Anti-α-SMA (cs19245), anti-p-AMPKα (cs2535), anti-caspase-1 (cs24232) and anti-NOD-like receptor thermal protein domain associated protein 3 (NLRP3) (cs15101) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-CollagenI (ab34710), anti-Tissue Inhibitors of Metalloproteinase 1 (TIMP1) (ab61224), anti-caspase-3 (ab179517), anti-beclin-1 (ab210498), anti-Bcl-2 (ab194583) antibodies and the horseradish peroxidase (HRP)-conjugated goat anti-rabbit (ab97051) were purchased from Abcam (Cambridge, MA, USA). Anti-CollagenI (14695-1-AP), anti-SIRT3 (10099-1-AP), anti-F4/80 (28463-1-AP), anti-caspase-6 (10198-1-AP), anti-caspase-9 (10380-1-AP) and anti-GAPDH (60004-1-Ig) antibodies were purchased from Proteintech (Wuhan, HubChina). Anti-Matrix metalloproteinase-13 (MMP-13) (sc515284) and anti-Interleukin (IL)-1R1 (sc393998) antibodies were purchased from Santa Cruz Biotechnology (Stanta Cruze, CA, USA). Anti-IL-1β (mab4012) antibodies were purchased from R&D Systems (Minneapolis, MN, USA). Alexa Fluor 488 Goat anti-Mouse IgG (A11034) and Alexa Fluor Plus 647 Goat anti-Rabbit IgG (A32727) were purchased from Beyotime Biotechnology (Shanghai, China).
Bcl 2 ab194583
Manufactured by Abcam
Sourced in United States
Bcl-2 (ab194583) is a recombinant protein that corresponds to a portion of the Bcl-2 protein. Bcl-2 is a member of the Bcl-2 protein family and plays a role in regulating apoptosis, or programmed cell death.
Automatically generated - may contain errors
Lab products found in correlation
Ab61224, by Abcam (1 mentions)
Ab179517, by Abcam (1 mentions)
Ab210498, by Abcam (1 mentions)
Anti gapdh 60004 1 ig, by Proteintech (1 mentions)
Anti collageni ab34710, by Abcam (1 mentions)
Envision hrp kit, by Agilent Technologies (1 mentions)
Application software, by Leica (1 mentions)
Nb100 56113, by Novus Biologicals (1 mentions)
Ab6720, by Abcam (1 mentions)
Ab32503, by Abcam (1 mentions)
Cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions)
3 protocols using bcl 2 ab194583
1
Antibody Resource for Cellular Analysis
2
Immunohistochemical Analysis of Renal Proteins
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Preparation for immunohistochemical staining, including sections deparaffinization, and antigens retrieving was conducted. After blocking non-specific protein binding, sections were washed by PBS and incubated overnight (at 4 °C) with the primary rabbit antibody against rat; cleaved caspase-3 (NB100-56113) from Novus Biologicals, (dilution 1:1000), or Bcl-2 (ab194583) from Abcam Co (Cambridge, MA, USA), (dilution 1:100). Then, sections were washed by PBS, incubated with secondary antibody; HRP Envision kit (DAKO), for 20 min, washed by PBS, and positive immunoreactivities were developed by DAB visualization for 10 min and counter-staining with hematoxylin. Quantitative analysis was performed according to El-Nabarawy et. al. (2020) for determination of area percentage of immunohistochemical expression levels of indicated proteins, as estimated from six representative randomly selected fields in the tissue section using Leica application software (Leica Microsystems GmbH, Wetzlar, Germany) [33 (link)]. Statistical analysis of renal immuno-expressions in different groups was carried out using chi-squared "χ2" test. Representative microscopic images (× 400) were shown in the study.
3
Immunohistochemical Analysis of Brain Tissue
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For immunohistochemical analysis, the paraffin sections of brain tissues were deparaffinized, blocked with 5% BSA, and treated with rabbit anti-rat Bax (ab32503), Bcl-2 (ab194583) (Abcam, Cambridge, Massachusetts, USA), cleaved caspase-3 (#9661) (Cell Signaling Technology Inc., Beverly, Massachusetts, USA) and TRPV1 (PA5-77317) (Invitrogen, Carlsbad, CA, USA) at 37 °C for 2 h, separately. The sections were incubated with goat anti-rabbit second antibody (ab6720, Abcam) at 37 °C for 30 min. Then, SABC complex was added and the slides were stained with DAB solution. Finally, the slides were observed with a microscope (Olympus BX40, Tokyo, Japan). Cells with brown granule staining of the membrane/cytoplasm were considered positive. Under ×400 magnification, the morphometric examination was performed and the positive cell count was determined in a blinded manner by two independent investigators. For each section, five visual fields were chosen at random and mean number of the positive cells were represented.
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