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Anti cd57 apc

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Anti-CD57-APC is a fluorescent-labeled antibody that binds to the CD57 antigen on the surface of human cells. It is a tool used for the identification and analysis of CD57-positive cells in research applications.

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Lab products found in correlation

Anti cd3 pe cy7, by BD (2 mentions) Anti nkg2d apc, by Miltenyi Biotec (1 mentions) Anti cd3 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd8 pe, by BD (1 mentions) Anti nk1.1 fitc, by BD (1 mentions) Facscalibur, by BD (1 mentions) Anti cd27 pe, by BD (1 mentions) Anti cd24 pe, by BD (1 mentions) Anti cd90 pe cy7, by BD (1 mentions) Anti cd130 bv421, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Fcs express software, by De Novo Software (1 mentions) Anti cd163 pe, by BD (1 mentions) Anti cd28 pe, by BD (1 mentions) Anti hla dr apc, by BD (1 mentions) Anti cd16 pe cy7, by BD (1 mentions) Anti cd4 alexa488, by BD (1 mentions) Anti cd62l apc, by BD (1 mentions) Anti cd8 apc cy7, by BD (1 mentions) Anti cd4 horizon v500, by BD (1 mentions)

Spelling variants of this product

CD57 APC (5 citations) Anti-CD57 (2 citations)

5 protocols using anti cd57 apc

1

CD8+NKG2D+ Cells Analysis in Tumor

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For CD8+NKG2D+ cells analysis, cells were harvested and stained with anti-CD3-FITC (E10472-1633, eBioscience, USA), anti-CD8-PE (561946, BD, USA), anti-NKG2D-APC (130-099-216, Miltenyi Biotec, Germany), anti-CD27-PE (560985, BD, USA), and anti-CD57-APC (560845, BD, USA) antibodies. To detect the infiltration of CD8 or NK cells in the tumor mass, tumors were removed and crushed into single cell suspension. Then cells were stained with mouse anti-CD8-PErCp (46-0083-80, eBioscience, USA) or anti-NK1.1-FITC (553164, BD, USA) antibodies. To confirm the depletion of CD8 or NK cells, cells were isolated from peripheral blood and stained with anti-CD8-PErCp (46-0083-80) or anti-NK1.1-PerCp antibodies (45-5941-82) (all from eBioscience, USA). The staining was performed on ice for 30 min. Samples were acquired on the FACS Calibur (BD, USA) and data was analyzed using FlowJo software (Version 8.5.3, Tree Star Inc., USA).
Xu C., Lu X., Liu W., Chen A., Meng G., Zhang H., Li B., Zhang Y., Wu J, & Wei J. (2018). CD8+ T cells mediate the antitumor activity of frankincense and myrrh in hepatocellular carcinoma. Journal of Translational Medicine, 16, 132.
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2

Evaluating Pluripotent Stem Cell Viability

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The viability of disaggregated primed and naive hESCs and hiPSCs was analyzed with a Life/Death Fixable kit (Invitrogen, Waltham, MA, USA) in a FACS Canto II (BD Biosciences, Franklin Lakes, NJ, USA). Cells were stained with anti-CD24 PE (BD Biosciences, clone ML5) and anti-CD57 APC (BD Bioscience, clone NK-1) primed markers with anti-CD75 FITC (BD Biosciences, clone LN1) and anti-CD130 BV421 (BD Biosciences, clone AM64) naive markers and anti-CD90 PE-Cy7 (BD Biosciences, clone 5E10) stemness marker. Approximately 30,000–50,000 events were acquired for analysis. Populations were analyzed using FlowJo v.X.0.7 (TreeStar Inc., Ashland, OR, USA). See Supplementary Table S1.
Romayor I., Herrera L., Burón M., Martin-Inaraja M., Prieto L., Etxaniz J., Inglés-Ferrándiz M., Pineda J.R, & Eguizabal C. (2022). A Comparative Study of Cell Culture Conditions during Conversion from Primed to Naive Human Pluripotent Stem Cells. Biomedicines, 10(6), 1358.
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3

Multicolor Flow Cytometry Panels

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The following flow cytometry antibodies were purchased from BD Biosciences (San Jose, CA): anti-CD3-PE Cy7, anti-CD4-Alexa488, anti-CD8-PacBlu, anti-CD28-PE, anti-CD57-APC, anti-CD45RO-Alexa 700, anti-HLA-DR-APC, anti-CD14-PacBlu, anti-CD11a-FITC, anti-CD16-PE-Cy7, anti-CD163-PE, anti-CD62L-APC, and anti-CD86-Alexa 700. Two multi-color panels were used for T cells, and two panels were used for monocytes. Data were collected using a BD LSRII flow cytometer and analyzed with FCS Express software (DeNovo Software, Los Angeles, CA).
Wallet M.A., Buford T.W., Joseph A.M., Sankuratri M., Leeuwenburgh C., Pahor M., Manini T., Sleasman J.W, & Goodenow M.M. (2015). Increased inflammation but similar physical composition and function in older-aged, HIV-1 infected subjects. BMC Immunology, 16, 43.
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4

Phenotypic Characterization of T-Cell Subsets

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Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using Ficoll-Hypaque (GE Healthcare, Sweden) and analyzed by flow cytometry. PBMCs were stained with fluorochrome-conjugated monoclonal antibodies against surface antigens for 30 min at 4 °C. The antibodies used included anti-CD3-PE-Cy7, anti-CD4-Horizon V500, anti-CD8-APC-Cy7, anti-CD31-FITC, anti-CXCR4-PerCP-Cy5.5, and anti-CD28-APC-H7 (all from BD Biosciences, San Jose, California, USA). Phenotypic characterization of CD28null-Tang and CD28+-Tang subsets was performed by extracellular staining with anti-CD57-APC, anti-CD27-APC, anti-CCR7-APC, or antihTERT-APC or the corresponding isotype controls (all from BD Biosciences). Multicolor flow cytometry was performed using an CytoFlex S (Beckman Coulter), and FlowJo V10 software (Treestar, San Carlos, California, USA) was used to analyze the data.
Zhang G., Liu Y., Qiu Y., Zhang J., Sun J., Zhou Z., Wang Z., Zeng P., Tao J, & He J. (2020). Circulating senescent angiogenic T cells are linked with endothelial dysfunction and systemic inflammation in hypertension. Journal of Hypertension, 39(5), 970-978.
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5

Isolation and Characterization of CMV-Specific CD8+ T Cells

Cited 1 time
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PE-conjugated HLA-A2 tetramers refolded around the CMV pp65495-503 epitope NLVPMVATV (NLV) were generated as described previously48 (link),49 (link) and used at a final concentration of 10 μg/mL. Directly conjugated mAbs were obtained from commercial suppliers: (i) anti-CD3-APC-H7, anti-CD8-BV786, anti-CD14-BV510, anti-CD19-BV510, anti-CD45-RA-PerCP-Cy5.5, anti-CD57-APC, anti-CD127-BV421, anti-CD160-PerCP-Cy5.5, anti-CCR7-PE-Cy7, anti-CTLA-4-PE-Cy7, anti-BTLA-APC, anti-2B4-FITC, anti-2B4-PE, and anti-Tim-3-AF700 (BD Biosciences); (ii) anti-CD27-BV605 and anti-PD-1-PE/Dazzle 594 (BioLegend); and (iii) a panel of anti-TCR-Vβ-FITC and anti-TCR-Vβ-PE mAbs (Beckman Coulter). The acute effects of dasatinib administration were monitored using an IOTest Beta Mark TCR Repertoire Kit (BD Biosciences). Non-viable cells were excluded from the analysis using LIVE/DEAD Fixable Aqua (Life Technologies). Data were acquired using a Fortessa X-20 flow cytometer (BD Biosciences) and analyzed with FlowJo software version 10.0.7 (Tree Star). Viable CD3+ CD8+ TCR-Vβ+ cells were sorted using an InfluxTM Cell Sorter (BD Biosciences).
Lissina A., McLaren J.E., Ilander M., Andersson E.I., Lewis C.S., Clement M., Herman A., Ladell K., Llewellyn-Lacey S., Miners K.L., Gostick E., Melenhorst J.J., Barrett A.J., Price D.A., Mustjoki S, & Wooldridge L. (2018). Divergent roles for antigenic drive in the aetiology of primary versus dasatinib-associated CD8+ TCR-Vβ+ expansions. Scientific Reports, 8, 2534.
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