β actin antibody

Manufactured by HuaAn Biotechnology

Sourced in China

The β-actin antibody is a laboratory reagent used to detect the presence and quantify the levels of the β-actin protein in biological samples. β-actin is a highly conserved and ubiquitously expressed cytoskeletal protein that is commonly used as a control or reference protein in various analytical techniques, such as Western blotting, immunohistochemistry, and flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Cyclohexane, by Merck Group (1 mentions) Ubiquitin antibody, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Traf6 antibody, by Abcam (1 mentions) P22077, by Selleck Chemicals (1 mentions) Phospho mapk family antibody sampler kit, by Cell Signaling Technology (1 mentions) Nf κb pathway sampler kit, by Cell Signaling Technology (1 mentions) Phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Phospho akt ser473 4058, by Cell Signaling Technology (1 mentions) Ne per nuclear and cytoplasmic extraction reagents kit, by Thermo Fisher Scientific (1 mentions) Lamin b1 antibody, by Proteintech (1 mentions)

Spelling variants of this product

β-actin (5 citations) Anti-β-actin antibody (3 citations)

3 protocols using β actin antibody

Cellular Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

P22077 was purchased from SelleckChem. Phospho-MAPK Family Antibody Sampler Kit, NF-κB Pathway Sampler Kit, pro-IL-1β antibody and ubiquitin antibody were purchased from Cell Signaling Technology. TRAF6 antibody was purchased from Abcam. Flag and HA tag antibodies were purchased from GenScript. β-actin antibody was purchased from HuaAn Biotechnology. DMSO, cyclohexane and LPS were obtained from Sigma-Aldrich.

Zhao X.B., Ji F.Y., Li H.R., Zhu H.H., Zhao Z.Z., Ling J., Di Q.Q., Ma X.Y, & Chen W.L. (2020). P22077 inhibits LPS-induced inflammatory response by promoting K48-linked ubiquitination and degradation of TRAF6. Aging (Albany NY), 12(11), 10969-10982.

+ Open protocol

+ Expand

Protein Expression and Signaling Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total protein of transfected cells was extracted and quantified. Proteins were then transferred and blocked for 2 h in 5% fat‐free milk at room temperature. Proteins were incubated with primary antibody overnight at 4°C, and incubated with secondary antibodies for 1 h. Images were captured and analyzed the density of band. ZW10 antibody (ab21582, Abcam) was obtained from Abcam, and the antibodies of PI3 kinase p85 (PI3K) (4292), AKT serine/threonine kinase 1 (Akt) (2938), Phospho‐Akt (Ser473) (4058), mechanistic target of rapamycin kinase (mTOR) (2983), and Phospho‐mTOR (Ser2448) (5536) were obtained from Cell Signaling Technology. The β‐actin antibody was obtained from HuaAn Biotechnology (JF53‐10).

Dang Y., Hu D., Xu J., Li C., Tang Y., Yang Z., Liu Y., Zhou W., Zhang L., Xu H., Xu Y, & Ji G. (2020). Comprehensive analysis of 5‐hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer. Clinical and Translational Medicine, 10(3), e125.

+ Open protocol

+ Expand

Nuclear and Cytoplasmic Fractionation of HCC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nuclear and cytoplasmic fractionations of tumor tissues of HCC were extracted using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer's protocol. Protein concentration was quantified using Pierce BCA Protein Assay. Ten microgram of each cytoplasmic and nuclear extract sample was analyzed for western blot with TERT antibody (diluted 1:500, Thermo Fisher Scientific, Rockford, USA), laminB1 antibody (diluted 1:1000, Proteintech, Hubei, China) and β-actin antibody (diluted 1:1000, HuaAn Biotechnology, Zhe Jiang, China). β-actin was used as cytoplasmic loading control for cytoplasmic extracts and laminB was used as nuclear loading control for nuclear extracts.

Huang W., Zhou W., Li C., Yang Y., Shang Y.K., Chen C., Zhang J., Yao R., Wang P., Wen W., Liu H.Q., Wang L., Li X., Bian H, & Chen Z.N. (2017). Promoter mutations and cellular distribution of telomerase in non-clear cell and clear cell hepatocellular carcinoma. Oncotarget, 8(16), 26288-26297.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!