Electrophoresis reagents

Sodium dodecylsulfate (SDS) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Canada (Oakville, ON, Canada). Cell culture media were obtained from Life Technologies (Burlington, ON, Canada). Electrophoresis reagents were purchased from Bio-Rad (Mississauga, ON, Canada). The HyGLO chemiluminescent HRP antibody detection reagent was from Denville Scientific Inc. (Metuchen, NJ, USA). Micro bicinchoninic acid protein assay reagents were from Pierce (Rockford, IL, USA). The MMP inhibitor Ilomastat was from Merck Millipore (Etobicoke, ON, Canada), while Doxycycline and Actinonin were from Sigma-Aldrich Canada. Angiopep-2 (An2), Alexa⁴⁸⁸-An2, and Alexa⁵⁶⁸-An2 were a gift from Angiochem Inc (Montreal, QC, Canada). The monoclonal anti-MT1-MMP catalytic domain antibody 3G4.2 was from EMD Millipore (Billerica, MA, USA). The anti-LRP Heavy Chain mouse antibody (8G1) was from Calbiochem (San Diego, CA, USA). The PE mouse anti-human CD91 (LRP-1) antibody and PE mouse IgG1 κ isotype control were purchased from BD Biosciences (Mississauga, ON, Canada). Horseradish peroxidase-conjugated donkey anti-rabbit and anti-mouse IgG secondary antibodies were from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). Ultracel 10 K centrifugal filters were from Merck Millipore (Etobicoke, ON, Canada). All other reagents were from Sigma-Aldrich Canada.

Electrophoresis reagents were obtained from Bio-Rad Laboratories (Hercules, CA, USA). The protein content of cell lysates was assessed with the bicinchoninic acid kit from Sigma Chemical Co (St. Louis, MO, USA). ZA was a kind gift from Novartis (Basel, Switzerland). SIM and Y276 were purchased from Calbiochem (San Diego, CA, USA). When not otherwise specified, all the other reagents were purchased from Sigma Chemical Co.

Dulbecco’s Modified Eagle’s Medium (DMEM, with high or low glucose), fetal bovine serum (FBS), L-glutamine, penicillin and streptomycin, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl formazan (MTT), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid hydrate (Ferrozine^®), Folin-Ciocalteau reagent, gallic acid, ascorbic acid, Oil red O solution, Coomassie Brilliant Blue G-250, gelatin, and all chemicals and solvents were purchased from Merck KGaA (Darmstadt, DA, Germany). Electrophoresis reagents were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Primary antibodies were supplied by Cell Signaling Technology (Beverly, MA, USA), Molecular Probes^TM (Invitrogen, Carlsbad, CA, USA), and Santa Cruz (Heidelberg, Germany) (Table 3). HRP-linked anti-mouse IgG and anti-rabbit IgG secondary antibodies were obtained from Molecular Probes^TM (Invitrogen, Carlsbad, CA, USA). Disposable plastic was provided by Sarstedt (Milan, Italy).