Proteins were prepared and immunoblotting was performed as described previously24 (link). Proteins from the cells and tissues were separated by SDS-PAGE and transferred onto nitrocellulose membranes. The membranes were incubated with antibodies against ACC1 and FAS (Cell Signaling Technology), SREBP-1 kindly provided by Dr. Timothy F. Osborne (Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, USA), and β-actin (Santa Cruz Biotechnology), and developed using an enhanced chemiluminescent western blot detection kit (Amersham Bioscience, Piscataway, NJ, USA). Band intensities below the saturation threshold were measured using ImageJ software. Values are expressed as the integrals of each band.
Enhanced chemiluminescent western blot detection kit
Manufactured by Cytiva
Sourced in United States
The Enhanced Chemiluminescent Western Blot Detection Kit is a laboratory equipment product designed for the detection of proteins using the western blot technique. The kit provides the necessary reagents and components to facilitate the visualization and analysis of target proteins on a western blot membrane.
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Lab products found in correlation
2 protocols using enhanced chemiluminescent western blot detection kit
1
Quantitative Western Blot Analysis
2
Western Blot Analysis of BMDM
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Mouse BMDM were isolated and processed according to a method described previously (33) (link). The membranes were probed with indicated antibodies, and then developed using an enhanced chemiluminescent Western blot detection kit (Amersham Bioscience, Piscataway, NJ, USA). The intensities of bands were calculated using ImageJ, verifying for non-saturation and subtracting background. Values are expressed as the integrals (target area density) of each band (normalized to total indicated protein band).
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