Trpv1 cre

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TRPV1-Cre is a transgenic mouse line that expresses Cre recombinase under the control of the transient receptor potential cation channel, subfamily V, member 1 (Trpv1) promoter. Trpv1 encodes the capsaicin receptor, which is expressed in a subset of sensory neurons. This mouse line can be used to genetically manipulate gene expression in a Trpv1-positive cell population.

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14 protocols using trpv1 cre

Genetic Manipulation of Mouse Neuronal Subtypes

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All animal procedures were approved by the Institutional Animal Care and Use Committee of Brown University, Providence. Transient receptor potential vanilloid receptor 1 (Trpv1)‐Cre, vesicular GABA transporter (VGAT)‐Cre, somatostatin (SOM)‐cre, cyclin E2 (CCNE2)‐GFP, lox‐STOP‐lox‐ChR2‐EYFP, and lox‐STOP‐lox‐TdTomato mice were purchased from The Jackson Laboratory. Trpv1‐Cre^+/+ mice were mated with ChR2‐EYFP^+/+ mice to generate Trpv1^+/−/ChR2‐EYFP^+/− offspring (referred to as TRPV1/ChR2). TRPV1/ChR2 mice used in this study were first‐generation progeny of homozygous parents. Vgat‐cre^+/+ and Som‐cre^+/+ were mated with TdTomato^+/+ mice to generate Vgat‐cre^+/+/TdTomato^+/+ and Som‐cre^+/+/TdTomato^+/+ (referred to as VGAT‐ and SOM/TdTom, respectively). CCNE2‐GFP^+/+ mice were kept on a homozygous breeding schedule to generate CCNE2‐GFP^+/+ offspring. Both male and female mice were used for this study.

Pradier B., McCormick S.J., Tsuda A.C., Chen R.W., Atkinson A.L., Westrick M.R., Buckholtz C.L, & Kauer J.A. (2019). Properties of neurons in the superficial laminae of trigeminal nucleus caudalis. Physiological Reports, 7(12), e14112.

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Genetically Modified Mice for Neuroscience

Cited 3 times

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Mrgpra3^Cre-EGFP mice (Han et al., 2013 (link)) were imported from Dr. Qin Liu’s lab at the Washington University School of Medicine to Dr. Luo’s lab at the University of Pennsylvania. Rosa^ChR2f/f (JAX 012569), Vglut2^f/f (JAX 012898) (Tong et al., 2007 (link)), Trpv1^Cre (JAX 017769) (Cavanaugh et al., 2011 (link)), and Rosa^Tdtf/f (JAX 007909) (Madisen et al., 2010 (link)) mice were purchased from the Jackson Laboratories. The Nmbr^Cre knock-in mouse line was generated by Dr. Janardhan Bhattarai in the lab, using mouse fertilized oocyte injection of CRISPR/CAS9 mixture conducted by the Johns Hopkins Transgenic Core (details of this mouse line generation will be published in another manuscript). Animals were housed in facilities at the University of Pennsylvania and at the Washington University School of Medicine. All experiments were conducted in accordance with the National Institute of Health guidelines and with approval from the Institutional Animal Care and Use Committee of University of Pennsylvania and Washington University School of Medicine. Adult male and female mice with desired genotypes were kept in a standard 12-h light/dark cycle, with water and food pellets available ad libitum. Litters and animals were randomized at the time of assigning experimental conditions for the whole study.

Cui L., Guo J., Cranfill S.L., Gautam M., Bhattarai J., Olson W., Beattie K., Challis R.C., Wu Q., Song X., Raabe T., Gradinaru V., Ma M., Liu Q, & Luo W. (2022). Glutamate in Primary Afferents is Required for Itch Transmission. Neuron, 110(5), 809-823.e5.

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Behavioral Experiments on Genetically Modified Mice

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Mice were housed and bred at Brown University. All protocols and procedures were approved by the Brown University Institutional Animal Care and Use Committee. Mice were maintained at 22°C in a 12 hr light/dark cycle, with food and water available ad libitum. Trpv1^Cre (Cavanaugh et al., 2011 (link)) (Cat# JAX:017769, RRID:IMSR_JAX:017769), lox-STOP-lox^ChR2-EYFP (Cat# JAX:012569, RRID:IMSR_JAX:012569) and lox-STOP-lox^TdTomato (Madisen et al., 2012 (link)) (Cat# JAX:007908, RRID:IMSR_JAX:007908) mice were purchased from The Jackson Laboratory. Mice used in this study were first generation double heterozygous offspring from single homozygous parents. All procedures used to generate data reported in this study are described here. Mice had no prior history of drug administration, surgery or behavioral testing. Behavioral experiments were performed on male and female mice 3–5 month of age.

López Soto E.J, & Lipscombe D. (2020). Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and function. eLife, 9, e54879.

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Transgenic Neuronal Calcium Imaging

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Mice were housed in a temperature- and light-controlled room (23 ± 2°C and 12 h light/dark cycle, respectively) with ad libitum food (2918 Teklad global 18% protein rodent diet, Envigo) and water. TRPV1-Cre (stock number 017769) and GCaMP6f (stock number 028865) strains were purchased from Jackson Laboratories. Mice with p35 knockout or transgenic overexpression, i.e., p35^−/− background and Tgp35 mice, were generated in our laboratory (Pareek and Kulkarni, 2006 (link)). P35^−/− mice were maintained in a C57BL6/129SVJ background. Tgp35 mice and wild-type littermate controls were maintained in an FVBN background. For imaging, mice were crossed to generate a GCaMP6 and TRPV1-Cre line, which was then maintained with p35^−/− or tgp35 backgrounds. 78 mice were used (39 male and 39 female): C57BL6/129SVJ mice (JAX 000664, n = 18), TRPV1-Cre (+/−) GCaMP6(+/−) mice (n = 9), TRPV1-Cre (+/−) P35(−/−) GCaMP6 (+/−) mice (n = 9), TRPV1-Cre(+/−) GCaMP6(+/−)Tgp35 in FVBN background mice (n = 21) and TRPV1-Cre(+/−) GCaMP6(+/−) in FVBN background mice (n = 21). At the time of live imaging procedures, the mice were approximately 10–12 weeks old. For all experiments, age-matched wild-type littermates served as controls.

Hu M., Doyle A.D., Yamada K.M, & Kulkarni A.B. (2022). Visualization of trigeminal ganglion sensory neuronal signaling regulated by Cdk5. Cell reports, 38(10), 110458.

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Genetic Tools for Somatosensory Neuroscience

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ErbB4-2A-CreERT2 mice (#012360), Ai9 (Rosa-CAG-LSL-tdTomato) reporter mice (#007905), cFos::shEGFP mice (#018306), MrgprD-CreER (#031286), SST-Cre (#013044), CCK-Cre (#012706), Rosa-LSL-EYFP (#006148), Advillin-CreER (#032027) and TRPV1-Cre mice (#017769) were obtained from the Jackson Laboratory. ErbB4f/f mice, αMHC::ErbB4 mice, NRG1f/f, T796G, and stop-ErbB4 mice have been described previously (Garcia-Rivello et al., 2005 (link); Li et al., 2002 (link); Tidcombe et al., 2003 (link); Wang et al., 2018b (link); Wang et al., 2021 ). Mice were housed in an environment of 22 ± 2°C and 12 hr light/dark cycle with food and water ad libitum. Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Case Western Reserve University. Male mice were used in the present study. For the recording of Aβ, Aδ and C inputs, 20-30 day-old mice were used. For semi-intact recordings, 4-7 week-old mice were used. For recording in spinal cord slices, viral injections were performed in 2-4 week-old mice and recordings were performed 2-3 weeks later. For behavioral studies, mice of 2-5 month-old were used. Experimenters were blinded to genotypes and treatments.

Wang H., Chen W., Dong Z., Xing G., Cui W., Yao L., Zou W.J., Robinson H.L., Bian Y., Liu Z., Zhao K., Luo B., Gao N., Zhang H., Ren X., Yu Z., Meixiong J., Xiong W.C, & Mei L. (2022). A novel spinal neuron connection for heat sensation. Neuron, 110(14), 2315-2333.e6.

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Transgenic Mouse Strains for Neuroscience

Cited 2 times

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Wild-type C57BL/6J (000664), Glp1r-ires-Cre (029283), Gpr65-ires-Cre (029282), Npy2r-ires-Cre (029285), P2ry1-ires-Cre (029284), Agtr1a-Cre (030553), Calb2-ires-Cre (010774), Nts-Cre (017525), Vglut2-ires-Cre (016963), Sst-ires-Cre (013044), Twist2-Cre (008712), Vip-ires-Cre (010908), Piezo2-GFP-ires-Cre (027719), Trpv1-Cre (017769), Pvalb-Cre (017320), Vglut1-ires2-Cre (023527), Chat-ires-Cre (031661), lox-ChR2 (024109), lox-tdTomato (007914), and Snap25-2A-GCaMP6s-D (025111) were from the Jackson Laboratory. Drd2-Cre (032108-UCD) mice were from the Mutant Mouse Resource and Research Center (MMRRC). lox-L10-GFP mice were described before^{11 (link),12 (link)}.

Zhao Q., Yu C.D., Wang R., Xu Q.J., Dai Pra R., Zhang L, & Chang R.B. (2022). A multidimensional coding architecture of the vagal interoceptive system. Nature, 603(7903), 878-884.

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Trpv1-Cre/DTR Mice for TRPV1 Neuron Depletion

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Adult male C57BL/6J mice (6 to 8 weeks old) were obtained from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). Trpv1-Cre^+/+ (strain number: #017769) and ROSA26iDTR^+/+ mice (strain number: #007900) were acquired from the Jackson Laboratory (Bar Harbo, Maine, USA). To deplete TRPV1 neurons, Trpv1-Cre^+/+ mice were crossbred with ROSA26iDTR^+/+ mice, resulting in TRPV1 neuron-depleted mice (Trpv1-Cre/DTR). The mice were bred and housed in the animal center of the Eye Institute of Shandong First Medical University. All animal experiments were conducted in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the Ethics Committee of the Eye Institute of Shandong First Medical University.

Ma L., Yang L., Wang X., Zhao L., Bai X., Qi X., Chen Q., Li Y, & Zhou Q. (2024). CGRP Released by Corneal Sensory Nerve Maintains Tear Secretion of the Lacrimal Gland. Investigative Ophthalmology & Visual Science, 65(4), 30.

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Genetically Engineered Mouse Models for Neurobiology

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C57BL/6 (B6) (Jax no.000664), Trpv1-Cre (Jax no.017769), tdTomato^fl/stop/fl (Jax no.007914), hM4Di^fl-stop-fl (Jax no.026219), hM3Dq^fl-stop-fl (Jax no.026220) and Tac1^−/− (Jax no.004103) mice were purchased from The Jackson Laboratories and bred at specific pathogen-free facilities at Weill Cornell Medicine. Taconic C57BL/6 were purchased from Taconic Biosciences. Germ-free C57BL/6J were maintained at the Weill Cornell Medicine Gnotobiotic Mouse Facility. Germ-free C57BL/6J and microbial colonized ex-GF mice were maintained in sterile isocages during all experimental procedures. All mice used were between 6 and 16 weeks old and were age- and sex-matched for each experiment, maintained on a 12 hour light–dark cycle, and provided food and water ad libitum. Male and female mice were used for experiments; no differences were observed within the parameters analyzed. Littermates of the same sex were randomly assigned to experimental groups. All protocols were approved by the Weill Cornell Medicine Institutional Animal Care and Use Committee (IACUC), and all experiments were performed according to the guidelines of the IACUC.

Zhang W., Lyu M., Bessman N.J., Xie Z., Arifuzzaman M., Yano H., Parkhurst C.N., Chu C., Zhou L., Putzel G.G., Li T.T., Jin W.B., Zhou J., Hu H., Tsou A.M., Guo C.J, & Artis D. (2022). Gut-innervating nociceptors regulate the intestinal microbiota to promote tissue protection. Cell, 185(22), 4170-4189.e20.

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Genetic Labeling of Neuronal Subpopulations

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All experimental procedures were approved by the Universitat Autònoma de Barcelona Animal Experimentation Ethical Committee and followed the European Communities Council Directive 2010/63/EU and the Spanish National law (RD 53/2013). Mice were generated by breeding homozygous Ai9(RCL-tdT) mice (JAX stock #007909) (Madisen et al., 2010 (link)) with different homozygous Cre-driver lines from The Jackson Laboratory (Bar Harbor, ME, USA): ChAT-IRES-Cre (choline acetyltransferase, JAX stock #006410) (Rossi et al., 2011 (link)), B6 PVcre (parvalbumin, JAX stock #017320), Npy2r-IRES-Cre (neuropeptide Y receptor Y2, JAX stock #029285) (Barrozo et al., 2016 (link)), and TRPV1-Cre (transient receptor potential vanilloid 1, JAX stock #017769) (Cavanaugh et al., 2011 (link)). We obtained four mice lines that expressed the red fluorescent protein TdTomato under the control of a specific neuronal promoter: ChAT-Cre/Ai9, PV-Cre/Ai9, Npy2r-Cre/Ai9, and TRPV1-Cre/Ai9, respectively. The same Cre-driver lines were bred to homozygous Ribotag mice (Sanz et al., 2009 (link)). We obtained mice that expressed HA-tagged ribosomes in specific cell populations: ChAT-Cre/Ribotag, PV-Cre/Ribotag, Npy2r-Cre/Ribotag, and TRPV1-Cre/Ribotag, respectively. Mice were housed in a controlled environment (12 hr light-dark cycle, 22 ± 2°C), in open cages with water and food ad libitum.

Bolívar S., Sanz E., Ovelleiro D., Zochodne D.W, & Udina E. (2024). Neuron-specific RNA-sequencing reveals different responses in peripheral neurons after nerve injury. eLife, 12, RP91316.

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Retinal TRPV1 and TRPV4 Expression in Mice

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Animal handling, anesthetic procedures, and experiments were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. The project was approved by the Institutional Animal Care and Use Committees at the University of Utah. We assessed retinal TRPV1 expression using a knock-in mouse in which Cre was inserted into Exon 15 of Trpv1 (TRPV1Cre; Jackson Laboratory 017769). This line was crossed to B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9; 007909) in which the LoxP-STOP-LoxP TdTomato construct is knocked in at the Gt(ROSA)26Sor locus (Madison et al., 2010 (link); Jo et al., 2017 (link)). Trpv4^-/- mice have an excised exon 12-encoding transmembrane pore domains 5 and 6 (Liedtke and Friedman, 2003 (link)). C57BL/6J (C57), bacterial artificial chromosome (BAC)-transgenic Tg(TRPV4- EGFP)MT43Gsat mice (referred to as TRPV4^eGFP), TRPV1^-/-, TRPV4^-/-, TRPV1Cre:Ai3, and TRPV1Cre:Ai9 mice were maintained in a pathogen-free facility with a 12-h light/dark cycle and unrestrained access to food and water. Data were gathered from male and female mice with no noted gender differences.

Lakk M., Young D., Baumann J.M., Jo A.O., Hu H, & Križaj D. (2018). Polymodal TRPV1 and TRPV4 Sensors Colocalize but Do Not Functionally Interact in a Subpopulation of Mouse Retinal Ganglion Cells. Frontiers in Cellular Neuroscience, 12, 353.

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