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E el m0730c

Manufactured by Elabscience
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Sourced in China, United States

The E-EL-M0730c is a lab equipment product from Elabscience. It is designed for general laboratory use. The core function of this product is not available.

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Lab products found in correlation

E el m0037c, by Elabscience (3 mentions) A003 1 2, by Nanjing Jiancheng (1 mentions) A001 3 2, by Nanjing Jiancheng (1 mentions) A005 1 2, by Nanjing Jiancheng (1 mentions) E el m0044c, by Elabscience (1 mentions) E el m3063, by Elabscience (1 mentions) E el m0048c, by Elabscience (1 mentions)

4 protocols using e el m0730c

1

Quantifying Serum IL-1β and IL-18 in MI Mice

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After various treatments, the concentration of IL-1β (E-EL-M0037c, Elabscience, Wuhan, China) and IL-18 (E-EL-M0730c, Elabscience, Ltd, Wuhan, China) in the serum of MI mice was measured by ELISA kit according to the protocols25 (link). Absorbance at 450 nm was measured in each well by visualization of color intensity development.
Li Z., Xu H., Liu X., Hong Y., Lou H., Liu H., Bai X., Wang L., Li X., Monayo S.M., Mokembo J.N., Jha N.K., Yang B, & Zhang Y. (2020). GDF11 inhibits cardiomyocyte pyroptosis and exerts cardioprotection in acute myocardial infarction mice by upregulation of transcription factor HOXA3. Cell Death & Disease, 11(10), 917.
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2

Oxidative Stress and Neuroinflammation Markers

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Plasma was collected, and the levels of total superoxide dismutase (SOD, A001-3-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), malondialdehyde (MDA, A003-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and glutathione peroxidase (GSH-Px, A005-1-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) were measured using the corresponding kit according to the manufacturer’s instructions. The levels of Aβ1–40 (E-EL-M300, Elabscience Biotechnology Co., Ltd., Wuhan, China), Aβ1–42 (E-EL-M3010, Elabscience Biotechnology Co., Ltd., Wuhan, China), p-Tau (E-EL-M1289c, Elabscience Biotechnology Co., Ltd., Wuhan, China), IL-1β (E-EL-M0037c, Elabscience Biotechnology Co., Ltd., Wuhan, China), and IL-18 (E-EL-M0730c, Elabscience Biotechnology Co., Ltd., Wuhan, China) in the supernatant from the brain homogenate were assessed using the corresponding enzyme-linked immunosorbent assay (ELISA) kits following the manufacturer’s protocols.
Guo P., Zeng M., Wang S., Cao B., Liu M., Zhang Y., Jia J., Zhang Q., Zhang B., Wang R., Zheng X, & Feng W. (2022). Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25–35-Induced Mice by Inhibiting the NLRP3 Inflammasome. Molecules, 27(8), 2488.
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3

ELISA for Inflammatory Proteins

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Protein of interest in serum or cell supernatant was detected by ELISA according to the manufacturer’s instructions. Detailed ELISA kits were as follows: Mouse S100A8 (E-EL-M3048, elabscience, Wuhan, Hubei, China), mouse IL-1β (VAL601, Novus, Littleton, CO, USA), mouse IL-18 (E-EL-M0730c, elabscience, Wuhan, Hubei, China), mouse GSDMD (JL46371-96T, JiangLai, Baoshan, Shanghai, China), human S100A8 (E-EL-H1289c, elabscience, Wuhan, Hubei, China), human IL-1β (Mengbio, Shapingba, Chongqing, China), human IL-18 (Mengbio, Shapingba, Chongqing, China), and human GSDMD (Mengbio, Shapingba, Chongqing, China).
Liu Y., Kong X., You Y., Xiang L., Zhang Y., Wu R., Zhou L, & Duan L. (2022). S100A8-Mediated NLRP3 Inflammasome-Dependent Pyroptosis in Macrophages Facilitates Liver Fibrosis Progression. Cells, 11(22), 3579.
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4

Cytokine Profiling in Colon Tissue

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The colon tissues were homogenized in PBS on ice and then centrifugated at 500 g for 10 min at 4 °C and the supernatant was collected and analyzed. The cell culture medium was centrifugated at 1000 g for 20 min at 4 °C to remove impurities and cell debris, and the supernatant was collected and analyzed. The levels of TNF-α (E-EL-M3063; Elabscience, Houston, TX, USA), IFN-γ (E-EL-M0048c; Elabscience), IL-6 (E-EL-M0044c; Elabscience), IL-1β (E-EL-M0037c; Elabscience), IL-18 (E-EL-M0730c; Elabscience), and IL-17 (BMS6001TEN; Invitrogen) were determined using commercial ELISA kits. The optical density was measured at 450 nm using a microplate reader.
Chen M., Chen Y., Fu R., Liu S., Li H, & Shen T. (2024). Atox1 regulates macrophage polarization in intestinal inflammation via ROS-NLRP3 inflammasome pathway. Journal of Translational Medicine, 22, 497.
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