrich, and all plastic dishes, plates and flasks were from StarLab unless stated otherwise.

CH1/PA-1 (ovarian teratocarcinoma) cells were a generous gift from Lloyd R. Kelland (CRC Center for Cancer Therapeutics, Institute of Cancer Research, Sutton, UK). A549 and SW480 cells were kindly provided by the Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Austria. All cell lines were grown as monolayer cultures in Eagle's minimal essential medium (MEM) supplemented with l-glutamine (4 mM), sodium pyruvate (1 mM), and 1% (v/v) non-essential amino acid solution (all from Sigma-Aldrich) and 10% (v/v) heat-inactivated fetal calf serum (FCS, from BioWest) in 75 cm² flasks (Starlab) at 37°C under a humidified atmosphere containing 5% CO₂ in air.