Vascular infiltration of scaffolds was determined using goat polyclonal anti-rat CD31 (Santa Cruz) which identifies endothelial cells. Formalin fixed samples were cut at 5 mm, dewaxed, rehydrated and antigen retrieval performed for CD31 by microwaving in 500 mL Tris and EDTA for 3 min. Sections were then incubated in 5% H2O2 in methanol to block endogenous peroxidase and incubated with the primary antibodies with normal rabbit or horse serum for 1 h at room temperature at a 1:300 dilution. The sections were then washed in PBS and incubated with biotinylated secondary stage antibody (rabbit anti goat) for 30 min and the reaction product was visualised by DAB. Sections were counterstained using haematoxylin.
Anti rat cd31
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-rat CD31 is a laboratory reagent designed for use in immunological research. It functions as an antibody that specifically recognizes the CD31 antigen present on the surface of rat cells. This product can be used for the identification and characterization of CD31-expressing cells in various rat-based experimental models.
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Lab products found in correlation
2 protocols using anti rat cd31
1
Vascular Infiltration of Scaffolds
2
Histomorphometric Analysis of Adipose Tissue
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After completion of all the CT examinations, each rat was perfused transcardially with phosphate-buffered saline, followed by freshly prepared 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). A portion of the visceral WAT and BAT in the interscapular region were fixed, dehydrated, embedded, and transversely sectioned into 5-µm-thick slices for hematoxylin and eosin (H&E) staining, as well as immunohistochemistry for UCP1, CD31, and alpha-smooth muscle actin (α-SMA). All histopathology slides were then examined, and fat content and UCP1 analyses were performed using the histological semi-automatic vacuole segmentation procedure (HIS-S) developed with the MATLAB software (The MathWorks, Inc., Natick, MA, USA) (18 (link)). A pathologist manually excluded artificial areas, such as blood vessels. The percentages of fat and UCP1 identified using HIS-S were calculated with the following formulae:
Fat content by HIS-S = area of fat / total tissue area
UCP1 content by HIS-S = the area of UCP1-positive zone / the total tissue area
The microvessel densities of WAT and BAT were calculated from serial sections stained with the anti-rat CD31 (Santa Cruz Biotechnology Inc, Dallas, TX, USA) antibody. Small vessels were identified with positive α-SMA staining.
Fat content by HIS-S = area of fat / total tissue area
UCP1 content by HIS-S = the area of UCP1-positive zone / the total tissue area
The microvessel densities of WAT and BAT were calculated from serial sections stained with the anti-rat CD31 (Santa Cruz Biotechnology Inc, Dallas, TX, USA) antibody. Small vessels were identified with positive α-SMA staining.
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