Waters xevo g2 xs qtof high resolution mass spectrometer

Foc TR4 was inoculated on the PDB liquid medium and cultured for 3 d. Strain Y1-14 extracts were dissolved in 10% (v/v) of DMSO with the final concentration 1 × EC₅₀. The same concentration of DMSO was used as a negative control. The samples were collected after treatment at 0 h, 6 h, and 12 h. The spore solution was filtered through two layers of filter paper to collect the treated mycelia. The materials were sent to the Biomarker Technology Company (Beijing, China) for metabolomics analysis.
The LC/MS system for metabolomics analysis was composed of Waters Acquity I-Class PLUS ultra-high performance liquid tandem Waters Xevo G2-XS QT high-resolution mass spectrometer. Acquity UPLC HSS T3 column (1.8 µm × 2.1 mm × 100 mm) was purchased from Waters (Waters Corporation, Milford, MA, USA). Waters Xevo G2-XS QTOF high-resolution mass spectrometer was used to collect primary and secondary mass spectrometry data in MSe mode (MassLynx V4.2, Waters). MassLynx V4.2 was used to collect the raw data, and Progenesis QI software (Waters, Wilmslow, UK) was used for peak extraction, peak alignment, and other data processing operations. Progenesis QI software online METLIN database and Biomark’s self-built library were used for identification and theoretical fragment identification. Quality deviation fell within 100 ppm.

Millet extracts were analyzed using an ACQUITY UPLC I-Class PLUS system (Waters Corporation, Milford, MA, United States) coupled to a Xevo G2-XS QTof system (Waters Corporation, Milford, MA, United States) with an electrospray ionization (ESI) source under positive and negative modes. In the ESI+ and ESI− modes, mobile phases A and B were 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile, respectively. The injection volume was 1 μL. Chromatographic separation was achieved using an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm; Waters Pacific Pte. Ltd, Milford, MA, United States) at a flow rate of 400 μL/min using the following gradient: 98% A and 2% B for 0.25 min, decreased to 2% A and increased to 98% B within 13 min, and then increased to 98% A and decreased to 2% B in the last 2 min.
A Waters Xevo G2-XS QTOF high-resolution mass spectrometer can collect primary and secondary mass spectrometry data in the MSe mode under the control of the acquisition software (MassLynx V4.2, Waters Corporation, Milford, MA, United States). In each data acquisition cycle, it can simultaneously conduct dual-channel data acquisition for low and high collision energies. The low collision energy was 2 V, the high collision energy range was 10–40 V, and the scanning frequency was 0.2 s.