L-glutamine, penicillin /streptomycin, DMEM without glucose, DMEM without
sodium pyruvate for H2O2 treatment medium, PVDF membrane, DMSO cell culture
grade, Lipofectamine 2000, Lipofectamine 3000 were purchased from Thermo
Fisher Scientific. Polyfect was purchased from qiagen. Antibodies for actin,
LC3, ATG5, myc, VPS34, ULK ser 317, ULK ser 757, ATG 13, ATG 13pSer 318,
Beclin1, Beclin Ser 15, VPS34 Ser 249, AMPK, PAMPK Thr 172,Flag, GST,
HA,FIP200, ATG101 were purchased from Cell Signaling Technology. ULK1ser555
and ULK ser 777 were from Millipore. ULK1 for immunoprecipitation was
purchased from Sigma. ULK1 for western blots was purchased from Santacruz
Biotechnology. Anti-mouse IPMK was developed in our lab.