Anti caspase 2

Doxorubicin was purchased from Biotrend Chemicals (Cologne, Germany). Actinomycin D and rapamycin were obtained from Calbiochem (Schwalbach, Germany) and Merck KGaA (Darmstadt, Germany). The caspase-2-specific chemical inhibitor (Z-VDVAD-FMK) was from R & D Systems (Wiesbaden, Germany). Antibodies used in this study included the following: Anti-TRIM25 (#115737) from Abcam (Berlin, Germany) and (sc-166926) from Santa Cruz (Heidelberg, Germany), respectively. Anti-caspase-2 (#611022) from BD Biosciences (Heidelberg, Germany), anti-caspase-3 (#9662), anti-phospho-S6 ribosomal protein (Ser240/244, #2215), and anti-cytochrome c (#4272) from Cell Signaling (Frankfurt, Germany), anti-β-actin (#A2228) were from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Secondary Horseradish Peroxidase HRP-linked antibodies sc-2054 and sc-20559 were obtained from Santa Cruz and a Cy5-coupled goat anti-mouse antibody was derived from Life Technologies (Darmstadt, Germany). Hyperfilm and the ECL system were purchased from GE Healthcare (Freiburg, Germany). Go-Taq polymerase was from Promega (Mannheim, Germany). Medium and supplements as well as modifying enzymes were from Invitrogen (Karlsruhe, Germany).

All cell culture media, supplements and modifying enzymes were purchased from Invitrogen (Karlsruhe, Germany) if not otherwise indicated. Doxorubicin was from Biotrend Chemicals (Cologne, Germany), paclitaxel, and propidium iodide were obtained from Sigma-Aldrich (Taufkirchen, Germany). Gö6983, KU55933, SB203580, U0126 and Chk2 inhibitor II were from Merck (Darmstadt, Germany). The caspase inhibitors Z-VDVAD-FMK and Z-VAD-FMK were derived from R & D Systems (Wiesbaden, Germany). The following antibodies were used in this study: anti-HuR (sc-5261) and anti-Lamin B1 (sc-20682) from Santa Cruz (Heidelberg, Germany), anti-caspase-2 (#611022, BD Biosciences, Heidelberg, Germany), anti-caspase-3 (#9662), anti-caspase-7 (#9494) anti-PARP (#9542) and anti-Bid (#2002) antibodies were from Cell Signaling (Frankfurt, Germany), and anti-β-actin (#A2228) from Sigma-Aldrich. The goat anti-rabbit (sc-2054) and goat anti-mouse (sc-20559) HRP-linked antibodies were from Santa Cruz and Alexa Fluor 488 goat anti-mouse was from Life Technologies (Darmstadt, Germany). Radionucleotides were from Perkin Elmer (Rodgau, Germany) and the ECL system and Hyperfilms were from GE Healthcare (Freiburg, Germany).