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The PRS416 is a compact, automated pipetting system designed for high-throughput liquid handling applications. It features 16 independent pipetting channels, allowing for simultaneous processing of multiple samples. The system is designed to provide accurate and precise liquid transfers with a wide volume range. Further details on the intended use or specific applications of this product are not available.

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Lab products found in correlation

Pfastbac1, by Thermo Fisher Scientific (1 mentions) Pgex4t, by Cytiva (1 mentions) Glutathione sepharose 4b beads, by GE Healthcare (1 mentions) M2 agarose, by Merck Group (1 mentions) Frozen ez yeast transformation 2, by Zymo Research (1 mentions)

2 protocols using prs416

1

Recombinant Protein Expression Protocols

Cited 2 times
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The cDNAs for yeast genes were PCR-amplified from yeast genomic DNA. For protein expression in yeast, cDNAs were subcloned into pRS416 (ATCC). For GST-tagged proteins, cDNAs were subcloned into pGEX4T (Amersham), expressed in Escherichia coli, and purified on Glutathione-Sepharose 4B beads (GE Healthcare). For baculovirus-mediated expression, cDNAs were subcloned into pFASTBAC1 (Gibco-Invitrogen) with or without an epitope tag and baculoviruses were generated according to the manufacturer's instruction (Gibco-Invitrogen). For expression of FLAG-tagged proteins, and for reconstitution of Set1 complexes containing FLAG-Set1 or FLAG-Set1 fragments, Sf9 cells were infected with combinations of baculoviruses and proteins/complexes were affinity purified on M2 agarose (Sigma) as described (21 (link)). The expression and purification of recombinant Xenopus histones, semi-synthetic H2Bub, histone octamers, NAP1 and the ACF complex were as described (20 (link),21 (link)).
Jeon J., McGinty R.K., Muir T.W., Kim J.A, & Kim J. (2018). Crosstalk among Set1 complex subunits involved in H2B ubiquitylation-dependent H3K4 methylation. Nucleic Acids Research, 46(21), 11129-11143.
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2

Genetic Engineering of Yeast Mitochondria

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All Saccharomyces cerevisiae strains used in this study were derivatives of BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0). The SDH1 (yeast homolog to SDHA) deletion strain (sdh1Δ) was purchased from ATCC (catalog #4004998). The sdh1Δ was constructed as part of the Saccharomyces Genome Deletion Project by homologous recombination using the KanMX4 cassette (22 (link)). We verified the deletion of SDH1 using PCR mapping of the SDH1 locus with primer pairs recommended by the Saccharomyces Genome Deletion Project.
An amplicon containing the WT SDH1 (including the native SDH1 promoter and 3′ UTR) was generated from WT BY4741 and cloned into the pRS416 plasmid (23 (link)) (ATCC) and expressed in the sdh1Δ strain. The SDH1 point mutations were introduced by QuikChange mutagenesis PCR system (Agilent Technology, cat #200521). All mutations were confirmed by Sanger sequencing. Yeast strains were transformed using Frozen EZ Yeast Transformation II (Zymo Research, catalog # T2001). Strains were grown in synthetic complete medium lacking uracil to maintain plasmid selection with either 2% glucose or 3% glycerol as the carbon source.
Bannon A.E., Kent J., Forquer I., Town A., Klug L.R., McCann K., Beadling C., Harismendy O., Sicklick J.K., Corless C., Shinde U, & Heinrich M.C. (2017). Biochemical, Molecular, and Clinical Characterization of Succinate Dehydrogenase Subunit A Variants of Unknown Significance. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(21), 6733-6743.
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