Brahms copeptin proavp kryptor

Venous blood sampling after 12-h fasting was performed in all the study participants for routine analyses and metabolic characterization. Total cholesterol (mg/dL), high-density lipoprotein cholesterol (HDL, mg/dL), triglycerides (mg/dL), aspartate aminotransferase (AST, IU/L), alanine aminotransferase (ALT, IU/L), gamma-glutamyl transpeptidase (GGT, mg/dL), fasting blood glucose (FBG, mg/dL), uric acid (mg/dL), and creatinine (mg/dL) were measured by centralized standard methods. Fasting insulin (FBI, μΙU/mL) was measured by radioimmunoassay (ADVIA Insulin Ready Pack 100; Bayer Diagnostics, Milan, Italy), with intra- and inter-assay coefficients of variation < 5%, by an experienced lab technician, at Sapienza University, Rome, Italy. Low-density lipoprotein (LDL) cholesterol value was obtained using the Friedewald formula; the homeostasis model assessment of insulin resistance (HOMA-IR) and insulin secretion (HOMA-β%) was calculated as previously described [29 (link)].
Fasting copeptin was measured in plasma samples frozen immediately after separation and stored at − 80 °C for some weeks, using a KRYPTOR Compact Plus device and commercially available chemiluminescence sandwich immunoassay copeptin ProAVP kit with coated tubes (Thermo Scientific BRAHMS Copeptin proAVP KRYPTOR).

Fasting plasma (fp) copeptin concentration was measured by using a KRYPTOR Compact Plus device and commercially available chemiluminescence sandwich immunoassay copeptin ProAVP kit with coated tubes from samples stored at − 80 °C (BRAHMS Copeptin proAVP KRYPTOR; ThermoFisher Scientific). Plasma glucose, ACTH and cortisol, serum insulin and urine cortisol measurements were performed at the certified University Hospital’s central clinical laboratory. Plasma glucagon concentration was measured at the Biomedical centre, Lund University, by using a commercially available immunoassay from samples stored at − 80 °C (Mercodia sandwich ELISA).