N acetyl l cysteine

18β-GA was obtained from J&K Scientific (Shanghai, China). Platelet-derived growth factor-beta polypeptide and N-acetyl-l-cysteine (NAC) were purchased from AbMole BioScience (Shanghai, China). The cell apoptosis detection kit was purchased from BD Biosciences (Franklin Lake, NJ, USA). Specific primary antibodies used were as follows: alpha-smooth muscle actin (α-SMA), PRDX1, PRDX2 (Abcam, Cambridge, UK), collagen type I alpha-1 (COL1α1), and vimentin (VIM) (Proteintech, Chicago, IL, USA). β-actin antibody was obtained from Affinity Biosciences (Changzhou, China).

Celastrol (purity ≥98%) was purchased from Bethealth People Biomedical Technology (Beijing, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Japan). N-Acetyl-l-cysteine and deferoxamine mesylate were obtained from AbMole BioScience (USA). Click chemistry reaction and LC–MS/MS reagents include: TAMRA-azide, Biotin-azide and THPTA (ClickChemistryTools, USA); NaVc and CuSO₄ (Sigma–Aldrich, USA); high capacity neutravidin agarose beads, TEAB and sequencing grade modified trypsin (Thermo, USA); Oasis HLB Extraction Cartridge (Waters, USA); Pierce™ Quantitative Fluorometric Peptide Assay Kit (Thermo, USA).
Specific primary antibodies anti-PRDX1, anti-PRDX2, anti-PRDX3, anti-PRDX4, anti-PRDX5, anti-PRDX6, anti-HO-1 (Abcam, UK), anti-Vimentin, anti-PPARγ, anti-COL1Al (Proteintech, China) and anti-β-actin (Affinity Biosciences, China) were used.