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Kif5a

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KIF5A is a protein that functions as a molecular motor, responsible for the transport of cargo within cells. It is a member of the kinesin family of proteins, which use energy from ATP hydrolysis to generate movement along microtubules.

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Lab products found in correlation

Enhanced chemiluminescence, by GE Healthcare (1 mentions) Gabab receptor 1, by Abcam (1 mentions) Gabaaδ subunits, by Rockland Immunochemicals (1 mentions) α tubulin, by Merck Group (1 mentions) Horseradish peroxidase conjugated anti mouse or anti rabbit antibodies, by GE Healthcare (1 mentions) Kif5b, by Abcam (1 mentions) Halt protease phosphatase inhibitor mixture, by Thermo Fisher Scientific (1 mentions) C fos, by Cell Signaling Technology (1 mentions) Total jnk, by Santa Cruz Biotechnology (1 mentions) Jip 1, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Calnexin, by Santa Cruz Biotechnology (1 mentions) Anti lc3 antibody, by Merck Group (1 mentions) Acetyl histone h3, by Cell Signaling Technology (1 mentions)

2 protocols using kif5a

1

Western Blot Analysis of Neuronal Proteins

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Proteins were transferred from gels onto nitrocellulose membranes followed by incubation with the following primary antibodies at room temperature for 2–3 h in 5% milk at the stated dilutions: Shrm4 (1:200, used previously4 (link)), GFP (1:2,000, MBL International), GABAAα1 (1:1,000, Millipore), HA (1:1,000, Invitrogen) and GABAB2 (1:1,000, gift of B.B.) KIF5A (1:1,000, Abcam), KIF5B (1:1,000, gift of F. Navone71 (link)) and KIF5C (1:1,000, Abcam) (all raised in rabbit); and: α-tubulin (1:40,000, Sigma), GABAB Receptor 1 (1:2,000, Abcam), GABAAδ subunits (1:500, ROCKLAND), V5 (1:1,000, Invitrogen), myc (1:2,000, MBL International), Synaptophysin (1:500, NeuroMab), DIC (1:1,000, Abcam) (raised in mouse); and GABAAγ2 (1:1,000, Abcam) raised in guinea pig. After rinsing, primary antibodies were revealed by incubation at room temperature for 1 h with horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibodies (both 1:2,000 from GE Healthcare) and immunoreactive bands on blots were visualized by enhanced chemiluminescence (GE Healthcare).
Zapata J., Moretto E., Hannan S., Murru L., Longatti A., Mazza D., Benedetti L., Fossati M., Heise C., Ponzoni L., Valnegri P., Braida D., Sala M., Francolini M., Hildebrand J., Kalscheuer V., Fanelli F., Sala C., Bettler B., Bassani S., Smart T.G, & Passafaro M. (2017). Epilepsy and intellectual disability linked protein Shrm4 interaction with GABABRs shapes inhibitory neurotransmission. Nature Communications, 8, 14536.
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2

Western Blot Analysis of Protein Signaling

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Cells were lysed in radioimmunoprecipitation assay buffer (RIPA) containing 50 mM Tris (pH 8.0), 150 mM NaCl, 0.1% SDS, 0.5% sodium deoxycholate, 1% NP-40 and HALT™ protease/phosphatase inhibitor mixture (Thermo Fisher). Cellular lysates were sonicated before clarification by centrifugation. Proteins were resolved by SDS-PAGE, transferred to nitrocellulose membranes (BioRad) and probed with antibodies for JIP1 (Santa Cruz Biotechnology, sc-25267), total JNK (Santa Cruz Biotechnology, sc-474), phospho-JNK (Cell Signaling Technology, 4668), cFos (Cell Signaling Technology, 4384), acetylhistone H3 (Cell Signaling Technology, 4499), KIF5A (Abcam, ab5268), calnexin (Santa Cruz Biotechnology, sc-11397), Myc (Santa Cruz Biotechnology, sc-40), and GAPDH (Invitrogen, AM4300). Immunoblotting with an anti-LC3 antibody (Sigma, L7543) was performed using PVDF membrane (BioRad).
Blakeslee W.W., Lin Y.H., Stratton M.S., Tatman P.D., Hu T., Ferguson B.S, & McKinsey T.A. (2017). Class I HDACs control a JIP1-dependent pathway for kinesin-microtubule binding in cardiomyocytes. Journal of molecular and cellular cardiology, 112, 74-82.
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