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Mgammag 300k

Manufactured by Merck Group
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The MGAMMAG-300K is a laboratory equipment product manufactured by Merck Group. It is a high-performance magnetic separator designed for the efficient separation and purification of magnetic particles. The core function of the MGAMMAG-300K is to provide a powerful magnetic field for the effective separation of magnetic particles from liquid samples.

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Lab products found in correlation

Mcytomag 70k, by Merck Group (4 mentions) Luminex 200, by DiaSorin (2 mentions) Magpix multiplex reader, by Bio-Rad (1 mentions) Mip 1β, by Merck Group (1 mentions) Tnf β, by Merck Group (1 mentions) Il 23, by Merck Group (1 mentions) Bio plex pro mouse chemokine panel, by Bio-Rad (1 mentions) Nanodrop 8000, by Thermo Fisher Scientific (1 mentions) Bradford assay, by Bio-Rad (1 mentions)

11 protocols using mgammag 300k

1

Autoantibody and Immunoglobulin Subclass Analysis

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Autoantibodies (dsDNA) and immunoglobulin subclasses were measured with kits from Alpha Diagnostic International (5110) and Millipore (MGAMMAG-300K), respectively.
Shrestha S., Yang K., Guy C., Vogel P., Neale G, & Chi H. (2015). Regulatory T cells require the phosphatase PTEN to restrain type 1 and follicular helper T-cell responses. Nature immunology, 16(2), 178-187.
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2

Magnetic Bead-Based Ig Isotyping

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Antibody Ig isotyping was performed using a magnetic bead-based multiplex assay (Millipore Cat: MGAMMAG-300K). The assay was performed according to the manufacturer’s instructions. The results were obtained by reading the plates on the Bioplex MAGPIX Multiplex Reader, and the concentration was calculated by using Bio-plex manager 6.1 software. A total of 6 markers (IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM) were tested and quantified for each standard.
Fihurka O., Hong Y., Yan J., Brown B., Lin X., Shen N., Wang Y., Zhao H., Gordon M.N., Morgan D., Zhou Q., Chang P, & Cao C. (2022). The Memory Benefit to Aged APP/PS1 Mice from Long-Term Intranasal Treatment of Low-Dose THC. International Journal of Molecular Sciences, 23(8), 4253.
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3

Multiplex Analysis of Cytokine Profiles

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Plasma samples of the mice were analyzed using multiplex bead analysis. The manufacturer’s instructions were followed to measure interleukin (IL)-1β, IL-2, IL-5, IL-17, IL-6, IL-4, IL-10, granulocyte-macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), interferon (IFN)-γ, tumor necrosis factor (TNF)-α, monocyte chemotactic protein (MCP)-1, regulated upon activation normal T cell expressed and secreted factor (RANTES), eotaxin, macrophage inflammatory protein (MIP)-1β, IL-23, and TNF-β (Millipore Corp., Billerica, MA, USA). The samples were analyzed using Luminex 200™ (Luminex, Austin, TX, USA). The levels of IL-1β, IL-2, IL-5, IL-17, IL-6, IL-4, IL-10, GM-CSF, G-CSF, IFN-γ, TNF-α, MCP-1, RANTES, eotaxin, and MIP-1β were detected using a multiplex map kit (MCYTOMAG-70 K, Millipore). IL-23 and TNF-β were detected using another multiplex map kit (MGAMMAG-300 K, Millipore).
Wang J.H., Lei X., Cheng X.R., Zhang X.R., Liu G., Cheng J.P., Xu Y.R., Zeng J., Zhou W.X, & Zhang Y.X. (2016). LW-AFC, a new formula derived from Liuwei Dihuang decoction, ameliorates behavioral and pathological deterioration via modulating the neuroendocrine-immune system in PrP-hAβPPswe/PS1ΔE9 transgenic mice. Alzheimer's Research & Therapy, 8, 57.
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4

Autoantibody and Immunoglobulin Subclass Analysis

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Autoantibodies (dsDNA) and immunoglobulin subclasses were measured with kits from Alpha Diagnostic International (5110) and Millipore (MGAMMAG-300K), respectively.
Shrestha S., Yang K., Guy C., Vogel P., Neale G, & Chi H. (2015). Regulatory T cells require the phosphatase PTEN to restrain type 1 and follicular helper T-cell responses. Nature immunology, 16(2), 178-187.
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5

Measuring Immunoglobulin Subclasses

Cited 1 time
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Immunoglobulin subclasses were measured with kits from Alpha Diagnostic International (5110) and Millipore (MGAMMAG-300K), respectively36 (link).
Yang K., Blanco D.B., Neale G., Vogel P., Avila J., Clish C.B., Wu C., Shrestha S., Sherri R., Long L., Anil K, & Chi H. (2017). Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signaling. Nature, 548(7669), 602-606.
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6

Multiplex Cytokine Profiling in Mice

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Plasma samples of the mice were diluted 1:1 in the assay buffer and analyzed by multiplex bead analysis. Interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-5 (IL-5), interleukin-17 (IL-17), interleukin-6 (IL-6), interleukin-4 (IL-4), interleukin-10 (IL-10), granulocyte-macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), interferon-γ (INF-γ), tumor necrosis factor α (TNF-α), monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted factor (RANTES), eotaxin, macrophage Inflammatory Protein-1β (MIP-1β), interleukin-23 (IL-23) and tumor necrosis factor β (TNF-β) were measured according to the manufacturer's instructions (Millipore Corp., USA). Briefly, the plasma samples were incubated with premixed beads overnight at 4°C, washed beads were further incurred with detection antibody for 1 h at room temperature followed by incubation with streptavidin-phycoerythrin for 30 min at room temperature. The samples were analyzed by Luminex 200™ (Luminex, Austin, TX, USA). The levels of IL-1β, IL-2, IL-5, IL-17, IL-6, IL-4, IL-10, GM-CSF, G-CSF, INF-γ, TNF-α, MCP-1, RANTES, Eotaxin, MIP-1β were detected by a multiplex map kit (MCYTOMAG-70K, Millipore),and IL-23, TNF-β were detected by another multiplex map kit (MGAMMAG-300K, Millipore).
Wang J., Cheng X., Zhang X., Cheng J., Xu Y., Zeng J., Zhou W, & Zhang Y. (2016). The anti-aging effects of LW-AFC via correcting immune dysfunctions in senescence accelerated mouse resistant 1 (SAMR1) strain. Oncotarget, 7(19), 26949-26965.
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7

Measuring Immunoglobulin Subclasses

Cited 1 time
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Immunoglobulin subclasses were measured with kits from Alpha Diagnostic International (5110) and Millipore (MGAMMAG-300K), respectively36 (link).
Yang K., Blanco D.B., Neale G., Vogel P., Avila J., Clish C.B., Wu C., Shrestha S., Sherri R., Long L., Anil K, & Chi H. (2017). Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signaling. Nature, 548(7669), 602-606.
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8

Immunoglobulin Titration in NP-CGG Mice

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To assess immunoglobulin titers of NP-CGG immunized mice, serum was collected one week prior to injection and 14 days after NP-CGG treatment. Luminex multiplex assays were performed using the mouse Ig isotyping kit (MGAMMAG-300K, Millipore, Burlington MA) and conducted according to the manufacturer’s instructions by the Analytical Pharmacology Core at City of Hope. The displayed data are calculated by subtracting the baseline serum levels from the post-immunization serum to reflect change (delta) in serum levels upon immunization.
Lindner S.E., Egelston C.A., Huard S.M., Lee P.P, & Wang L.D. (2020). Arhgap25 deficiency leads to decreased numbers of peripheral blood B cells and defective germinal center reactions. ImmunoHorizons, 4(5), 274-281.
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9

Quantifying Antibody Titers in NP-CGG Mice

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To assess immunoglobulin titers of NP-CGG immunized mice, serum was collected one week prior to injection and 14 days after NP-CGG treatment. Luminex multiplex assays were performed using the mouse Ig isotyping kit (MGAMMAG-300K, Millipore, Burlington MA) and conducted according to the manufacturer's instructions by the Analytical Pharmacology Core at City of Hope.
Lindner S.E., Egelston C.A., Huard S.M., Lee P.P., & Wang L.D. (2020). Arhgap25 deficiency leads to decreased numbers of peripheral blood B cells and defective germinal center reactions.
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10

Multiplex Cytokine Profiling in BALF

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Cell-free BALF was assayed for keratinocyte chemokine (KC/CXCL1), macrophage inflammatory protein 2 (MIP-2 /CXCL2), granulocyte-colony stimulating factor (G-CSF), monocyte chemokine protein-1(MCP-1/CCL2), macrophage inflammatory protein 1-alpha (MIP-1α/CCL3), macrophage inflammatory protein 1-beta (MIP-1β/CCL4), IL-4, IL-5, IL-6, IL-13, IL-17, IP-10, IL-1α, IL-1β, and IL-10 using Luminex-XMAP-based assay (MCYTOMAG-70K) according to the manufacturer’s instructions (EMD Millipore, Billerica, MA). To assess the levels of eosinophilic inflammation relevant cytokines, i.e., Eotaxin, CCL7, CCL12, CCL17, CCL22, CCL24 (BioPlex Pro Mouse Chemokine panel, Hercules, CA), we used lung homogenates because of the unavailability of enough BALF sample. The data were normalized to the total protein concentration. Total protein contents in the BALF were determined by Bradford assay (Bio-Rad, Hercules, CA). Total dsDNA contents were determined spectrophotometrically, using Nanodrop 8000 (Thermo Scientific, Waltham, MA). Immunoglobulin concentrations in the cell-free BALF were determined with MILLIPLEX MAP mouse immunoglobulin isotyping magnetic bead panel isotyping multiplex assay (MGAMMAG-300K) according to the manufacturer’s instructions (EMD Millipore, Billerica, MA).
Choudhary I., Vo T., Paudel K., Yadav R., Mao Y., Patial S, & Saini Y. (2021). Postnatal ozone exposure disrupts alveolar development, exaggerates mucoinflammatory responses, and suppresses bacterial clearance in developing Scnn1b-Tg+ mice lungs. Journal of immunology (Baltimore, Md. : 1950), 207(4), 1165-1179.
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