Ab89831

Manufactured by Abcam

Ab89831 is a lab equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin, by Merck Group (1 mentions) Sulfolink beads, by Thermo Fisher Scientific (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti phospho histone h3 ser10, by Merck Group (1 mentions) Ab126760, by Abcam (1 mentions) Anti symplekin, by BD (1 mentions) Ab18058, by Abcam (1 mentions) Anti α tubulin clone dm1a, by Merck Group (1 mentions) Anti ha clone 3f10, by Merck Group (1 mentions)

2 protocols using ab89831

Antibody Generation and Characterization for Western Blot and Immunofluorescence

Cited 1 time

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The anti-PARN antibody for western blot analysis was raised against purified, recombinant His-PARN and affinity purified with the antigen coupled to SulfoLink beads (Thermo Fisher Scientific). The anti-C21orf70 antibody was raised against a C-terminal peptide (C-GQTLARQMQLEDGGQL) and purified as described above. The antibody against RPL23a has been raised as previously described (40 (link)). The antibodies against DIM2, ENP1, NOB1, RPS3 (34 (link)), NOC4L, RRP12, RPS19 (29 (link)) and TSR1 (41 (link)) have been described previously. Anti-PARN for immunofluorescence was purchased from Abcam (ab89831), anti-β-actin and anti-α-tubulin from Sigma Aldrich (A1987 and T5168), anti-CK1δ and anti-HNRNPC from Abcam (AF12G4 and ab10294), anti-CK1ε (610445), anti-HA from Covance (MMS-101P) and anti-RPL10 from Santa Cruz Biotechnologies, Inc. (sc-798). Secondary antibodies for immunofluorescence were purchased from Invitrogen.

Montellese C., Montel-Lehry N., Henras A.K., Kutay U., Gleizes P.E, & O’Donohue M.F. (2017). Poly(A)-specific ribonuclease is a nuclear ribosome biogenesis factor involved in human 18S rRNA maturation. Nucleic Acids Research, 45(11), 6822-6836.

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Detailed Antibody Protocol for Western Blotting

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Anti-HA (clone 3F10, Sigma-Aldrich), anti-α-Tubulin (clone DM1A, Sigma-Aldrich), anti-Cdk1 (ab18, Abcam, Cambridge, UK), anti-ERK1/ERK2 (9102, Cell Signaling, Danvers, MA), anti-xGLD2 (rabbit polyclonal antibody raised against KRRSDEGNSPYDVKC peptide, X. laevis), anti-4E-T (2297, Cell Signaling), anti-DDX6 (PD009, MBL, Nagoya, Japan), anti-hCPEB4 for WB (rabbit polyclonal antibody raised against amino acids 1–302), anti-hCPEB4 for IP (ab83009, Abcam), anti-phospho Histone H3 (Ser 10) (06–570, Merck Millipore, Billerica, MA), anti-Symplekin (610644, BD Transduction Laboratories, Franklin Lakes, NJ), anti-CPSF2 (ab126760, Abcam), anti-PARN (ab89831, Abcam), anti-hGLD2 (AP5092, Abgent, San Diego, CA), anti-S6 (2317, Cell Signaling), anti-Vinculin (ab18058, Abcam).

Guillén-Boixet J., Buzon V., Salvatella X, & Méndez R. (2016). CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. eLife, 5, e19298.

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