Omnic series software

Manufactured by Thermo Fisher Scientific

Sourced in United States

OMNIC Series Software is a software package designed for controlling Thermo Scientific Fourier Transform Infrared (FT-IR) spectrometers. The software enables users to acquire, process, and analyze infrared spectroscopic data.

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OMNICTM Series OMNIC software OMNIC

22 protocols using omnic series software

FTIR Analysis of FNB-Carrier Interactions

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The intermolecular interactions between FNB and the carrier matrix were investigated using an FTIR analysis (iS50 FTIR equipped with a SMART OMNI-Sampler, ThermoFisher Scientific, Waltham, MA, USA). The FNB, HPMC AS LG, PM, and crushed EXT were analyzed from 4000–700 cm⁻¹, at a resolution of 4 cm⁻¹ for % transmittance with 64 scans per run. The background was collected before every run. Weak intermolecular interactions were analyzed and assessed on the OMNIC^TM series software (ThermoFisher Scientific, Waltham, MA, USA) for the collected spectra.

Kulkarni V.R., Chakka J., Alkadi F, & Maniruzzaman M. (2023). Veering to a Continuous Platform of Fused Deposition Modeling Based 3D Printing for Pharmaceutical Dosage Forms: Understanding the Effect of Layer Orientation on Formulation Performance. Pharmaceutics, 15(5), 1324.

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Infrared Spectroscopy of Material Compositions

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The infrared spectra of each composition were recorded for reference using a Nicolet 6700 FTIR-ATR Spectrometer (ThermoFisher Scientific Inc., Waltham, MA, USA) with a smart iTX Accessory. Sixteen scans were taken per sample from 4000 to 400 cm⁻¹ at a resolution of 4 cm⁻¹. The visualisation and collection of the collected data were performed using the OMNIC^TM Series software (ThermoFisher Scientific Inc., Waltham, MA, USA).

Voignac D., Belsey S., Wermter E., Paltiel Y, & Shoseyov O. (2023). Biobased Electronics: Tunable Dielectric and Piezoelectric Cellulose Nanocrystal—Protein Films. Nanomaterials, 13(15), 2258.

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Investigating Polymer-Drug Interactions via FT-IR

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The intermolecular interactions between the polymers (HPMC-AS HG, MG, and LG) and the drug were investigated using FT–IR analysis (iS50 FT–IR equipped with a SMART OMNI-Sampler, Nicolet, ThermoFisher Scientific, Waltham, MA, USA). Then, 20–25 mg of samples of pure drug, pure polymers, drug-polymer physical mixtures, and powdered extruded filaments were analyzed for % transmittance from 3100–700 cm⁻¹, at a resolution of 4 cm⁻¹ (64 scans per run), and background was collected before every run. The collected spectra were analyzed and assessed for weak intermolecular interaction on the OMNIC^TM series software (ThermoFisher Scientific, Waltham, MA, USA).

Thakkar R., Pillai A.R., Zhang J., Zhang Y., Kulkarni V, & Maniruzzaman M. (2020). Novel On-Demand 3-Dimensional (3-D) Printed Tablets Using Fill Density as an Effective Release-Controlling Tool. Polymers, 12(9), 1872.

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Protein Secondary Structure Analysis

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Protein secondary structure: All scaffolds (n = 3) were lyophilized prior to FTIR analysis. FTIR spectra were then recorded using a Thermo 6700 FTIR instrument, equipped with a Smart iTR Attenuated Total Reflectance (ATR) diamond plate, in the wave-number range 500–3500 cm⁻¹ (64 scans at a resolution of 4 cm⁻¹). Data were evaluated using OMNIC^TM series software (version 8, Thermo Scientific). The secondary structures of collagen proteins were determined using the spectral curve fitting of amide I region (1,700–1,600 cm⁻¹) and band narrowing of the spectrum by Fourier deconvolution followed by peak choosing and fitting. Six peaks were chosen and assigned to a specific secondary structure according to the literature for the original D-patch followed by peak fitting for the other two scaffolds.

Efraim Y., Schoen B., Zahran S., Davidov T., Vasilyev G., Baruch L., Zussman E, & Machluf M. (2019). 3D Structure and Processing Methods Direct the Biological Attributes of ECM-Based Cardiac Scaffolds. Scientific Reports, 9, 5578.

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Doxorubicin Attachment to MPI

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Attachment of doxorubicin to MPI was confirmed with Fourier transform infrared spectroscopy (FTIR) using a Thermo Electron Corporation Nicolet 6700 spectrophotometer (Medison, WI, USA) with a DGTS TEC detector using 64 scans at a resolution of 4 cm⁻¹. Data manipulation was conducted using OMNIC^TM series software (Thermo Fisher Scientific, Waltham, MA, USA).

Wang L., Song Y., Parikh A., Joyce P., Chung R., Liu L., Afinjuomo F., Hayball J.D., Petrovsky N., Barclay T.G, & Garg S. (2019). Doxorubicin-Loaded Delta Inulin Conjugates for Controlled and Targeted Drug Delivery: Development, Characterization, and In Vitro Evaluation. Pharmaceutics, 11(11), 581.

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FTIR Characterization of Leaf Extracts

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FTIR spectra of the extracts were performed to identify the present characteristic functional groups. The spectra were recorded using a Nicolet^TM iS^TM IR-spectrometer equipped with a smart iTX^TM accessory (Thermo Fischer Scientific, USA) in the wavenumber range between 4000–400 cm⁻¹, and a resolution of 4 cm⁻¹ at 25 ± 5 °C. The analysis was conducted on dry leaf extracts (Figure S1, Supplementary material), previously prepared using a Rotary vacuum evaporator (Rotavapor Heidolph 4001-efficient, Heidolph Instruments, Germany) under pressure of 0.25 bar at 25 ± 5 °C. Instrumental analysis was performed by fixing a small amount of the extract (20–30 mg) to the metal chassis of the IR-spectrometer equipped with diamond crystal. FTIR spectra were obtained by OMNIC^TM series software (Thermo Fischer Scientific, USA). The graphical view of results was generated by ORIGIN^TM (Version 9.0, OriginLab Corporation, USA).

Batinić P., Jovanović A., Stojković D., Čutović N., Cvijetić I., Gašić U., Carević T., Zengin G., Marinković A, & Marković T. (2024). A novel source of biologically active compounds – The leaves of Serbian herbaceous peonies. Saudi Pharmaceutical Journal : SPJ, 32(6), 102090.

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Investigating Copper-CPC Interactions via FTIR

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The intermolecular interactions between copper and CPC were investigated using FTIR spectroscopy (iS50 FT–IR) equipped with a SMART OMNI-Sampler (Nicolet, Thermo Fisher Scientific, Waltham, MA, USA). The scaffolds were powdered, and around 10–15 mg was used for FTIR analysis. The % transmittance was analyzed from 4000 to 400 cm⁻¹, at a resolution of 4 cm⁻¹ (64 scans per run) and the background was collected prior to every sample run. The spectra were analyzed using the OMNICTM series software, (Thermo Fisher Scientific, USA).

Pillai A., Chakka J., Heshmathi N., Zhang Y., Alkadi F, & Maniruzzaman M. (2023). Multifunctional Three-Dimensional Printed Copper Loaded Calcium Phosphate Scaffolds for Bone Regeneration. Pharmaceuticals, 16(3), 352.

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FT-IR Analysis of IND Formulations

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FT-IR provides insight into the post-processing interactions between different functional groups present on the components. FT-IR analysis was used to investigate the changes in the IND spectrum after each process and the interactions between IND and other components (iS50 FT-IR equipped with a SMART OMNI-Sampler, Nicolet, ThermoFisher Scientific, Waltham, Massachusetts, USA). A sample of 20-25 mg of IND, excipients, PM-I, granules, PM-II, LS printed tablets, and extruded filaments (powdered for the analysis) were dispensed on the sample holder and their % transmittance was measured using a range of 3100-700 cm -1 . The resolution of the test was set to 4 cm -1 with 64 scans per run. To ensure the absence of contamination from previous samples, the cell was cleaned using isopropanol and the background spectrum was collected between each sample. The raw data were translated into spectra which were then investigated for intermolecular interaction, stability, and the solid-state of the samples using OMNIC TM series software (ThermoFisher Scientific, Waltham, Massachusetts, USA).

Thakkar R., Zhang Y., Zhang J, & Maniruzzaman M. (2021). Synergistic application of twin-screw granulation and selective laser sintering 3D printing for the development of pharmaceutical dosage forms with enhanced dissolution rates and physical properties. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 163.

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Functional Group Identification of Powders

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A Fourier transform infrared (FTIR) spectroscopy was performed to identify the main functional groups of the powders, using a spectrometer (Thermo Fisher Scientific Nicolet S10 FT-IR, USA) in the range of 400 to 4000 1/cm. Sixty-four scans were performed per sample by duplicate at a resolution of 4 1/cm. Spectra analysis was performed using the OMNIC TM series software (Thermo Fisher Scientific, USA).

Irigoiti Y., Yamul D.K, & Navarro A.S. (2022). Co-crystallized sucrose with propolis extract as a food ingredient: Powder characterization and antioxidant stability. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 143.

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Functional Group Identification of Powders

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Irigoiti Y., Yamul D.K., & Navarro A.S. (2021). Co-crystallized sucrose with propolis extract as a food ingredient: Powder characterization and antioxidant stability. LWT, 143, 111164-111164.

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